Life Sciences Commons^™

Impact Of An External Energy On Staphylococcus Epidermis [Atcc –13518] In Relation To Antibiotic Susceptibility And Biochemical Reactions – An Experimental Study, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Purpose:

While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiment on Staphylococcus epidermis [ATCC –13518], validate the effects of such energy transmitted through a person, Mahendra Trivedi, which has produced an impact measurable in scientifically rigorous manner.

Methods:

Staphylococcus epidermis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mahendra Trivedi to the sealed tubes containing strain, the process taking about 3 minutes and were analyzed within 10 …

Gene Alterations By Peroxisome Proliferator-Activated Receptor Gamma Agonists In Human Colorectal Cancer Cells, Maria Cekanova, J Yuan, X Li, K B. Kim, Seung J. Baek

Maria Cekanova MS, RNDr, PhD

The peroxisome proliferator-activated receptor gamma (PPARgamma) is a nuclear transcription factor that controls the genes involved in metabolism and carcinogenesis. In the present study, we examined the alteration of gene expression in HCT-116 human colorectal cancer cells by PPARgamma agonists: MCC-555 (5 microM), rosiglitazone (5 microM), and 15-deoxy-Delta12,14-prostaglandin J2 (1 microM). The long-oligo microarray data revealed a list of target genes commonly induced (307 genes) and repressed (32 genes) by tested PPARgamma agonists. These genes were analyzed by Onto-Express software and KEGG pathway analysis and revealed that PPARgamma agonists are involved in cell proliferation, focal adhesion, and several signaling pathways. …

Microproteomics: Analysis Of Protein Diversity In Small Samples, Howard B. Gutstein, Jeffrey S. Morris, Suresh P. Annangudi, Jonathan V. Sweedler

Jeffrey S. Morris

Proteomics, the large-scale study of protein expression in organisms, offers the potential to evaluate global changes in protein expression and their post-translational modifications that take place in response to normal or pathological stimuli. One challenge has been the requirement for substantial amounts of tissue in order to perform comprehensive proteomic characterization. In heterogeneous tissues, such as brain, this has limited the application of proteomic methodologies. Efforts to adapt standard methods of tissue sampling, protein extraction, arraying, and identification are reviewed, with an emphasis on those appropriate to smaller samples ranging in size from several microliters down to single cells. The …

Analogy Of Issr And Rapd Markers For Comparative Analysis Of Genetic Diversity Among Different Jatropha Curcas Genotypes, Shailesh K. Tiwari Dr.

Shailesh K Tiwari Dr.

The phylogenetic relationships of 13 Jatropha genotypes from different parts of the India were analysed using 34 polymerase chain reaction (PCR) markers (20 random amplified polymorphic DNAs (RAPDs) and 14 inter simple sequence repeats (ISSRs)). Amplification of genomic DNA of the 13 genotypes, using RAPD analysis, yielded 107 fragments that could be scored, of which 91 were polymorphic, with an average of 4.55 polymorphic fragments per primer. Number of amplified fragments ranged from one (OPA20, OPB19, OPD13) to nine (OPA18) and which varied in size from 200 to 2,500 bp. Percentage of polymorphism ranged from 40% (OPB18) to a maximum …

Using The Estimated Penetrances To Determine The Range Of The Underlying Genetic Model In Case-Control Design, Mark J. Meyer, Neal Jeffries, Gang Zheng

Mark J Meyer

It is well known that the penetrance cannot be estimated using the retrospective case- control samples without making additional assumptions. In the literature the estimation of the penetrance is based on the assumptions that either the disease is rare or the disease prevalence is known. We propose an alternative approach to estimate the penetrance by assuming an underlying genetic model even though it is unknown. With this assumption, we can obtain the point estimates of the penetrances as functions of the genetic model, from which the range of underlying genetic models can be determined. We examine the performance of our …

An Update On The Diversity Of Wolbachia In Spalangia Spp. (Hymneoptera: Pteromalidae)., David B. Taylor

David B Taylor

Results from 13 additional host populations improves resolution on the diversity of Wolbachia bacteria in Spalangia spp. (Hymenoptera: Pteromalidae). These bacteria are of interest because they can profoundly affect their host’s reproduction. Manipulating Wolbachia infections may provide a method to improve the efficacy of biocontrol agents including Spalangia spp.