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Articles 1 - 6 of 6
Full-Text Articles in Life Sciences
Investigating The Role Of Rna-Binding Protein 5 In The Life Cycle Differentiation Of Trypanosoma Brucei, David Anaguano Pillajo
Investigating The Role Of Rna-Binding Protein 5 In The Life Cycle Differentiation Of Trypanosoma Brucei, David Anaguano Pillajo
Masters Theses
Trypanosomatid parasites such as Trypanosoma brucei have unusual mechanisms of gene expression including polycistronic transcription, mitochondrial RNA editing and trans-splicing. Additionally, these protists rely mainly on post-transcriptional regulation where RNA-binding proteins (RBP) have shown to play a major role. RBP6 and RBP10 are two examples of RBPs that play crucial roles in procyclic and bloodstream form parasites differentiation respectively, by post-transcriptional regulation. Over-expression of RBP6 is enough to promote differentiation into metacyclic trypomastigotes that are infective to mice. However, continuous expression is required, and this pattern does not reflect the natural expression in the tsetse fly or the influence of …
Regulation Of Expression Of The T Cell Wc1 Co-Receptor And Pattern Recognition Receptor Multigene Family, Payal Damani-Yokota
Regulation Of Expression Of The T Cell Wc1 Co-Receptor And Pattern Recognition Receptor Multigene Family, Payal Damani-Yokota
Doctoral Dissertations
WC1 molecules are hybrid PRR/co-receptors exclusively expressed on gd T cells of ruminants while humans and mice have related receptors. Bovine WC1 molecules bind pathogens directly and specifically and augment TCR-mediated signals. Using NGS, we found all 13 WC1 genes transcribed by blood gd T cells with no significant differences at birth although by adulthood the relative proportions of transcripts was somewhat altered perhaps attributable to immunological experience. WC1+ gd T cells form two main subpopulations in blood, WC1.1 and WC1.2, distinguished by reactivity with various anti-WC1 mAbs. Their WC1 molecules also differ in their ability to bind particular microbes …
Canonical Notch Signaling Positively Regulates Mir-155 Transcription In A Nfκb-Dependent Manner, Joe Torres
Canonical Notch Signaling Positively Regulates Mir-155 Transcription In A Nfκb-Dependent Manner, Joe Torres
Doctoral Dissertations
MicroRNA-155 (miR-155) is an important pro-inflammatory molecule that controls immune responses in both physiological and pathological settings. Although miR-155 is well studied, little is known about its transcriptional regulation in T cells. We sought to further understand the mechanisms by which miR-155 transcription is induced following T cell activation. We found that miR-155 transcription is dependent on the activity of the Notch and NFkB signaling pathways. It was determined that the canonical Notch signaling pathway was involved in this regulation and that it was dependent upon the activation of NFkB. Additional work confirmed that both NFkB and Notch1 directly bind …
Defining And Manipulating The Function Of Protein Kinase C-Theta In Graft Versus Host Responses, Emrah Ilker Ozay
Defining And Manipulating The Function Of Protein Kinase C-Theta In Graft Versus Host Responses, Emrah Ilker Ozay
Doctoral Dissertations
Immune-mediated tissue destruction of graft-vs-host disease (GvHD) remains a major barrier to greater use of bone marrow transplantation (BMT). It is found that alloreactive donor-derived T cells activated through their T cell receptor (TCR) are primarily the major contributors to the immunopathobiology of GvHD. Protein kinase C-theta (PKCtheta), a crucial, early downstream kinase of TCR signaling, enhances T cell activation, thereby promoting alloreactive responses such as differentiation, proliferation, migration, and cytotoxicity. Thus, delineating specific ways of interfering PKCtheta signaling is beneficial for the GvHD treatment or prevention. Here, we investigated the molecular mechanisms driven by PKCtheta in T cells by …
Polymeric Peptide Mimics For Protein Delivery, Coralie Backlund
Polymeric Peptide Mimics For Protein Delivery, Coralie Backlund
Doctoral Dissertations
The plasma membrane is a major obstacle in the development and use of biomacromolecules for intracellular applications. Consequently, proteins with intracellular targets represent an enormous, yet under studied avenue for therapeutics. Extended research has aimed at facilitating intracellular delivery of exogenous proteins using protein transduction domains (PTDs), which allow transport of bioactive molecules into cells. Synthetic polymers, inspired by PTDs, provide a well-controlled platform to vary molecular architecture for structure activity relationship studies. Specifically, this thesis focuses on the use of ring-opening metathesis, a facile and efficient polymerization technique, through which we can vary structural parameters to optimize delivery of …
Effects Of Floral Phytochemicals On Growth And Evolution Of A Parasite Of Bumble Bees, Evan Palmer-Young
Effects Of Floral Phytochemicals On Growth And Evolution Of A Parasite Of Bumble Bees, Evan Palmer-Young
Doctoral Dissertations
Background: Nectar and pollen are rich in phytochemicals, some of which can reduce disease in pollinators, including agriculturally important honey and bumble bees. Floral phytochemicals could influence the ecological and evolutionary relationships between plants, their pollinators, and parasites that cause pollinator disease. Antiparasitic effects of phytochemicals could be exploited to ameliorate pollinator disease and decline, and thereby sustain pollinator-dependent agricultural production. However, prior studies showed variable effects of phytochemicals on infection in live bees, where differences in bee genotype, abiotic conditions, and parasite strain could influence results. Approach: I used cell cultures of the intestinal trypanosome parasite of bumble bees, …