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Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
Quantitative Trait Loci Mapping Reveals Candidate Pathways Regulating Cell Cycle Duration In Plasmodium Falciparum, Heather B. Reilly Ayala, Mark A. Wacker, Geoffrey Siwo, Michael T. Ferdig
Quantitative Trait Loci Mapping Reveals Candidate Pathways Regulating Cell Cycle Duration In Plasmodium Falciparum, Heather B. Reilly Ayala, Mark A. Wacker, Geoffrey Siwo, Michael T. Ferdig
Faculty Publications - Department of Biological & Molecular Science
Background: Elevated parasite biomass in the human red blood cells can lead to increased malaria morbidity. The genes and mechanisms regulating growth and development of Plasmodium falciparum through its erythrocytic cycle are not well understood. We previously showed that strains HB3 and Dd2 diverge in their proliferation rates, and here use quantitative trait loci mapping in 34 progeny from a cross between these parent clones along with integrative bioinformatics to identify genetic loci and candidate genes that control divergences in cell cycle duration. Results: Genetic mapping of cell cycle duration revealed a four-locus genetic model, including a major genetic effect …
Quantitative Dissection Of Clone-Specific Growth Rates In Cultured Malaria Parasites, Heather B. Reilly Ayala, Hongjian Wang, John A. Steuter, Anastasia M. Marx, Michael T. Ferdig
Quantitative Dissection Of Clone-Specific Growth Rates In Cultured Malaria Parasites, Heather B. Reilly Ayala, Hongjian Wang, John A. Steuter, Anastasia M. Marx, Michael T. Ferdig
Faculty Publications - Department of Biological & Molecular Science
Measurement of parasite proliferation in cultured red blood cells underpins many facets of malaria research, from drug sensitivity assays to assessing the impact of experimentally altered genes on parasite growth, virulence, and fitness. Pioneering efforts to grow Plasmodium falciparum in cultured red blood cells revolutionized malaria research and spurred the development of semi-high throughput growth assays using radio-labeled hypoxanthine, an essential nucleic acid precursor, as a reporter of whole-cycle proliferation (Trager and Jensen, 1976; Desjardins et al., 1979). Use of hypoxanthine (Hx) and other surrogate readouts of whole-cycle proliferation remains the dominant choice in malaria research. While amenable to high-throughput …