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Reference Gene Selection For Rt-Qpcr Analysis In Harmonia Axyridis, A Global Invasive Lady Beetle, Xiaowei Yang, Huipeng Pan, Ling Yuan, Xuguo Zhou

Entomology Faculty Publications

Harmonia axyridis is a voracious predator, a biological control agent, and one of the world most invasive insect species. The advent of next-generation sequencing platforms has propelled entomological research into the genomics and post-genomics era. Real-time quantitative PCR (RT-qPCR), a primary tool for gene expression analysis, is a core technique governs the genomic research. The selection of internal reference genes, however, can significantly impact the interpretation of RT-qPCR results. The overall goal of this study is to identify the reference genes in the highly invasive H. axyridis. Our central hypothesis is that the suitable reference genes for RT-qPCR analysis …