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Full-Text Articles in Life Sciences
Lipopolysaccharide Induces And Activates The Nalp3 Inflammasome In The Liver, Michal Ganz, Timea Csak, Bharath D. Nath, Gyongyi Szabo
Lipopolysaccharide Induces And Activates The Nalp3 Inflammasome In The Liver, Michal Ganz, Timea Csak, Bharath D. Nath, Gyongyi Szabo
Gyongyi Szabo
AIM: To examine the activation of the Nalp3 inflammasome and its downstream targets following lipopolysaccharide (LPS)-induced stimulation in the liver. METHODS: Six-to-eight-week-old C57BL/6 chow fed mice were injected intraperitoneally with 0.5 mug/g bodyweight LPS and sacrificed 2, 4, 6, 18 or 24 h later. LPS-induced liver damage was confirmed by a biochemical assay to detect alanine aminotransferase (ALT) levels. To determine if LPS stimulation in the liver led to activation of the inflammasome, real-time quantitative polymerase chain reaction was used to evaluate the mRNA expression of components of the Nalp3 inflammasome. Enzyme-linked immunosorbent assays were used to determine the protein …
Il-22 Signaling Contributes To West Nile Encephalitis Pathogenesis, Fengwei Bai, Penghua Wang, Lauren A. Zenewicz, Jianfeng Dai, David Gate, Gong Cheng, Long Yang, Feng Qian, Xiaoling Yuan, Ruth R. Montgomery, Richard A. Flavell, Terrence Town, Erol Fikrig
Il-22 Signaling Contributes To West Nile Encephalitis Pathogenesis, Fengwei Bai, Penghua Wang, Lauren A. Zenewicz, Jianfeng Dai, David Gate, Gong Cheng, Long Yang, Feng Qian, Xiaoling Yuan, Ruth R. Montgomery, Richard A. Flavell, Terrence Town, Erol Fikrig
Faculty Publications
The Th17 cytokine, IL-22, regulates host immune responses to extracellular pathogens. Whether IL-22 plays a role in viral infection, however, is poorly understood. We report here that Il22-/- mice were more resistant to lethal West Nile virus (WNV) encephalitis, but had similar viral loads in the periphery compared to wild type (WT) mice. Viral loads, leukocyte infiltrates, proinflammatory cytokines and apoptotic cells in the central nervous system (CNS) of Il22-/- mice were also strikingly reduced. Further examination showed that Cxcr2, a chemokine receptor that plays a non-redundant role in mediating neutrophil migration, was significantly reduced in Il22-/- …
Role Of Il-10 In The Immune Response To Staphylococcus Aureus Nasal Carriage, Teresa Fernandez Plaza
Role Of Il-10 In The Immune Response To Staphylococcus Aureus Nasal Carriage, Teresa Fernandez Plaza
Electronic Thesis and Dissertation Repository
Staphylococcus aureus is a human pathogen with the potential to cause life-threatening diseases, but it can also be found as a commensal in up to 50% of the population. Its main reservoir is located in the nostrils, where macrophages are the principal mucosal APC and elicit a predominantly IL-10 response. Induction of IL-10 requires phosohoinositide 3-kinase activity, upon stimulation with S. aureus ligands through toll-like receptor 2 binding. It is hypothesized that IL-10 production by macrophages in the nasal mucosa allows S. aureus to persist in this niche as a commensal. To test this hypothesis, a cohort of S. aureus …
Positive Emotions And Immune Respose To Influenza In Medically Stable Older Adults, Maryann J. Gierloff
Positive Emotions And Immune Respose To Influenza In Medically Stable Older Adults, Maryann J. Gierloff
Dissertations
Influenza results in substantial human suffering and health care costs. Evidence from psychoneuroimmunology suggests that emotions influence the immune system and may alter susceptibility to infectious diseases, like influenza. The purpose of this investigation was to evaluate the influence of psychological factors, health behaviors, circulating levels of proinflammatory cytokines and the development of influenza-like illness in older adults. Medically stable participants, 55 years of age and over, were enrolled from the general community and an elderly community. Psychological factors (emotions and perceived stress), health behaviors (sleep and physical activity) and plasma cytokine levels (IL-6 and IL-1B) were evaluated with respect …