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Full-Text Articles in Life Sciences
The Functions Of Animal Micrornas., Victor Ambros
The Functions Of Animal Micrornas., Victor Ambros
Victor R. Ambros
MicroRNAs (miRNAs) are small RNAs that regulate the expression of complementary messenger RNAs. Hundreds of miRNA genes have been found in diverse animals, and many of these are phylogenetically conserved. With miRNA roles identified in developmental timing, cell death, cell proliferation, haematopoiesis and patterning of the nervous system, evidence is mounting that animal miRNAs are more numerous, and their regulatory impact more pervasive, than was previously suspected.
The C. Elegans Heterochronic Gene Lin-46 Affects Developmental Timing At Two Larval Stages And Encodes A Relative Of The Scaffolding Protein Gephyrin., Anita Pepper, Jill Mccane, Kevin Kemper, Denise Yeung, Rosalind Lee, Victor Ambros, Eric Moss
The C. Elegans Heterochronic Gene Lin-46 Affects Developmental Timing At Two Larval Stages And Encodes A Relative Of The Scaffolding Protein Gephyrin., Anita Pepper, Jill Mccane, Kevin Kemper, Denise Yeung, Rosalind Lee, Victor Ambros, Eric Moss
Victor R. Ambros
The succession of developmental events in the C. elegans larva is governed by the heterochronic genes. When mutated, these genes cause either precocious or retarded developmental phenotypes, in which stage-specific patterns of cell division and differentiation are either skipped or reiterated, respectively. We identified a new heterochronic gene, lin-46, from mutations that suppress the precocious phenotypes caused by mutations in the heterochronic genes lin-14 and lin-28. lin-46 mutants on their own display retarded phenotypes in which cell division patterns are reiterated and differentiation is prevented in certain cell lineages. Our analysis indicates that lin-46 acts at a step immediately downstream …
Expression Profiling Of Mammalian Micrornas Uncovers A Subset Of Brain-Expressed Micrornas With Possible Roles In Murine And Human Neuronal Differentiation., Lorenzo F. Sempere, Sarah Freemantle, Ian Pitha-Rowe, Eric Moss, Ethan Dmitrovsky, Victor R. Ambros
Expression Profiling Of Mammalian Micrornas Uncovers A Subset Of Brain-Expressed Micrornas With Possible Roles In Murine And Human Neuronal Differentiation., Lorenzo F. Sempere, Sarah Freemantle, Ian Pitha-Rowe, Eric Moss, Ethan Dmitrovsky, Victor R. Ambros
Victor R. Ambros
Background The microRNAs (miRNAs) are an extensive class of small noncoding RNAs (18 to 25 nucleotides) with probable roles in the regulation of gene expression. In Caenorhabditis elegans, lin-4 and let-7 miRNAs control the timing of fate specification of neuronal and hypodermal cells during larval development. lin-4, let-7 and other miRNA genes are conserved in mammals, and their potential functions in mammalian development are under active study. Results In order to identify mammalian miRNAs that might function in development, we characterized the expression of 119 previously reported miRNAs in adult organs from mouse and human using northern blot analysis. Of …
A Short History Of A Short Rna, Victor Ambros, Rosalind Lee, Rhonda Feinbaum
A Short History Of A Short Rna, Victor Ambros, Rosalind Lee, Rhonda Feinbaum
Victor R. Ambros
Comment on: The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. [Cell. 1993]
Identification Of Micrornas And Other Tiny Noncoding Rnas By Cdna Cloning, Victor Ambros, Rosalind Lee
Identification Of Micrornas And Other Tiny Noncoding Rnas By Cdna Cloning, Victor Ambros, Rosalind Lee
Victor R. Ambros
MicroRNAs (miRNAs) and other small RNAs can be identified by cloning and sequencing cDNAs prepared from the ∼22-nt fraction of total RNA. Methods are described for the construction of cDNA libraries from small noncoding RNAs through the use of T4 RNA ligase, reverse transcriptase, and polymerase chain reaction. cDNAs are cloned in λ or plasmid vectors, and the sequences are compared to annotated genomic sequence databases, and analyzed by RNA folding programs to distinguish miRNA sequences from other small RNAs of similar size. Northern blot hybridization is used to confirm the expression of small RNAs in vivo.