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Full-Text Articles in Life Sciences

Examining Phenotypic Structural Traits As Indicators For Reproductive Longevity Success In Sows, Lindsay Kate Peters, Melanie Dawn Trenhaile-Grannemann, Benny Edd Mote Apr 2020

Examining Phenotypic Structural Traits As Indicators For Reproductive Longevity Success In Sows, Lindsay Kate Peters, Melanie Dawn Trenhaile-Grannemann, Benny Edd Mote

UCARE Research Products

The objective of this study was to examine structure and body conformation traits of 494 gilts/sows as a prediction of reproductive longevity in sows. This study focused on specific traits including body length, body depth, front and rear pastern angles, knee angle, and hock angle. The primary method of data collection was based on videos. Still images were pulled from the videos when the sow was standing in a natural stance. The images were analyzed and interpreted objectively with Image J which gives the availability to measure distance and angles given known references in the images. The structural data was …


The Heritability Of Pampiniform Plexus Vessel Size And Varicocoele In Boars, Tasha R. Gruhot, Lea A. Rempel, Matthew L. Spangler, Stephen D. Kachman, Benny E. Mote Jan 2019

The Heritability Of Pampiniform Plexus Vessel Size And Varicocoele In Boars, Tasha R. Gruhot, Lea A. Rempel, Matthew L. Spangler, Stephen D. Kachman, Benny E. Mote

Department of Animal Science: Faculty Publications

Ultrasonography was used to capture a coronal–sagittal image of the veins of the pampiniform plexus (PP) and the testicular artery of 327 maternal‐line boars at approximately 6 months of age at the University of Nebraska—Lincoln. Varicocoele was diagnosed by two methods. Method 1 diagnosed varicocoele when the average vessel area on one side of the scrotum was 1.5 times larger than the average vessel area on the other side of the scrotum. Method 2 diagnosed varicocoele when the average vessel area on one side of the scrotum of a boar was 1.5 times larger than the average vessel on the …


Copy Number Variation In The Porcine Genome Detected From Whole-Genome Sequence, Rebecca Anderson Mar 2018

Copy Number Variation In The Porcine Genome Detected From Whole-Genome Sequence, Rebecca Anderson

Honors Theses

Copy number variations (CNVs) are large insertions, deletions, and duplications in the genome that vary between individuals in a species. These variations are known to impact a broad range of phenotypes from molecular-level traits to higher-order clinical phenotypes. CNVs have been linked to complex traits in humans such as autism, attention deficit hyperactivity disorder, nervous system disorders, and early-onset extreme obesity. In this study, whole-genome sequence was obtained from 72 founders of an intensely phenotyped experimental swine herd at the U.S. Meat Animal Research Center (USMARC) in Clay Center, Nebraska. This included 24 boars (12 Duroc and 12 Landrace) and …


The Impact Of Truncating Data On The Predictive Ability For Single-Step Genomic Best Linear Unbiased Prediction, Jeremy T. Howard, Thomas A. Rathje, Caitlyn E. Bruns, Danielle F. Wilson-Wells, Stephen D. Kachman, Matthew L. Spangler Jan 2018

The Impact Of Truncating Data On The Predictive Ability For Single-Step Genomic Best Linear Unbiased Prediction, Jeremy T. Howard, Thomas A. Rathje, Caitlyn E. Bruns, Danielle F. Wilson-Wells, Stephen D. Kachman, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Simulated and swine industry data sets were utilized to assess the impact of removing older data on the predictive ability of selection candidate estimated breeding values (EBV) when using single-step genomic best linear unbiased prediction (ssGBLUP). Simulated data included thirty replicates designed to mimic the structure of swine data sets. For the simulated data, varying amounts of data were truncated based on the number of ancestral generations back from the selection candidates. The swine data sets consisted of phenotypic and genotypic records for three traits across two breeds on animals born from 2003 to 2017. Phenotypes and genotypes were iteratively …


Fecal Microbiota Transplantation Is Associated With Reduced Morbidity And Mortality In Porcine Circovirus Associated Disease, Megan C. Niederwerder, Laura A. Constance, Raymond R. R. Rowland, Waseem Abbas, Samodha C. Fernando, Megan L. Potter, Maureen A. Sheahan, Thomas E. Burkey, Richard A. Hesse, Ada G. Cino-Ozuna Jan 2018

Fecal Microbiota Transplantation Is Associated With Reduced Morbidity And Mortality In Porcine Circovirus Associated Disease, Megan C. Niederwerder, Laura A. Constance, Raymond R. R. Rowland, Waseem Abbas, Samodha C. Fernando, Megan L. Potter, Maureen A. Sheahan, Thomas E. Burkey, Richard A. Hesse, Ada G. Cino-Ozuna

Department of Animal Science: Faculty Publications

Porcine circovirus associated disease (PCVAD) is a term used to describe the multifactorial disease syndromes caused by porcine circovirus type 2 (PCV-2), which can be reproduced in an experimental setting through the co-infection of pigs with PCV-2 and porcine reproductive and respiratory syndrome virus (PRRSV). The resulting PCVAD-affected pigs represent a subpopulation within the co-infected group. In co-infection studies, the presence of increased microbiome diversity is linked to a reduction in clinical signs. In this study, fecal microbiota transplantation (FMT) was investigated as a means to prevent PCVAD in pigs co-infected with PRRSV and PCV-2d. The sources of the FMT …


Genomics Symposium: Using Genomic Approaches To Uncover Sources Of Variation In Age At Puberty And Reproductive Longevity In Sows, Hiruni R. Wijesena, Clay A. Lents, Jean-Jack Riethoven, Melanie D. Trenhaile-Grannemann, Jennifer F. Thorson, Brittney N. Keel, Phillip S. Miller, Matt Spangler, Stephen D. Kachman, Daniel C. Ciobanu Jan 2017

Genomics Symposium: Using Genomic Approaches To Uncover Sources Of Variation In Age At Puberty And Reproductive Longevity In Sows, Hiruni R. Wijesena, Clay A. Lents, Jean-Jack Riethoven, Melanie D. Trenhaile-Grannemann, Jennifer F. Thorson, Brittney N. Keel, Phillip S. Miller, Matt Spangler, Stephen D. Kachman, Daniel C. Ciobanu

Department of Animal Science: Faculty Publications

Genetic variants associated with traits such as age at puberty and litter size could provide insight into the underlying genetic sources of variation impacting sow reproductive longevity and productivity. Genomewide characterization and gene expression profiling were used using gilts from the University of Nebraska–Lincoln swine resource population (n = 1,644) to identify genetic variants associated with age at puberty and litter size traits. From all reproductive traits studied, the largest fraction of phenotypic variation explained by the Porcine SNP60 BeadArray was for age at puberty (27.3%). In an evaluation data set, the predictive ability of all SNP from highranked 1-Mb …


Development Of An Objective Feet And Leg Conformation Evaluation Method Using Digital Imagery In Swine, J. D. Stock, J. A. Calderon Diaz, C. E. Abell, T. J. Baas, M. F. Rothschild, B. E. Mote, K. J. Stalder Jan 2017

Development Of An Objective Feet And Leg Conformation Evaluation Method Using Digital Imagery In Swine, J. D. Stock, J. A. Calderon Diaz, C. E. Abell, T. J. Baas, M. F. Rothschild, B. E. Mote, K. J. Stalder

Department of Animal Science: Faculty Publications

Background:The objectives of this study were to create an objective measurement method of joint angles for knee, hock, front and rear pasterns and a rear stance position in swine using digital imaging technology and to assess the repeatability of the objective measurement process. Methods and Findings: Forty-five multiparous sows (average parity 6.7 ± 2.5; parity range 5 to 14) from two commercial farms (n=21 farm 1 and n=24 farm 2) were used. Sows were moved to a pen where digital images of the profile and rear stance were captured. On average, 5.2 (± 2.6) profile and 2.6 (± 1.0) …


Evaluation Of Reduced Subsets Of Single Nucleotide Polymorphisms For The Prediction Of Age At Puberty In Sows, Katherine L. Lucot, Matthew L. Spangler, Melanie D. Trenhaile, Stephen D. Kachman, Daniel C. Ciobanu Aug 2015

Evaluation Of Reduced Subsets Of Single Nucleotide Polymorphisms For The Prediction Of Age At Puberty In Sows, Katherine L. Lucot, Matthew L. Spangler, Melanie D. Trenhaile, Stephen D. Kachman, Daniel C. Ciobanu

Department of Animal Science: Faculty Publications

Genomic information could be used efficiently to improve traits that are expensive to measure, sex limited or expressed late in life. This study analyzed the phenotypic variation explained by major SNPs and windows for age at puberty in gilts, an indicator of reproductive longevity. A genome-wide association study using 56,424 SNPs explained 25.2% of the phenotypic variation in age at puberty in a training set (n = 820). All SNPs from the top 10% of 1-Mb windows explained 33.5% of the phenotypic variance compared to 47.1% explained by the most informative markers (n = 261). In an evaluation population, consisting …


Genome-Wide Analysis Of Tnf-Alpha Response In Pigs Challenged With Porcine Circovirus 2b, C. A. Kreikemeier, T. B., K. L. Lucot, Thomas E. Burkey, Daniel C. Ciobanu Apr 2015

Genome-Wide Analysis Of Tnf-Alpha Response In Pigs Challenged With Porcine Circovirus 2b, C. A. Kreikemeier, T. B., K. L. Lucot, Thomas E. Burkey, Daniel C. Ciobanu

Department of Animal Science: Faculty Publications

Tumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine with a role in activating adaptive immunity to viral infections. By inhibiting the capacity of plasmacytoid dendritic cells to produce interferon-α and TNF-α, porcine circovirus 2 (PCV2) limits the maturation of myeloid dendritic cells and impairs their ability to recognize viral and bacterial antigens. Previously, we reported QTL for viremia and immune response in PCV2- infected pigs. In this study, we analyzed phenotypic and genetic relationships between TNFα protein levels, a potential indicator of predisposition to PCV2 co-infection, and PCV2 susceptibility. Following experimental challenge with …


Analysis Of Long-Term Selection (28 Generations) For Reproduction, Growth, And Carcass Traits In Swine, Wan-Ling Hsu May 2011

Analysis Of Long-Term Selection (28 Generations) For Reproduction, Growth, And Carcass Traits In Swine, Wan-Ling Hsu

Department of Animal Science: Dissertations, Theses, and Student Research

The objectives were 1) to estimate responses from 28 generations of selection for increased pigs born alive (BA) with additional selection for increased 180 d weight (WT) and longissimus muscle area (LMA) and decreased backfat (BF) in the last eight generations, 2) to estimate genetic parameters for an array of traits, and 3) to investigate whether a plateau in response for BA has occurred. All lines were derived from the same Large White/Landrace composite population. Index selection for ovulation rate and embryo survival (G0-11) was initiated in L2 in 1981; L1 was selected randomly control line for L2 (G0-23). L2 …


Effect Of Hydrogen Peroxide In The Scald Tank On The Microbial Count Of Pork Skin, A. S. De Mello Jr., R. O. Roca Jan 2009

Effect Of Hydrogen Peroxide In The Scald Tank On The Microbial Count Of Pork Skin, A. S. De Mello Jr., R. O. Roca

Department of Animal Science: Faculty Publications

The objective of this study was to investigate the effect of hydrogen peroxide (H2O2) (50% v/v) on pork skin microbial populations. Forty-eight crossbred hogs were analyzed after dehairing and 30 during chilling. Three different concentrations of hydrogen peroxide were added to the scalding water (0.01, 0.05 and 0.1% of the total capacity of the scald tank). In treatment I no addition of H2O2 was applied while, in treatment II, H2O2 was added at 0 min and, in treatment III, it was added at 0, 30 and 60 min. Both treatments …