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Immunology and Infectious Disease

University of Kentucky

Theses and Dissertations--Animal and Food Sciences

Theses/Dissertations

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Monocyte Abundance And White Blood Cell Expression Of Prolactin And Nf-Kb Pathway Genes In Beef Steers Are Altered By Grazing Toxic Endophyte-Infected Tall Fescue And Lipopolysaccharide Challenge, Cecilia Winfrey Jan 2021

Monocyte Abundance And White Blood Cell Expression Of Prolactin And Nf-Kb Pathway Genes In Beef Steers Are Altered By Grazing Toxic Endophyte-Infected Tall Fescue And Lipopolysaccharide Challenge, Cecilia Winfrey

Theses and Dissertations--Animal and Food Sciences

Experiment 1 tested the hypothesis that ad libitum consumption of a 1:1 blend (MIX) of sodium selenite (ISe) and SELPLEX vs solely ISe as selenium (Se) supplements (27 ppm) in a vitamin-mineral mix (V-M) by steers subjected to summer-long grazing of toxic endophyte-infected tall fescue (TE) would ameliorate the negative effects of TE by positively affecting the expression pattern of prolactin (PRL) and NF-kB pathway genes by circulating leukocytes (WBC). Experiment 2 tested the hypothesis that the MIX form of supplemental Se also would ameliorate the negative effects of in vitro blood lipopolysaccharide challenge (LPS) on WBC gene expression. The …


Relationships Between Animal Temperament And Systemic Immune Responses In Beef Cattle Exposed To Conditions Associated With Conventional Management, Alexander W. Altman Jan 2019

Relationships Between Animal Temperament And Systemic Immune Responses In Beef Cattle Exposed To Conditions Associated With Conventional Management, Alexander W. Altman

Theses and Dissertations--Animal and Food Sciences

Measures of temperament have been shown to influence physiological responses. Exit velocity (EV) has been identified as an objective, robust measure of temperament that can be used to predict subsequent performance of cattle. Additionally, previous studies from our lab indicate this measure of temperament may be related to production of interferon-γ (IFN-γ), a cytokine associated with cell-mediated immunity (CMI). Whereas research has investigated effects of EV upon immune responses, the overall goal of these studies was to examine this relationship under a variety of scenarios including human handling, transportation, and exposure to endophyte-infected tall fescue (E+) for determination of its …


Detection Of Antibodies Against Parascaris Equorum Excretory-Secretory Antigens, Steffanie V. Burk Jan 2013

Detection Of Antibodies Against Parascaris Equorum Excretory-Secretory Antigens, Steffanie V. Burk

Theses and Dissertations--Animal and Food Sciences

Parascaris equorum is a nematode parasite that infects young horses, sometimes causing unthriftiness, respiratory signs, or intestinal impaction in severe cases. Infection can be diagnosed by detection of eggs in feces, but this is only possible after the worms are fully mature. The goal of this study was to develop an antibody-based test for prepatent diagnosis of P. equorum infection. To produce western blot (WB) antigen, P. equorum larvae were cultured for collection of excretory-secretory antigens (ESA). Sera from 18 pregnant broodmares, their subsequent foals, and a group of 12 older mares and geldings were analyzed. In order to check …