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Discrimination Of Nine Crassostrea Oyster Species Based Upon Restriction Fragment-Length Polymorphism Analysis Of Nuclear And Mitochondrial Dna Markers, Jan F. Cordes, Jie Xiao, Kimberly S. Reece
Discrimination Of Nine Crassostrea Oyster Species Based Upon Restriction Fragment-Length Polymorphism Analysis Of Nuclear And Mitochondrial Dna Markers, Jan F. Cordes, Jie Xiao, Kimberly S. Reece
VIMS Articles
A molecular genetic identification key for nine species of Crassostrea oysters was developed based on restriction fragment-length polymorphism (RFLP) analyses of nuclear and mitochondrial DNA markers. Seven of nine species were unambiguosly differentiated based on digestion of the ITS-1 nuclear marker with Hae III an Hinf I. Individual species exhibited one or two RFLP patterns for each restriction endonuclease, and only C. madrasensis and C. iredalei were indistinguishable electrophoretically. All nine species were unambiguosly differentiated based on digestion of the COI mitochondrial marker with Dde I and Hae III. Species exhibited one or two RFLP patterns for each restriction endonuclease, …