Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
Investigation Of Rna Interference (Rnai)-Mediated Gene Silencing In Rainbow Trout, Sarah Au
Investigation Of Rna Interference (Rnai)-Mediated Gene Silencing In Rainbow Trout, Sarah Au
Theses and Dissertations (Comprehensive)
RNA interference (RNAi) was first characterized in plants and invertebrates as an antiviral innate immune response. It is a natural antiviral defence mechanism to degrade viral RNA by virus-induced gene silencing. Studies showed synthetic long double-stranded RNA (dsRNA; >30bp) degraded Caenorhabditis elegans messenger RNA (mRNA) transcripts, resulting in sequence-dependent gene silencing. Currently, there is only limited research on RNAi in fish, particularly the use of long dsRNA to trigger sequence-specific gene silencing. This is because studies have shown long dsRNAs trigger interferon response that could mask gene-specific RNAi effects. This thesis explored long dsRNA-mediated RNAi pathways in rainbow trout cells …
Using Phytoglycogen-Based Nanoparticles To Enhance Inosine Monophsophate Delivery Using Rainbow Trout And Bovine Models, Kristof Jenik
Using Phytoglycogen-Based Nanoparticles To Enhance Inosine Monophsophate Delivery Using Rainbow Trout And Bovine Models, Kristof Jenik
Theses and Dissertations (Comprehensive)
Recently, there has been interest in the use of nucleotides in industrials feeds, with inosine monophosphate showing the most promising effects on growth and immunity. Rainbow trout and Alberta cattle are two of Canadas most profitable farmed animals, if their growth rates and immunity could be enhanced this would further improve their value. The impacts of IMP, a phytoglycogen-based nanoparticle (NP) and inosine monophosphate cationically bound to the phytoglycogen-based nanoparticle (IMP-NP) were characterized in rainbow trout in vivo and in vitro and in vitro in bovine cells. Rainbow trout that were fed an IMP-NP containing feed on average had higher …