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Full-Text Articles in Life Sciences
Investigation Of Rna Interference (Rnai)-Mediated Gene Silencing In Rainbow Trout, Sarah Au
Investigation Of Rna Interference (Rnai)-Mediated Gene Silencing In Rainbow Trout, Sarah Au
Theses and Dissertations (Comprehensive)
RNA interference (RNAi) was first characterized in plants and invertebrates as an antiviral innate immune response. It is a natural antiviral defence mechanism to degrade viral RNA by virus-induced gene silencing. Studies showed synthetic long double-stranded RNA (dsRNA; >30bp) degraded Caenorhabditis elegans messenger RNA (mRNA) transcripts, resulting in sequence-dependent gene silencing. Currently, there is only limited research on RNAi in fish, particularly the use of long dsRNA to trigger sequence-specific gene silencing. This is because studies have shown long dsRNAs trigger interferon response that could mask gene-specific RNAi effects. This thesis explored long dsRNA-mediated RNAi pathways in rainbow trout cells …
Using Phytoglycogen-Based Nanoparticles To Enhance Inosine Monophsophate Delivery Using Rainbow Trout And Bovine Models, Kristof Jenik
Using Phytoglycogen-Based Nanoparticles To Enhance Inosine Monophsophate Delivery Using Rainbow Trout And Bovine Models, Kristof Jenik
Theses and Dissertations (Comprehensive)
Recently, there has been interest in the use of nucleotides in industrials feeds, with inosine monophosphate showing the most promising effects on growth and immunity. Rainbow trout and Alberta cattle are two of Canadas most profitable farmed animals, if their growth rates and immunity could be enhanced this would further improve their value. The impacts of IMP, a phytoglycogen-based nanoparticle (NP) and inosine monophosphate cationically bound to the phytoglycogen-based nanoparticle (IMP-NP) were characterized in rainbow trout in vivo and in vitro and in vitro in bovine cells. Rainbow trout that were fed an IMP-NP containing feed on average had higher …
Investigating The Antiviral Effects Of Vig-3 From Rainbow Trout, Shanee Herrington-Krause
Investigating The Antiviral Effects Of Vig-3 From Rainbow Trout, Shanee Herrington-Krause
Theses and Dissertations (Comprehensive)
Rainbow trout is the most farmed fish in Ontario, and thus is economically important to the province. Despite this, there is a lack of understanding regarding fish innate immunity, specifically with regards to interferon-stimulated genes (ISGs) and their antiviral effector functions. ISGs are the workhorses of the innate antiviral response, operating together to limit each step of virus replication. The Viral Hemorrhagic Septicemia Virus (VHSV) induced gene (Vig)-3 is a newly identified ISG within many fish species and is homologous to ISG-15 in mammals. It is a small ubiquitin-like protein inducible by type I interferon (IFN-I), and is suggested to …
Characterizing Dsrna Production In Virus-Infected Fish Cells, Amal Brek Aloufi
Characterizing Dsrna Production In Virus-Infected Fish Cells, Amal Brek Aloufi
Theses and Dissertations (Comprehensive)
Viral dsRNA is produced by almost all viruses sometime during their replicative cycle. These viral nucleic acids are potent inducers of both innate and adaptive immune responses, and are therefore considered important immuno-modulators. Previous studies have shown that viruses produce dsRNA when replicating in mammalian cells; however, to date no one has demonstrated viral dsRNA production in virus infected fish cells. Therefore, the goal of this study is to investigate dsRNA production by fish viruses in fish cells, verifying production and performing initial characterization of the dsRNA molecules being produced. Three different rainbow trout cell lines were used in this …