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Life Sciences Commons

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Genetics and Genomics

1985

Theses/Dissertations

Articles 1 - 2 of 2

Full-Text Articles in Life Sciences

The Use Of Motor Coordination Endpoints In The F1 Generation To Evaluate For Effects Of Ethylmethanesulfonate On Pre- And Post-Meiotic Sperm Cells Of Male Mice, Lee Goldner Dec 1985

The Use Of Motor Coordination Endpoints In The F1 Generation To Evaluate For Effects Of Ethylmethanesulfonate On Pre- And Post-Meiotic Sperm Cells Of Male Mice, Lee Goldner

Masters Theses

Groups of 5 male HA(ICR) mice were injected intraperitoneally with 60, 150, 300, or 600 mg/kg body weight of ethylmethanesulfonate or with a saline control. Each male was mated to two untreated females at two weeks after treatment and then again at five weeks after treatment. The treatment groups were labelled post-meiotic and pre-meiotic relative to the stage of spermatogenesis at time of treatment. Progeny were evaluated by observations of litter size, body weight, negative geotactic response, swimming patterns, limb use, water escape time and open field motor coordination activity. Body weight, geotactic response, limb use and open field motor …


A Comparison Of The Acidic Extracellular Polysaccharides From Rhizobium Trifolii Mutants Affected In Trifolium Repens Root Hair Curling, Brian Andrew Hanley Jan 1985

A Comparison Of The Acidic Extracellular Polysaccharides From Rhizobium Trifolii Mutants Affected In Trifolium Repens Root Hair Curling, Brian Andrew Hanley

Masters Theses

The compositions and sugar linkages of the acidic extracellular polysaccharide (EPS) from several Rhizobium trifolii mutants were compared. These mutants were derived from a wild type Rhizobium trifolii, ANU843, and are unable to cause normal root hair curling, Hac-, or nodulate clover, Nod-. Three of the mutants contain a Tn5 insertion in a 5.3 kb BglII fragment of the symbiotic plasmid (pSym). A fourth mutant is missing the pSym, which includes much of the 5.3 kb BglII fragment, both of these deletions result in Hac-Nod- phenotypes. When the 5.3 kb BglII fragment is cloned (pRT150) and reinserted into the …