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Localization Of Two New Isozyme Loci, Hexl And Tpi4, To Chromosome 3, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman Jan 1985

Localization Of Two New Isozyme Loci, Hexl And Tpi4, To Chromosome 3, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman

Jonathan F. Wendel

Recent work with starch gel electrophoresis of coleoptile extracts has indicated that Hexl, the structural locus for the most anodal set of hexokinase isozymes, and Tpi4, which encodes the slowest migrating set of triose phosphate isomerase bands, are located on chromosome 3.


Mapping Data For 34 Isozyme Loci Currently Being Studied, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman Jan 1985

Mapping Data For 34 Isozyme Loci Currently Being Studied, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman

Jonathan F. Wendel

With the recent localization of several new loci, a composite listing of these loci and chromosomal locations has been compiled and is shown in Table 1. Although locations of some of the loci are tentative, many are very precisely located from studies involving 1600 to more than 1900 F2 plants.


Linkage Relationships Between A New Locus, Hex2, And Previously Assigned Loci On Chromosome 6, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman Jan 1985

Linkage Relationships Between A New Locus, Hex2, And Previously Assigned Loci On Chromosome 6, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman

Jonathan F. Wendel

Starch gel electrophoresis of maize coleoptile extracts in our laboratory has shown two zones of staining for isozymes of hexokinase. Genetic analyses 9 indicate that the slower migrating set of bands is encoded by the structural gene Hex2 and that the enzyme is monomeric. Recent linkage analyses and work with B-A translocation stocks (generously supplied by Jack Beckett) have allowed for the placement ofHex2 on the long arm of chromosome 6. Its location relative to other markers on 6L was determined from the analysis of two very large F2 populations generated for other reasons.


Twelve New Isozyme Loci In Maize: Progress Report On Chromosomal Locations, The Subunit Composition, And Subcellular Localization Of Their Products, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman Jan 1985

Twelve New Isozyme Loci In Maize: Progress Report On Chromosomal Locations, The Subunit Composition, And Subcellular Localization Of Their Products, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman

Jonathan F. Wendel

To improve the degree to which the com genome is biochemically marked, we have expanded the number of enzyme systems analyzed by starch gel electrophoresis. Seven new enzyme systems encoded by a minimum of 16 loci have been added to the 23 loci used for previous work in our laboratory. A wide range of domestic and exotic maize has been screened for variation at these new loci and genetic analysis of variant electromorphs is well under way. Crosses with B-A translocation stocks (kindly provided by Jack Beckett), previously mapped allozyme loci, and in a few cases morphological markers, are being …