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- Heat shock genes (6)
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- Gene duplication (3)
- Saccharomyces cerevisiae (3)
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- Convergent Evolution (1)
- Dicerandra frutescens (1)
- ERVII (1)
- ESP (1)
- Eryngium cuneifolium (1)
- Exocrine-gland secreting peptides (1)
- Florida scrub plants (1)
- Gener regulation (1)
- Genetic variation (1)
- H3 acetylation (1)
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Articles 1 - 19 of 19
Full-Text Articles in Life Sciences
Nucleosome Distortion As A Possible Mechanism Of Transcription Activation Domain Function, Tamara Y. Erkina, Alexandre M. Erkine
Nucleosome Distortion As A Possible Mechanism Of Transcription Activation Domain Function, Tamara Y. Erkina, Alexandre M. Erkine
Scholarship and Professional Work – COPHS
After more than three decades since the discovery of transcription activation domains (ADs) in gene-specific activators, the mechanism of their function remains enigmatic. The widely accepted model of direct recruitment by ADs of co-activators and basal transcriptional machinery components, however, is not always compatible with the short size yet very high degree of sequence randomness and intrinsic structural disorder of natural and synthetic ADs. In this review, we formulate the basis for an alternative and complementary model, whereby sequence randomness and intrinsic structural disorder of ADs are necessary for transient distorting interactions with promoter nucleosomes, triggering promoter nucleosome translocation and …
Comparative Proteomics Of Mouse Tears And Saliva: Evidence From Large Protein Families For Functional Adaptation, Robert C. Karn, Christina M. Laukaitis
Comparative Proteomics Of Mouse Tears And Saliva: Evidence From Large Protein Families For Functional Adaptation, Robert C. Karn, Christina M. Laukaitis
Scholarship and Professional Work - LAS
We produced a tear proteome of the genome mouse, C57BL/6, that contained 139 different protein identifications: 110 from a two-dimensional (2D) gel with subsequent trypsin digestion, 19 from a one-dimensional (1D) gel with subsequent trypsin digestion and ten from a 1D gel with subsequent Asp-N digestion. We compared this tear proteome with a C57BL/6 mouse saliva proteome produced previously. Sixteen of the 139 tear proteins are shared between the two proteomes, including six proteins that combat microbial growth. Among the 123 other tear proteins, were members of four large protein families that have no counterparts in humans: Androgen-binding proteins (ABPs) …
Did Androgen-Binding Protein Paralogs Undergo Neo- And/Or Subfunctionalization As The Abp Gene Region Expanded In The Mouse Genome?, Robert C. Karn, Amanda G. Chung, Christina M. Laukaitis
Did Androgen-Binding Protein Paralogs Undergo Neo- And/Or Subfunctionalization As The Abp Gene Region Expanded In The Mouse Genome?, Robert C. Karn, Amanda G. Chung, Christina M. Laukaitis
Scholarship and Professional Work - LAS
The Androgen-binding protein (Abp) region of the mouse genome contains 30 Abpa genes encoding alpha subunits and 34 Abpbg genes encoding betagamma subunits, their products forming dimers composed of an alpha and a betagamma subunit. We endeavored to determine how many Abp genes are expressed as proteins in tears and saliva, and as transcripts in the exocrine glands producing them. Using standard PCR, we amplified Abp transcripts from cDNA libraries of C57BL/6 mice and found fifteen Abp gene transcripts in the lacrimal gland and five in the submandibular gland. Proteomic analyses identified proteins corresponding to eleven of the …
The Role Of Retrotransposons In Gene Family Expansions: Insights From The Mouse Abp Gene Family, Václav Janoušek, Robert C. Karn, Christina M. Laukaitis
The Role Of Retrotransposons In Gene Family Expansions: Insights From The Mouse Abp Gene Family, Václav Janoušek, Robert C. Karn, Christina M. Laukaitis
Scholarship and Professional Work - LAS
Background: Retrotransposons have been suggested to provide a substrate for non-allelic homologous recombination (NAHR) and thereby promote gene family expansion. Their precise role, however, is controversial. Here we ask whether retrotransposons contributed to the recent expansions of the Androgen-binding protein (Abp) gene families that occurred independently in the mouse and rat genomes. Results: Using dot plot analysis, we found that the most recent duplication in the Abp region of the mouse genome is flanked by L1Md_T elements. Analysis of the sequence of these elements revealed breakpoints that are the relicts of the recombination that caused the duplication, confirming that the …
Congenic Strain Analysis Reveals Genes That Are Rapidly Evolving Components Of A Prezygotic Isolation Mechanism Mediating Incipient Reinforcement, Christina M. Laukaitis, Corina Mauss, Robert C. Karn
Congenic Strain Analysis Reveals Genes That Are Rapidly Evolving Components Of A Prezygotic Isolation Mechanism Mediating Incipient Reinforcement, Christina M. Laukaitis, Corina Mauss, Robert C. Karn
Scholarship and Professional Work - LAS
Two decades ago, we developed a congenic strain of Mus musculus, called b-congenic, by replacing the androgen-binding protein Abpa27a allele in the C3H/HeJ genome with the Abpa27b allele from DBA/2J. We and other researchers used this b-congenic strain and its C3H counterpart, the a-congenic strain, to test the hypothesis that, given the choice between signals from two strains with different a27 alleles on the same genetic background, test subjects would prefer the homosubspecific one. It was our purpose in undertaking this study to characterize the segment transferred from DBA to the C3H background in producing the b-congenic …
The Roles Of Gene Duplication, Gene Conversion And Positive Selection In Rodent Esp And Mup Pheromone Gene Families With Comparison To The Abp Family, Robert C. Karn, Christina M. Laukaitis
The Roles Of Gene Duplication, Gene Conversion And Positive Selection In Rodent Esp And Mup Pheromone Gene Families With Comparison To The Abp Family, Robert C. Karn, Christina M. Laukaitis
Scholarship and Professional Work - LAS
Three proteinaceous pheromone families, the androgen-binding proteins (ABPs), the exocrine-gland secreting peptides (ESPs) and the major urinary proteins (MUPs) are encoded by large gene families in the genomes of Mus musculus and Rattus norvegicus. We studied the evolutionary histories of the Mup and Esp genes and compared them with what is known about the Abp genes. Apparently gene conversion has played little if any role in the expansion of the mouse Class A and Class B Mup genes and pseudogenes, and the rat Mups. By contrast, we found evidence of extensive gene conversion in many Esp genes although …
Positive Selection Shaped The Convergent Evolution Of Independently Expanded Kallikrein Subfamilies Expressed In Mouse And Rat Saliva Proteomes, Robert C. Karn, Christina M. Laukaitis
Positive Selection Shaped The Convergent Evolution Of Independently Expanded Kallikrein Subfamilies Expressed In Mouse And Rat Saliva Proteomes, Robert C. Karn, Christina M. Laukaitis
Scholarship and Professional Work - LAS
We performed proteomics studies of salivas from the genome mouse (C57BL/6 strain) and the genome rat (BN/SsNHsd/Mcwi strain). Our goal was to identify salivary proteins with one or more of three characteristics that may indicate that they have been involved in adaptation: 1) rapid expansion of their gene families; 2) footprints of positive selection; and/or 3) sex-limited expression. The results of our proteomics studies allow direct comparison of the proteins expressed and their levels between the sexes of the two rodent species. Twelve members of the Mus musculus species-specific kallikrein subfamily Klk1b showed sex-limited expression in the mouse saliva proteomes. …
Genetic Variation In Past And Current Landscapes: Conservation Implications Based On Six Endemic Florida Scrub Plants, Eric S. Menges, Rebecca W. Dolan, Robert Pickert, Rebecca Yahr, Doria R. Gordon
Genetic Variation In Past And Current Landscapes: Conservation Implications Based On Six Endemic Florida Scrub Plants, Eric S. Menges, Rebecca W. Dolan, Robert Pickert, Rebecca Yahr, Doria R. Gordon
Scholarship and Professional Work - LAS
If genetic variation is often positively correlated with population sizes and the presence of nearby populations and suitable habitats, landscape proxies could inform conservation decisions without genetic analyses. For six Florida scrub endemic plants (Dicerandra frutescens, Eryngium cuneifolium, Hypericum cumulicola, Liatris ohlingerae, Nolina brittoniana, and Warea carteri), we relate two measures of genetic variation, expected heterozygosity and alleles per polymorphic locus (APL), to population size and landscape variables. Presettlement areas were estimated based on soil preferences and GIS soils maps. Four species showed no genetic patterns related to population or landscape factors. The …
A Candidate Subspecies Discrimination System Involving A Vomeronasal Receptor Gene With Different Alleles Fixed In M. M. Domesticus And M. M. Musculus, Robert C. Karn, Janet M. Young, Christina M. Laukaitis
A Candidate Subspecies Discrimination System Involving A Vomeronasal Receptor Gene With Different Alleles Fixed In M. M. Domesticus And M. M. Musculus, Robert C. Karn, Janet M. Young, Christina M. Laukaitis
Scholarship and Professional Work - LAS
Assortative mating, a potentially efficient prezygotic reproductive barrier, may prevent loss of genetic potential by avoiding the production of unfit hybrids (i.e., because of hybrid infertility or hybrid breakdown) that occur at regions of secondary contact between incipient species. In the case of the mouse hybrid zone, where two subspecies of Mus musculus (M. m. domesticus and M. m. musculus) meet and exchange genes to a limited extent, assortative mating requires a means of subspecies recognition. We based the work reported here on the hypothesis that, if there is a pheromone sufficiently diverged between M. m. domesticus and …
Functional Interplay Between Chromatin Remodeling Complexes Rsc, Swi/Snf And Iswi In Regulation Of Yeast Heat Shock Genes, Tamara Y. Erkina, Y. Zou, S. Freeling, V. I. Vorobyev, Alexander M. Erkine
Functional Interplay Between Chromatin Remodeling Complexes Rsc, Swi/Snf And Iswi In Regulation Of Yeast Heat Shock Genes, Tamara Y. Erkina, Y. Zou, S. Freeling, V. I. Vorobyev, Alexander M. Erkine
Scholarship and Professional Work – COPHS
Chromatin remodeling is an essential part of transcription initiation. We show that at heat shock gene promoters functional interactions between individual ATP-dependent chromatin remodeling complexes play critical role in both nucleosome displacement and Pol II recruitment. Using HSP12, HSP82 and SSA4 gene promoters as reporters, we demonstrated that while inactivation of SNF2, a critical ATPase of the SWI/SNF complex, primarily affects the HSP12 promoter, depletion of STH1- a SNF2 homolog from the RSC complex reduces histone displacement and abolishes the Pol II recruitment at all three promoters. From these results, we conclude that redundancy between SWI/SNF and RSC complexes …
The Mechanism Of Expansion And The Volatility It Created In Three Pheromone Gene Clusters In The Mouse (Mus Musculus) Genome, Robert C. Karn, Christina M. Laukaitis
The Mechanism Of Expansion And The Volatility It Created In Three Pheromone Gene Clusters In The Mouse (Mus Musculus) Genome, Robert C. Karn, Christina M. Laukaitis
Scholarship and Professional Work - LAS
Three families of proteinaceous pheromones have been described in the house mouse: androgen-binding proteins (ABPs), exocrine gland–secreting peptides (ESPs), and major urinary proteins (MUPs), each of which is thought to communicate different information. All three are encoded by large gene clusters in different regions of the mouse genome, clusters that have expanded dramatically during mouse evolutionary history. We report copy number variation among the most recently duplicated Abp genes, which suggests substantial volatility in this gene region. It appears that groups of these genes behave as low copy repeats (LCRs), duplicating as relatively large blocks of genes by nonallelic homologous …
Different Requirements Of The Swi/Snf Complex For Robust Nucleosome Displacement At Promoters Of Heat Shock Factor And Msn2- And Msn4-Regulated Heat Shock Genes, Tamara Y. Erkina, P. A. Tschetter, Alexander M. Erkine
Different Requirements Of The Swi/Snf Complex For Robust Nucleosome Displacement At Promoters Of Heat Shock Factor And Msn2- And Msn4-Regulated Heat Shock Genes, Tamara Y. Erkina, P. A. Tschetter, Alexander M. Erkine
Scholarship and Professional Work – COPHS
The stress response in yeast cells is regulated by at least two classes of transcription activators—HSF and Msn2/4, which differentially affect promoter chromatin remodeling. We demonstrate that the deletion of SNF2, an ATPase activity-containing subunit of the chromatin remodeling SWI/SNF complex, eliminates histone displacement, RNA polymerase II recruitment, and heat shock factor (HSF) binding at the HSP12 promoter while delaying these processes at the HSP82 and SSA4 promoters. Out of the three promoters, the double deletion of MSN2 and MSN4 eliminates both chromatin remodeling and HSF binding only at the HSP12 promoter, suggesting that Msn2/4 activators are primary determinants of …
Rapid Bursts Of Androgen-Binding Protein (Abp) Gene Duplication Occurred Independently In Diverse Mammals, Christina M. Laukaitis, Andreas Heger, Tyler D. Blakely, Pavel Munclinger, Chris P. Ponting, Robert C. Karn
Rapid Bursts Of Androgen-Binding Protein (Abp) Gene Duplication Occurred Independently In Diverse Mammals, Christina M. Laukaitis, Andreas Heger, Tyler D. Blakely, Pavel Munclinger, Chris P. Ponting, Robert C. Karn
Scholarship and Professional Work - LAS
Background
The draft mouse (Mus musculus) genome sequence revealed an unexpected proliferation of gene duplicates encoding a family of secretoglobin proteins including the androgen-binding protein (ABP) α, β and γ subunits. Further investigation of 14 α-like (Abpa) and 13 β- or γ-like (Abpbg) undisrupted gene sequences revealed a rich diversity of developmental stage-, sex- and tissue-specific expression. Despite these studies, our understanding of the evolution of this gene family remains incomplete. Questions arise from imperfections in the initial mouse genome assembly and a dearth of information about the gene family structure in other rodents …
Displacement Of Histones At Promoters Of Saccharomyces Cerevisiae Heat Shock Genes Is Differentially Associated With Histone H3 Acetylation, Tamara Y. Erkina, Alexander M. Erkine
Displacement Of Histones At Promoters Of Saccharomyces Cerevisiae Heat Shock Genes Is Differentially Associated With Histone H3 Acetylation, Tamara Y. Erkina, Alexander M. Erkine
Scholarship and Professional Work – COPHS
Chromatin remodeling at promoters of activated genes spans from mild histone modifications to outright displacement of nucleosomes in trans. Factors affecting these events are not always clear. Our results indicate that histone H3 acetylation associated with histone displacement differs drastically even between promoters of such closely related heat shock genes as HSP12, SSA4, and HSP82. The HSP12 promoter, with the highest level of histone displacement, showed the highest level of H3 acetylation, while the SSA4 promoter, with a lower histone displacement, showed only modest H3 acetylation. Moreover, for the HSP12 promoter, the level of acetylated H3 …
Diverse Spatial, Temporal, And Sexual Expression Of Recently Duplicated Androgen-Binding Protein Genes In Mus Musculus, Christina M. Laukaitis, Stephen R. Dlouhy, Richard D. Emes, Chris P. Ponting, Robert C. Karn
Diverse Spatial, Temporal, And Sexual Expression Of Recently Duplicated Androgen-Binding Protein Genes In Mus Musculus, Christina M. Laukaitis, Stephen R. Dlouhy, Richard D. Emes, Chris P. Ponting, Robert C. Karn
Scholarship and Professional Work - LAS
Background
The genes for salivary androgen-binding protein (ABP) subunits have been evolving rapidly in ancestors of the house mouse Mus musculus, as evidenced both by recent and extensive gene duplication and by high ratios of nonsynonymous to synonymous nucleotide substitution rates. This makes ABP an appropriate model system with which to investigate how recent adaptive evolution of paralogous genes results in functional innovation (neofunctionalization).
Results
It was our goal to find evidence for the expression of as many of the Abp paralogues in the mouse genome as possible. We observed expression of six Abpa paralogues and five Abpbg paralogues …
Cell Cycle-Dependent Binding Of Yeast Heat Shock Factor To Nucleosomes, Christina Bourgeois Venturi, Alexander M. Erkine, David S. Gross
Cell Cycle-Dependent Binding Of Yeast Heat Shock Factor To Nucleosomes, Christina Bourgeois Venturi, Alexander M. Erkine, David S. Gross
Scholarship and Professional Work – COPHS
In the nucleus, transcription factors must contend with the presence of chromatin in order to gain access to their cognate regulatory sequences. As most nuclear DNA is assembled into nucleosomes, activators must either invade a stable, preassembled nucleosome or preempt the formation of nucleosomes on newly replicated DNA, which is transiently free of histones. We have investigated the mechanism by which heat shock factor (HSF) binds to target nucleosomal heat shock elements (HSEs), using as our model a dinucleosomal heat shock promoter (hsp82-ΔHSE1). We find that activated HSF cannot bind a stable, sequence-positioned nucleosome in G1-arrested …
The Skn7 Response Regulator Of Saccharomyces Cerevisiae Interacts With Hsf1 In Vivo And Is Required For The Induction Of Heat Shock Genes By Oxidative Stress, Desmond C. Raitt, Anthony L. Johnson, Alexander M. Erkine, Kozo Makino, Brian Morgan, David S. Gross, Leland H. Johnston
The Skn7 Response Regulator Of Saccharomyces Cerevisiae Interacts With Hsf1 In Vivo And Is Required For The Induction Of Heat Shock Genes By Oxidative Stress, Desmond C. Raitt, Anthony L. Johnson, Alexander M. Erkine, Kozo Makino, Brian Morgan, David S. Gross, Leland H. Johnston
Scholarship and Professional Work – COPHS
The Skn7 response regulator has previously been shown to play a role in the induction of stress-responsive genes in yeast, e.g., in the induction of the thioredoxin gene in response to hydrogen peroxide. The yeast Heat Shock Factor, Hsf1, is central to the induction of another set of stress-inducible genes, namely the heat shock genes. These two regulatory trans-activators, Hsf1 and Skn7, share certain structural homologies, particularly in their DNA-binding domains and the presence of adjacent regions of coiled-coil structure, which are known to mediate protein–protein interactions. Here, we provide evidence that Hsf1 and Skn7 interact in vitro and …
Cooperative Binding Of Heat Shock Factor To The Yeast Hsp82 Promoter In Vivo And In Vitro, Alexander M. Erkine, Serena F. Magrogan, Edward A. Sekinger, David S. Gross
Cooperative Binding Of Heat Shock Factor To The Yeast Hsp82 Promoter In Vivo And In Vitro, Alexander M. Erkine, Serena F. Magrogan, Edward A. Sekinger, David S. Gross
Scholarship and Professional Work – COPHS
revious work has shown that heat shock factor (HSF) plays a central role in remodeling the chromatin structure of the yeastHSP82 promoter via constitutive interactions with its high-affinity binding site, heat shock element 1 (HSE1). The HSF-HSE1 interaction is also critical for stimulating both basal (noninduced) and induced transcription. By contrast, the function of the adjacent, inducibly occupied HSE2 and -3 is unknown. In this study, we examined the consequences of mutations in HSE1, HSE2, and HSE3 on HSF binding and transactivation. We provide evidence that in vivo, HSF binds to these three sites cooperatively. This cooperativity is seen …
Heat Shock Factor Gains Access To The Yeast Hsc82 Promoter Independently Of Other Sequence-Specific Factors And Antagonizes Nucleosomal Repression Of Basal And Induced Transcription, Alexander M. Erkine, C. C. Adams, T. Diken, D. S. Gross
Heat Shock Factor Gains Access To The Yeast Hsc82 Promoter Independently Of Other Sequence-Specific Factors And Antagonizes Nucleosomal Repression Of Basal And Induced Transcription, Alexander M. Erkine, C. C. Adams, T. Diken, D. S. Gross
Scholarship and Professional Work – COPHS
Transcription in eukaryotic cells occurs in the context of chromatin. Binding of sequence-specific regulatory factors must contend with the presence of nucleosomes for establishment of a committed preinitiation complex. Here we demonstrate that the high-affinity binding site for heat shock transcription factor (HSF) is occupied independently of other cis-regulatory elements and is critically required for preventing nucleosomal assembly over the yeast HSC82 core promoter under both noninducing (basal) and inducing conditions. Chromosomal mutation of this sequence, termed HSE1, erases the HSF footprint and abolishes both transcription and in vivo occupancy of the TATA box. Moreover, it dramatically reduces promoter chromatin …