Life Sciences Commons^™

Comparison Of Electron And Light Microscopy Of Chromosome Aberration Induced In Human Lymphocytes By Adriamycin And Mitomycin C, Abbas Parsian

Dissertations

The present study was designed to develop a technique to prepare human chromosomes for sequential light and electron microscopic observation and to compare detectability of chromosome aberrations induced by adriamycin and mitomycin C by the two procedures. The technique developed preserved the morphological and structural organization of chromosome while allowing observation of the cell's entire chromosome complement. It was rapid and reproducible and chromosomes could be treated and stained for banding.

Light microscopic data showed that in cultures of human lymphocytes both drugs induce chromosome aberrations. In comparison with controls both drugs produced significantly more chromosome and chromatid fragments. Electron …

The Effects Of Strain Differences On The Binding Of Concavalian A To Mouse Sperm, Lisa A. Mckay

Honors Theses

The current study, lectins were used as genetic probes to determine the phenotype of the sperm from different strains of mice. Lectins are proteins of non-immune origin, which bind to specific carbohydrates (McCoy, 1986). Being that virtually all cell membranes contain carbohydrate components, lectins have been used to identify all types of cells. In this study, a biotinylated lectin Concavalian A (Con A) derived from Canavalia ensiformis, was used and binding was detected using an avidin-biotin peroxidase (ABP) complex technique. The object of the current research was to 1) determine if mouse strain differences have an effect on lectin binding, …

Strain Differences And Mutagen Treatment Influence Lectin Binding To Mouse Germ Cells, Marilyn Schmalz Holm

Masters Theses

Carbohydrate moieties on mouse germ cell surfaces were characterized by investigating the binding of FITC-labeled lectins to sperm and testis cells. Differences in lectin binding between different mouse strains, including T/t mice, were found. However, there was no evidence for the involvement of sialic acid or L-fucose in the T/t locus antigens. Antiserum to the t⁰, t⁶ haplotype was equally reactive to both ICR and T/t mouse testis cells in both immunofluorescence and cytotoxicity assays. Trypsinization of testis cells increased binding of soybean agglutinin and decreased binding of wheat germ agglutinin.

A method was developed for flow …