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Enhancing Genome Investigations In The Mosquito Culex Quinquefasciatus Via Bac Library Construction And Characterization, Paul V. Hickner, Becky Debruyn, Diane D. Lovin, Akio Mori, Christopher Saski, David W. Severson
Enhancing Genome Investigations In The Mosquito Culex Quinquefasciatus Via Bac Library Construction And Characterization, Paul V. Hickner, Becky Debruyn, Diane D. Lovin, Akio Mori, Christopher Saski, David W. Severson
Publications
Background
Culex quinquefasciatus (Say) is a major species in the Culex pipiens complex and an important vector for several human pathogens including West Nile virus and parasitic filarial nematodes causing lymphatic filariasis. It is common throughout tropical and subtropical regions and is among the most geographically widespread mosquito species. Although the complete genome sequence is now available, additional genomic tools are needed to improve the sequence assembly.
Findings
We constructed a bacterial artificial chromosome (BAC) library using the pIndigoBAC536 vector and HindIII partially digested DNA isolated from Cx. quinquefasciatus pupae, Johannesburg strain (NDJ). Insert size was estimated by Not …