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The Development Of A Bluetongue Virus Diagnostic Assay Using Peptides Derived From The Structural Protein Vp7 And Nonstructural Protein Ns2, Yi Lu
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Several peptides derived from viral proteins, VP7 and NS2 of bluetongue virus (BTV), were synthesized for an immunologic detection assay: distinguishing between bluetongue virus infected, vaccinated, and noninfected animals (ruminants) with dot blot analysis. The School of Veterinary Medicine, University of California, Davis, supplied the serum samples from 24 bovine, 18 caprine, 23 ovine. Another 205 bison serum samples were obtained in Utah. The serum samples of BTV-infected animals revealed detectable antibodies to both VP7 and NS2 peptides, whereas vaccinated animals only exhibited antibodies against VP7. Noninfected animals did not display antibody against either viral peptide. These data confirmed the …