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Lps-Induced Production Of Inflammatory Mediators In The Liver Of Postnatal Animals, Valerie P. Le Rouzic

Seton Hall University Dissertations and Theses (ETDs)

Lipopolysaccharide (LPS) is the primary component of the outer membrane of Gram-negative bacteria and is responsible for the majority of inflammatory effects of infections from Gram-negative bacteria. To gain better understanding of the effects that postnatal age has on the inflammatory response, pups were randomly assigned to be treated with 250 µg/kg of LPS or saline at postnatal day (P) 1, P21, and P70. Two hours post stimulation, the pups were sacrificed and their livers were harvested for total RN A extraction. Relative mRNA levels of inflammatory genes and �-actin were determined using RT-PCR analysis with appropriate rat sense and …