Life Sciences Commons^™

Diseased Thyrocytes Have Aberrant Communication And Tnf Receptor Secretion, Mary Labue

Loma Linda University Electronic Theses, Dissertations & Projects

Autoimmune diseases are a world-wide health concern and are an intense area of investigation. However, one area of research has been largely ignored. This involves the contribution of the target tissue to the disease process. We hypothesized that cell-cell communication is disrupted by lymphocytic infiltration and advancing fibrosis and this disruption results in altered target cell responses to immune components produced at the site of inflammation, including Tumor Necrosis Factor-alpha (TNF- α).

We have used thyroiditis as our model of autoimmunity and have documented the development of spontaneous thyroiditis in MRL-lpr/lpr mice. We have found aberrant connexin expression in …

Circadian Clock-Controlled Genes Isolated From Neurospora Crassa Are Late Night- To Early Morning-Specific, Deborah Bell-Pedersen, Mari L. Shinohara, Jennifer J. Loros, Jay C. Dunlap

Dartmouth Scholarship

An endogenous circadian biological clock controls the temporal aspects of life in most organisms, including rhythmic control of genes involved in clock output pathways. In the fungus Neurospora crassa, one pathway known to be under control of the clock is asexual spore (conidia) development. To understand more fully the processes that are regulated by the N. crassa circadian clock, systematic screens were carried out for genes that oscillate at the transcriptional level. Time-of-day-specific cDNA libraries were generated and used in differential screens to identify six new clock-controlled genes (ccgs). Transcripts specific for each of the ccgs …

Cellular Basis Of Decreased Immune Responses To Pneumococcal Vaccines In Aged Mice, Manju Garg, Wei Luo, Alan M. Kaplan, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Previously, model systems were developed in our laboratory to study murine immune responses to the 23-valent pneumococcal polysaccharide vaccine Pnu-Imune, both in vivo and in vitro (M. Garg and B. Subbarao, Infect. Immun. 60:2329-2336, 1992; M. Garg, A. M. Kaplan, and S. Bondada, J. Immunol. 152: 1589-1596, 1994). Using these systems, we found that aged mice did not respond to the vaccine in vivo or in vitro. Cell separation studies showed that the unresponsiveness of the aged spleen cells to the vaccine was not due to an intrinsic B-cell defect or to T-cell-mediated immunosuppression but resulted from an accessory cell …

Shark Bay World Heritage Property: Summary Of Public Submissions To The Draft Management Plan For Fish Resources, Kevin A. Francesconi

Fisheries management papers

In November 1994, the draft management plan for fish resources for the Shark Bay World Heritage Area (Fisheries Management Paper no 72) was released for public comment. This report summarises and analyses the 28 written public submissions received to the draft fisheries management plan.

Genetic And Phylogenetic Divergence Of Feline Immunodeficiency Virus In The Puma (Puma Concolor), Margaret A. Carpenter, Eric W. Brown, Melanie Culver, Warren E. Johnson, Jill Pecon-Slattery, Dulce Brousset, Stephen J. O'Brien

Biology Faculty Articles

Feline immunodeficiency virus (FIV) is a lentivirus which causes an AIDS-like disease in domestic cats (Felis catus). A number of other felid species, including the puma (Puma concolor), carry a virus closely related to domestic cat FIV. Serological testing revealed the presence of antibodies to FIV in 22% of 434 samples from throughout the geographic range of the puma. FIV-Pco pol gene sequences isolated from pumas revealed extensive sequence diversity, greater than has been documented in the domestic cat. The puma sequences formed two highly divergent groups, analogous to the clades which have been defined for …

Shark Bay Management Paper For Fish Resources, Fisheries Department Of Western Australia, Department Of Conservation And Land Management

Fisheries management papers

This paper deals with the management of fish resources in the Shark Bay World Heritage Property which are the responsibility of the Fisheries Department of Western Australia. It was prepared by the Fisheries Department in close liaison with the Department of Conservation and Land Management. The policies and guidelines proposed in this paper will be followed for a period not exceeding 10 years.

Characterization Of Experimentally Induced, Nonaflatoxigenic Variant Strains Of Aspergillus Parasiticus., Shubha Kale Ireland, J. W. Cary, D. Bhatnagar, J. W. Bennett

Faculty and Staff Publications

Six previously isolated, nonaflatoxigenic variants of Aspergillus parasiticus, designated sec mutants, were characterized morphologically by electron microscopy, biochemically by biotransformation studies with an aflatoxin precursor, and genetically by Northern (RNA) hybridization analysis of aflatoxin biosynthetic gene transcripts. Scanning electron micrographs clearly demonstrated that compared with the parental sec+ forms, the variant sec forms had an abundance of vegetative mycelia, orders of magnitude reduced number of conidiophores and conidia, and abnormal metulae. Conidiospores were detected in sec cultures only at higher magnifications (x500), in contrast to the sec+ (wild-type) strain, in which abundant conidiospores (masking the vegetative mycelia) were observed even …

Prevalence Of Antibodies To Feline Parvovirus, Calicivirus, Herpesvirus, Coronavirus, And Immunodeficiency Virus And Of Feline Leukemia Virus Antigen And The Interrelationship Of These Viral Infections In Free-Ranging Lions In East Africa, Regina Hofmann-Lehmann, Daniela Fehr, Markus Grob, Muhamed Elgizoli, Craig Packer, Janice S. Martenson, Stephen J. O'Brien, Hans Lutz

Biology Faculty Articles

While viral infections and their impact are well studied in domestic cats, only limited information is available on their occurrence in free-ranging lions. The goals of the present study were (i) to investigate the prevalence of antibodies to feline calicivirus (FCV), herpesvirus (FHV), coronavirus (FCoV), parvovirus (FPV), and immunodeficiency virus (FIV) and of feline leukemia virus (FeLV) antigen in 311 serum samples collected between 1984 and 1991 from lions inhabiting Tanzania’s national parks and (ii) to evaluate the possible biological importance and the interrelationship of these viral infections. Antibodies to FCV, never reported previously in free-ranging lions, were detected in …

Pearling And Aquaculture In The Dampier Archipelago - Existing And Proposed Operations - A Report For Public Comment, Ben Fraser

Fisheries management papers

This discussion paper has been prepared by the Fisheries Department of WA as part of a consultative process to enable the public to comment on existing and proposed pearling and aquaculture activities in the Dampier Archipelago. This report follows on from Fisheries Management Report 90 - the Driscoll Report - and provides more detailed information about pearling and aquaculture to enable the public to consider and comment on individual projects.

Molecular Phylogeny And Typing Of Blackflies (Diptera: Simuliidae) That Serve As Vectors Of Human Or Bovine Onchocerciasis, Jianming Tang, Kenneth Pruess, Eddie W. Cupp, Thomas R. Unnasch

Department of Entomology: Faculty Publications

A subregion of the mitochondrial large subunit (16s) rRNA gene was amplified by polymerase chain reaction (PCR) from nine species of blackflies (Diptera: Simuliidae) which serve as natural or experimental vectors of human or bovine Onchocerca parasites. PCR products from each species of blackfly were tested by directed heteroduplex analysis (DHDA), and their genotypes established according to diagnostic banding patterns of the heteroduplex products. Three alleles of mitochondrial 16s rRNA were found to exist in members of the Simulium (Edwardsellum) damnosum sensu lato complex from West Africa, and two alleles were found in the Neotropical Simulium (Psilopelmia …

1: "To Know Ourselves", The U.S. Department Of Energy, The Human Genome Project

Genomics: Past & Future

AT THE END OF THE ROAD in Little Cottonwood Canyon, near Salt Lake City, Alta is a place of near-mythic renown among skiers. In time it may well assume similar status among molecular geneticists. In December 1984, a conference there, co-sponsored by the U.S. Department of Energy, pondered a single question: Does modern DNA research offer a way of detecting tiny genetic mutations—and, in particular, of observing any increase in the mutation rate among the survivors of the Hiroshima and Nagasaki bombings and their descendants? In short the answer was, Not yet. But in an atmosphere of rare intellectual fertility, …

Genome Research And Traditional Intellectual Property Protection -- A Bad Fit?, Kate H. Murashige

RISK: Health, Safety & Environment (1990-2002)

Dr. Murashige addresses the need for a patent system more closely tailored to the needs of biotechnology. For example, the obviousness requirement may interfere with using patents to recoup high costs of work when it could arguably be done by researchers of ordinary skill.

Offshore Constitutional Settlement 1995, H. G. Brayford, G. E. Lyon

Fisheries management papers

The Offshore Constitutional Settlement OCS is a legal arrangement between the Commonwealth and State Governments, and defines control over the fisheries which operate off each State of Australia. For the Western Australian coast, the first set of arrangements took effect in 1987. The OCS of 1995 specifies five arrangements, under which the fisheries inside the 200 nautical mile approximately 370 km limit of the Australian Fishing Zone AFZ come under either State, Commonwealth, or joint control.

Expression Of The Human Milk Protein, Beta-Casein : In Transgenic Potato Plants, Wendy Roberts

Loma Linda University Electronic Theses, Dissertations & Projects

Mother’s milk is recommended by the nutritional and pediatric communities as the best food for suckling infants. For non breast-fed infants, bovine milk-based formulas have been regarded as a suitable substitute. In the early 1900's, physicians found that foreign proteins in cow milk were responsible for gastrointestinal disease and even digestive collapse of the newborn fed a cow milk-based diet¹⁶. The allergenicity of cow milk for the human infant appears to be caused predominantly by p-casein¹⁷. In this research, the cDNA encoding human p-casein was introduced into potato cells under the control of the bidirectional, auxin-inducible …

Social Issues Of Genome Innovation And Intellectual Property, Elaine Alma Draper

RISK: Health, Safety & Environment (1990-2002)

Dr. Draper's focus is the use of personal information derived from genome research. She identifies several potential problems, including access to and control of genetic information, employment discrimination and social stratification. She also recommends possible solutions.

An Account Of Expert Phylogenetic Tree Construction From The Problem-Solving Research Tradition In Science Education, Steven D. Brewer

Dissertations

Content knowledge, strategic knowledge, and forms of knowledge organization used in phylogenetic tree construction are described to account for expert performance. A literature review is used to situate this research with respect to studies of: (a) the teaching of evolution, (b) problem-solving expertise, (c) physics and biology problem solving, and (d) the practice of phylogenetic systematics. Rationales are provided for the development of a computer-based problem-solving environment, model problems, and research problems and methodology.

Each problem consisted of a matrix of coded and polarized phylogenetic data organized by taxa and characters. Four series of research problems were constructed that varied …

Anion Induced Blue To Purple Transition In Bacteriorhodopsin, Mrunalini Pattarkine, Anil K. Singh

Faculty Works

Purple membrane (PM, λ" role="presentation">λmax" role="presentation">max 570 nm) of H. halobium on treatment with sulphuric acid changes its colour to blue (λ" role="presentation">λmax" role="presentation">max 608 nm). The purple chromophore can be regenerated from the blue chromophore by exogeneous addition of anions such as CI−" role="presentation">− and HPO42−" role="presentation">2−4. Chloride ion is found to be more effective than the dibasic phosphate ion in regenerating the purple chromophore. Nevertheless, one thing common to the anion regeneration is that both CI−" role="presentation">− and HPO42−" role="presentation">2−4 show marked pH effect. At pH 1.0 the efficiency of …

Metal-Resistance Genetically Engineered Bacteria, Sylvia Daunert, Donna Scott, Sridhar Ramanathan

KWRRI Research Reports

Bacterial-based electrochemical and optical sensing systems that respond in a highly selective and sensitive manner to antimonite and arsenite have been developed. This was accomplished by using genetically engineered bacteria bearing one of two plasmids constructed for our studies. The first plasmid, pBGD23, contains the operator/promoter region (O/P) and the gene of the ArsR protein from the ars operon upstream from the β-galactosidase gene. In the absence of antimonite/arsenite, ArsR binds to the 0/P site and prevents the transcription of the genes for ArsR and β-galactosidase, thus blocking expression of these proteins. When antimonite or arsenite is present in the …

Same Fish - Different Rules - Proceedings Of The National Fisheries Management Network Workshop Held As Part Of The Third Australasian Fisheries Managers Conference August 1995, F. B. Prokop

Fisheries management papers

This workshop represents the third time that a specific recreational fisheries workshop has been held in conjunction with the Australasian Fisheries Managers Conference. It is designed to analyse the different management approaches from the various fisheries agencies which have responsibility for selected species. The case studies that make up the workshop have been selected to represent the diversity of situations including freshwater, marine, pelagic and invertebrate species.

Fishermen's Views On The Future Management Of The Rock Lobster Fishery: A Report Prepared On Behalf Of The Rock Lobster Industry Advisory Committee, The Marketing Centre, Rock Lobster Industry Advisory Committee

Fisheries management papers

In June 1995, a self-completion survey of fishermen in the rock lobster fishery was sent out by The Marketing Centre to 589 licensees. A total of 206 valid responses were received. The questionnaire was fairly long and dealt with a number of complex issues. As such, this response rate of 36.0% compares favorably with the average for postal surveys of around 40.0%. The following represents the main findings from the survey.

A Report On The Issues Affecting The Use Of The Dampier Archipelago, Peter Driscoll

Fisheries management papers

This report was commissioned by the Minister for Fisheries following concerns about resource sharing, allocation of licences and leases for pearling and aquaculture, recreational and commercial fishing opportunities, and local residents continued ability to access the islands and marine areas of the Dampier Archipelago. The purpose of this report is to provide a scoping document for a marine planning study for the Dampier Archipelago for the Fisheries Department and the Minister for Fisheries.

Bad Ethics, Good Ethics And The Genetic Engineering Of Animals In Agriculture, Bernard Rollin

Genetics Collection

Genetic engineers have been remiss in addressing ethical and social issues emerging from this powerful new technology, a technology whose implications for agriculture are profound. As a consequence of this failure, society has been uneasy about genetic engineering of animals and has had difficulty distinguishing between genuine and spurious ethical issues the technology occasions. Many of the most prominent concerns do not require a serious response. On the other hand, concerns about a variety of possible risks arising from genetic engineering of animals require careful consideration and dialogue with the public. Such concerns are an admixture of ethics and prudence. …

Interactions Involving The Human Rna Polymerase Ii Transcription/Nucleotide Excision Repair Complex Tfiih, The Nucleotide Excision Repair Protein Xpg, And Cockayne Syndrome Group B (Csb) Protein, Narayan Iyer, Michael S. Reagan, Kou-Juey Wu, Bertram Canagarajah, Errol C. Friedberg

Biology Faculty Publications

The human basal transcription factor TFIIH plays a central role in two distinct processes. TFIIH is an obligatory component of the RNA polymerase II (RNAP II) transcription initiation complex. Additionally, it is believed to be the core structure around which some if not all the components of the nucleotide excision repair (NER) machinery assemble to constitute a nucleotide excision repairosome. At least two of the subunits of TFIIH (XPB and XPD proteins) are implicated in the disease xeroderma pigmentosum (XP). We have exploited the availability of the cloned XPB, XPD, p62, p44, and p34 genes (all …

Management Arrangements For Specimen Shell Collection In Western Australia, 1995, Jonathon Hs Barrington, C A. Campbell

Fisheries management papers

The Specimen Shell Fishery in Western Australia is based on the collection of marine species of the phylum Mollusca for the purposes of display, collection, cataloguing, classification and sale. Specimen shells may be alive or dead at the time of take and include beach-washed shells. Fossilised shells do not constitute specimen shells. This document has been prepared to detail the legislative requirements of the Western Australian Government, as well as to fulfill the requirements under the Commonwealth Wildlife Protection (Regulation of Exports and Imports) Act 1982 to allow for the export of specimen shells from Western Australian waters and the …

Balancing The Scales: Access And Equity In Fisheries Management - Proceedings Of The Third Australasian Fisheries Managers Conference, Rottnest Island, Western Australia, 2-4 August 1995, Patricia Summerfield

Fisheries management papers

The papers reproduced in this volume were presented at the Third Australasian Fisheries Managers Conference held at Kingstown Environmental Education Centre, Rottnest Island, Western Australia, from 2-4 August 1995. Two presentations have not been received in full written form and one paper was not available for publication due to copyright being held elsewhere. Abstracts of these papers are included in the proceedings. The papers are arranged in order of presentation at the Conference. The scenarios used in the workshop sessions are reproduced at the end of the proceedings for information.

A New Species Of Rock-Dwelling Phyllodactylus (Squamata: Gekkonidae) From The Richtersveld, South Africa, Aaron M. Bauer, William R. Branch, David A. Good

Occasional Papers of the Museum of Natural Science, Louisiana State University

No abstract provided.

A Miocene Hedgehog (Mammalia: Erinaceidae) From Fort Polk In Western Louisiana, Judith A. Schiebout

Occasional Papers of the Museum of Natural Science, Louisiana State University

No abstract provided.

Molecular Differentiation Of Alfalfa Weevil Strains (Coleoptera: Curculionidae), S. J. Erney, K. P. Pruess, S. D. Danielson, T. O. Powers

Department of Entomology: Faculty Publications

Mitochondrial DNA was amplified and sequenced from eastern, western, and Egyptian strains of alfalfa weevil, Hypera postica (Gyllenhal). Eastern and Egyptian weevils differed at only 2 nucleotide sites in 1,031 base pairs sequenced; western weevils differed by 5% sequence divergence. Three restriction sites were identified which separated eastern and western haplotypes. No intrastrain polymorphism was detected in 150 weevils from Nebraska. Collections from Lincoln in eastern Nebraska and Scottsbluff in western Nebraska were fixed for the eastern and western haplotypes, respectively. Eastern and western haplotypes were found together in the same fields in a broad overlap region in central Nebraska.

Heat Shock Factor Gains Access To The Yeast Hsc82 Promoter Independently Of Other Sequence-Specific Factors And Antagonizes Nucleosomal Repression Of Basal And Induced Transcription, Alexander M. Erkine, C. C. Adams, T. Diken, D. S. Gross

Scholarship and Professional Work – COPHS

Transcription in eukaryotic cells occurs in the context of chromatin. Binding of sequence-specific regulatory factors must contend with the presence of nucleosomes for establishment of a committed preinitiation complex. Here we demonstrate that the high-affinity binding site for heat shock transcription factor (HSF) is occupied independently of other cis-regulatory elements and is critically required for preventing nucleosomal assembly over the yeast HSC82 core promoter under both noninducing (basal) and inducing conditions. Chromosomal mutation of this sequence, termed HSE1, erases the HSF footprint and abolishes both transcription and in vivo occupancy of the TATA box. Moreover, it dramatically reduces promoter chromatin …

A Gene Correlate Of Geotaxis Near Adh (2-50.1) In Drosophila Melanogaster, Scott F. Stoltenberg, Jerry Hirsch

Department of Psychology: Faculty Publications

As part of a long-term study (38+ years) to identify genetic correlates of geotaxis in Drosophila melanogaster, the investigators report the results of allozyme-level analyses of 2 lines derived from hybridizing high- and low-selected lines that have evolved stable, extreme expressions of geotaxis. Allelic variation at Adh was associated with geotactic performance in 1 hybrid-derived line, but not in another, after 66 free-mating generations beyond the F₂ generation. A second-chromosome gene correlate of geotaxis may lie within 1 map unit of Adh. Population genetic analyses suggest that there were different selection pressures on the hybrid-derived lines and …