Life Sciences Commons^™

Expression Of Insulin Responsive Genes In Insulin Resistant Conditions, And The Effect Of Selenium On Gene Expression, David L. Ruff

Masters Theses

Chronically high blood glucose levels lead to many problems, such as insulin resistance, the hallmark of Type II diabetes. Increased flux through the hexosamine biosynthesis pathway is one mechanism by which high glucose as well as glucosamine has been shown to induce insulin resistance. This study tests the effects of glucosamine induced insulin resistance on insulin regulation of the metabolic genes glucose-6-phosphate dehydrogenase (G6PDH) and fatty acid synthase (FAS) as well as insulin responsive proteins tribbles homolog (TRIB3) and sterol regulatory element binding protein (SERBP-1c) 1c.

Selenium, a micronutrient has been shown to be an effective insulin mimetic in Type …

Studies On Enzyme Functional Evolution In The Sabath Multigene Family Using Phylogenetic And Biochemical Approaches, Ruiqi Huang

Masters Theses

Gene duplication is believed to be the major source for providing genetic materials for the innovation and diversification of protein functions; natural selection and/or neutral drift then works on these genetic materials to guide their evolutionary directions. Here, I used the salicylic acid/benzoic acid/theobromine (SABATH) multigene family to study how natural selection acted on duplicated genes to prompt functional diversification. Members in this family methylate plant secondary metabolites by transferring the methyl group from S-adenosyl-L-methionine (SAM) to the carboxyl group or ring nitrogen of the substrates. In the Apocynaceae and Solanaceae lineage of this family, I documented three putative gene …

Cloning And Sequencing Of The Eco Rii Monomer Fragment Of Satellite Dna From The Mouse Mus Musculus, Timothy Patrick Boyle

Masters Theses

Total mouse satellite DNA was isolated in cesium chloride gradients in the presence of the dye Hoechst 33258. This satellite fraction was digested with Bst Nl, an isoschizomer of the restriction enzyme Eco RII, and the 234 base pair monomer fragment was cloned into the plasmid vector pBS. Dideoxynucleotide sequencing of the cloned DNA yielded significant homology with the published consensus sequence of mouse satellite DNA (92%).

Strain Differences And Mutagen Treatment Influence Lectin Binding To Mouse Germ Cells, Marilyn Schmalz Holm

Masters Theses

Carbohydrate moieties on mouse germ cell surfaces were characterized by investigating the binding of FITC-labeled lectins to sperm and testis cells. Differences in lectin binding between different mouse strains, including T/t mice, were found. However, there was no evidence for the involvement of sialic acid or L-fucose in the T/t locus antigens. Antiserum to the t⁰, t⁶ haplotype was equally reactive to both ICR and T/t mouse testis cells in both immunofluorescence and cytotoxicity assays. Trypsinization of testis cells increased binding of soybean agglutinin and decreased binding of wheat germ agglutinin.

A method was developed for flow …

The Use Of Motor Coordination Endpoints In The F1 Generation To Evaluate For Effects Of Ethylmethanesulfonate On Pre- And Post-Meiotic Sperm Cells Of Male Mice, Lee Goldner

Masters Theses

Groups of 5 male HA(ICR) mice were injected intraperitoneally with 60, 150, 300, or 600 mg/kg body weight of ethylmethanesulfonate or with a saline control. Each male was mated to two untreated females at two weeks after treatment and then again at five weeks after treatment. The treatment groups were labelled post-meiotic and pre-meiotic relative to the stage of spermatogenesis at time of treatment. Progeny were evaluated by observations of litter size, body weight, negative geotactic response, swimming patterns, limb use, water escape time and open field motor coordination activity. Body weight, geotactic response, limb use and open field motor …

Analysis Of Single Sperm Acrosomal Enzymes In Genetic Toxicology, Steven Craig Johnson

Masters Theses

No abstract provided.

The Prevalence Of 47, Xyy Males Among Collegiate Basketball Players, Joy Ann Price

Masters Theses

No abstract provided.

Relationship Of Chemical Structure And Mutagenic Activity Of Streptozotocin And Its Analogs, Riaz-Ul Islam Zuberi

Masters Theses

No abstract provided.

N-Methyl-N’-Nitro-N-Nitrosoguanidine Induced Dominant And Recessive Mutations In Zea Mays, Frank Charles Janca

Masters Theses

No abstract provided.

A Review Of The Status Of The Teaching Of Genetics And Evolution In High School Biology Courses With Suggestions For Improving The Teaching Of These Subjects, Carl Jay Bajema

Masters Theses

Introduction

It is the purpose of this thesis to: (1) discuss the place of genetics and evolution in high school biology courses; (2) specify the objectives that should be attained in teaching genetics and evolution; (3) discuss the current status of the teaching of genetics and evolution in high school biology courses; (4) suggest means of overcoming the three problems which have resulted in the poor teaching of genetics and evolution on the high school level -- first, the problem of religious opposition to the teaching of evolution; second, the problem of a lack of knowledge on the part of …