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Detection Of Free Living Amoebae, Acanthamoeba And Naegleria, In Swimming Pools, Malaysia, Arine Fadzlun Ahmad, Init Ithoi, Lau Yee Ling Dec 2010

Detection Of Free Living Amoebae, Acanthamoeba And Naegleria, In Swimming Pools, Malaysia, Arine Fadzlun Ahmad, Init Ithoi, Lau Yee Ling

Arine Fadzlun Ahmad

This study reports the detection of Acanthamoeba and Naegleria species in 14 swimming pools around Petaling Jaya and Kuala Lumpur, Malaysia. Sampling was carried out at 4 sites (the platforms (P), wall (W), 1 meter from the wall (1) and middle (2)) of each swimming pool. These free living amoebae (FLA) were detected under light and inverted microscopes after being cultured on the surface of non-nutrient agar lawned with Escherichia coli. Acanthamoeba species were detected in higher number of culture plates from all sampling sites of all the swimming pools. While Naegleria, were detected in fewer culture plates at 3 …


Prevalence Of Scabies And Head Lice Among Children In A Welfare Home In Pulau Pinang, Malaysia, Rohela Mahmud Dec 2010

Prevalence Of Scabies And Head Lice Among Children In A Welfare Home In Pulau Pinang, Malaysia, Rohela Mahmud

Rohela Mahmud

This is a survey of 120 children for scabies and head lice infestations in a welfare home in Pulau Pinang. Children from this welfare home (Rumah Kanak-Kanak Taman Bakti, Kepala Batas, Pulau Pinang) were randomly selected. Majority of them were Malays (72.5%) and the rest were Indians. The infestation rates were highest in the 10-12 years age group with 46% and 70% for scabies and head lice respectively. Head lice was more commonly seen in girls (65%) than boys (29%). Scabies was more commonly seen in boys (50%) than girls (16%). Overall prevalence rate for scabies was 31% and for …


Naı¨Ve T Cells Re-Distribute To The Lungs Of Selectin Ligand Deficient Mice, Thandi M. Onami, John R. Harp Jun 2010

Naı¨Ve T Cells Re-Distribute To The Lungs Of Selectin Ligand Deficient Mice, Thandi M. Onami, John R. Harp

Thandi M. Onami

BACKGROUND: Selectin mediated tethering represents one of the earliest steps in T cell extravasation into lymph nodes via high endothelial venules and is dependent on the biosynthesis of sialyl Lewis X (sLe(x)) ligands by several glycosyltransferases, including two fucosyltransferases, fucosyltransferase-IV and -VII. Selectin mediated binding also plays a key role in T cell entry to inflamed organs. METHODOLOGY/PRINCIPAL FINDINGS: To understand how loss of selectin ligands (sLe(x)) influences T cell migration to the lung, we examined fucosyltransferase-IV and -VII double knockout (FtDKO) mice. We discovered that FtDKO mice showed significant increases (approximately 5-fold) in numbers of naïve T cells in …


Opportunities And Obstacles To The Elimination Of Malaria From Peninsular Malaysia: Knowledge, Attitudes And Practices On Malaria Among Aboriginal And Rural Communities, Rohela Mahmud May 2010

Opportunities And Obstacles To The Elimination Of Malaria From Peninsular Malaysia: Knowledge, Attitudes And Practices On Malaria Among Aboriginal And Rural Communities, Rohela Mahmud

Rohela Mahmud

Background: Despite continuous efforts by the government and private sectors, malaria is still a public health problem in rural Peninsular Malaysia. This study investigated household knowledge, attitude and practices (KAP) regarding malaria in two malaria endemic communities, forest-aboriginal and rural communities, in the Lipis district of Pahang state, Malaysia. Methods: A descriptive cross-sectional study with a semi-structured questionnaire was carried out among 100 and 123 households from forest-aboriginal and rural areas, respectively. Results: Knowledge about malaria and its transmission is significantly higher among the rural participants than the aborigines (86.2% vs 76%, p < 0.01). However, use of medicinal plants and beliefs in witchcraft and sorcery in treating febrile diseases were significantly higher among the aboriginal population (p < 0.01). There were no significant differences between the two communities in terms of the knowledge about malaria symptoms, attitudes towards its severity and practices in preventive measures against malaria by using mosquito bed nets. However, the knowledge and practice of different preventive measures to combat malaria, such as insecticide and the elimination of breeding areas, was significantly higher among the rural population than the aborigines (p < 0.001). Conclusions: Both communities were aware of malaria as a disease, but knowledge, attitudes and practices were inadequate. Providing efficient health education to people residing in malaria endemic areas would improve their understanding about malaria prevention in order to bring about the elimination of malaria from the country.


Tuberculosis: An Eight Year (2000-2007) Retrospective Study At The University Of Malaya Medical Centre (Ummc), Kuala Lumpur, Malaysia, Jamaiah Ibrahim Jan 2010

Tuberculosis: An Eight Year (2000-2007) Retrospective Study At The University Of Malaya Medical Centre (Ummc), Kuala Lumpur, Malaysia, Jamaiah Ibrahim

Jamaiah Ibrahim

This was an eight year (2000-2007) retrospective study of tuberculosis in patients admitted to the UMMC. A total of 131 cases were analyzed. Malays constituted the most cases, (43%), followed by Chinese (22%), Indians (17%) and others (18%). The majority of cases were within the 21-60 year old age group, which constituted 69.5% of the total. Males were more commonly affected (65%). Most cases were reported among Malaysians (83%). The majority of patients were unemployed (39%), followed by housewives (10%), laborers (9%), students (8%), shop assistants (7%), and other occupations (27%) The most common presenting complaints were prolonged productive cough, …


Cliff Swallows, Swallow Bugs, And West Nile Virus:, Larry Clark Jan 2010

Cliff Swallows, Swallow Bugs, And West Nile Virus:, Larry Clark

Larry Clark

The cliff swallow (Petrochelidon pyrrhonota) could play an important role in the transmission of West Nile virus (WNV) because of its breeding ecology, reservoir competence status, and potentially high natural exposure rates. Cliff swallows nest within colonies and their nests are occupied year-round by swallow bugs (Oeciacus vicarius), hematophagus ectoparasites that feed primarily on cliff swallows. These parasites are likely exposed to WNV while feeding on infectious blood of nesting cliff swallow adults and nestlings and thus, if competent vectors, could contribute to seasonal elevations in WNV transmission. In addition, swallow bugs remain within nests year-round and therefore could provide …


Prevalence Of Microsporidia In An Indigenous Orang Asli Community In Pahang, Malaysia, Tan Tian Chye Jan 2010

Prevalence Of Microsporidia In An Indigenous Orang Asli Community In Pahang, Malaysia, Tan Tian Chye

Tan Tian Chye

Microsporidia are ubiquitous parasites thought to be closely related to fungi. Their presence in the environment means that humans are frequently exposed to infection. Stool samples were collected from 151 indigenous villagers from the eastern state of Pahang in 2005. The samples were concentrated with water-ether sedimentation, stained with modified trichrome stain and examined under oil-immersion microscopy. Thirty-two specimens (21.2%) were positive for microsporidia. Microsporidia were observed as ovoid or rounded ovoid shapes measuring similar to 1 mu m, with a bright pink outline containing a central or posterior vacuole. PCR amplification with specific primers on microscopy-positive specimens amplified Encephalitozoon …