Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Evidence For Dna Methylation-Sensitive Transactivator Mediated Regulation Of The Expression Of An Epstein-Barr Virus Neutralizing Antigen, Sridhar Chalasani
Evidence For Dna Methylation-Sensitive Transactivator Mediated Regulation Of The Expression Of An Epstein-Barr Virus Neutralizing Antigen, Sridhar Chalasani
Masters Theses
DNA methylation has a profound effect on the expression of a number of eukaryotic genes. This study demonstrates that an Epstein-Barr virus (EBV) positive Burkitt's lymphoma cell line, Raji, contains the gene encoding gp350/220 but is not expressed. Frog virus 3 (FV3), an iridovirus, has the ability to overcome the inhibitory effect of DNA methylation. Fusion of FV3-infected cells with Raji cells resulted in expression of gp350/220, suggesting that gp350/220 is regulated by DNA methylation. Further evidence for this hypothesis was obtained by transfecting B95.8 cells with HpaII/HhaI methylated plasmid pLATE-CAT, containing the gp350/220 promoter. Southern blot analyses and a …
A Study Of Cell-Mediated Immunity During A Primary Infection With Pseudorabies Virus, John George Chosay
A Study Of Cell-Mediated Immunity During A Primary Infection With Pseudorabies Virus, John George Chosay
Masters Theses
This research defines the role and sequence of cell-mediated immune events in an in vivo virus infection. Pseudorabies virus infected CF1 mice lungs, were assessed by his to logical or immunohistochemical means. Using fluorescein isothiocyanate Tabled anti-T lymphocyte (ATG) and lissamine rhodamineB200 labeled anti-macrophage globulins (AMG), the number of lymphocytes and macrophages were determined. An early increase in T cells (four hours post-innoculation) was followed by an increase in macrophages (15 hours post-innoculation). Thereafter tissue levels of T cells and macrophages remained constant. Thymus dependent lymphocyte; failed to show increase macrophages clustering as pathology progressed. The first pathological changes in …
An Examination Of The Red Blood Cell Alloantigentic System In The Chinese Hamster, Kathryn Elliget Blashfield
An Examination Of The Red Blood Cell Alloantigentic System In The Chinese Hamster, Kathryn Elliget Blashfield
Masters Theses
This research was done to determine whether any ABH-like blood group system was present in Chinese hamsters (Cricetulus griseus). The population of animals included 35 hamsters, 20 "normal" or outbred (10 males; 10 females) and 15 diabetic from The Upjohn Company's colony of inbred diabetic animals (8 males; 7 females; BE and BA lines). Animal sera and erythrocytes were compared by: hyperimmunization of rabbits (10 New Zealand White) with hamster erythrocytes; screening hamster erythrocytes and plasmas with human blood diagnostic typing antisera (Ortho Diagnostics), anti-H lectin, anti-A1 lectin and human red cells of various types; absorption and elution of …