Life Sciences Commons^™

A Deep Learníng-Based Data Minimization Algorithm For Big Genomics Data In Support Of Lot And Secure Smart Health Services, Mohammed Aledhari

Dissertations

In the age of Big Genomics Data, institutes such as the National Human Genome Research Institute (NHGRI),1000-Genomes project, and the international cancer sequencing consortium are faced with the challenge of sharing large volumes of data between internationallydispersed sample collectors, data analyzers, and researchers, a process that up until now has been plagued by unreliable transfers and slow connection speeds. These occur due to the inherent throughput bottlenecks of traditional transfer technologies. One suggested solution is using the cloud as an infrastructure to solve the store and analysis challenges. However, the transfer and share of the genomics datasets between biological laboratories …

Inbreeding, Male Diploidy, And Complementary Sex Determination In The Solitary Wasp Euodynerus Foraminatus, Julie Kozaczka Stahlhut

Dissertations

In the insect order Hymenoptera, which includes wasps, bees, and ants, females normally develop from fertilized eggs and are diploid, while males develop from unfertilized eggs and are haploid. Underlying mechanisms of sex determination are more complex and vary among different hymenopteran taxa. One such method is single-locus complementary sex determination, or sl-CSD, in which sex is determined by a single gene locus with many alleles. In species with sl-CSD, diploid individuals that are heterozygous at the sex locus develop as normal females and hemizygous (haploid) individuals as normal males, but diploids homozygous at the sex locus will also develop …

An Account Of Expert Phylogenetic Tree Construction From The Problem-Solving Research Tradition In Science Education, Steven D. Brewer

Dissertations

Content knowledge, strategic knowledge, and forms of knowledge organization used in phylogenetic tree construction are described to account for expert performance. A literature review is used to situate this research with respect to studies of: (a) the teaching of evolution, (b) problem-solving expertise, (c) physics and biology problem solving, and (d) the practice of phylogenetic systematics. Rationales are provided for the development of a computer-based problem-solving environment, model problems, and research problems and methodology.

Each problem consisted of a matrix of coded and polarized phylogenetic data organized by taxa and characters. Four series of research problems were constructed that varied …

Comparison Of Electron And Light Microscopy Of Chromosome Aberration Induced In Human Lymphocytes By Adriamycin And Mitomycin C, Abbas Parsian

Dissertations

The present study was designed to develop a technique to prepare human chromosomes for sequential light and electron microscopic observation and to compare detectability of chromosome aberrations induced by adriamycin and mitomycin C by the two procedures. The technique developed preserved the morphological and structural organization of chromosome while allowing observation of the cell's entire chromosome complement. It was rapid and reproducible and chromosomes could be treated and stained for banding.

Light microscopic data showed that in cultures of human lymphocytes both drugs induce chromosome aberrations. In comparison with controls both drugs produced significantly more chromosome and chromatid fragments. Electron …

Standardization Of The Sister Chromatid Exchange Technique In Human Lumphocytes For Mutagen And Carcinogen Testing, Kamlesh Sharma

Dissertations

The differential staining of chromosomes with the use of 5-bromo-2'-deoxyuridine (BUdR) was discovered in 1972. Since then, the BUdR technique has been modified and improved. It is used to study cellular kinetics; yet its most extensive use today is focused on studying sister chromatid exchange (SCE). Studies show that the frequency of SCEs increases significantly in the presence of mutagenic and carcinogenic agents. Hence, the BUdR technique (commonly referred to as the SCE technique) has been useful as a diagnostic tool to test for mutagenic anc carcinogenic agents.

Investigators have used differential staining to obtain a baseline incidence of SCEs …