Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Beef cattle (6)
- Feedlot (3)
- Cytokine (2)
- Dried distillers grains (2)
- Evolution (2)
-
- Gene loss (2)
- Grain adaptation (2)
- Pathogenesis (2)
- Polyploidy (2)
- Single-nucleotide polymorphism (2)
- Speciation (2)
- Supplementation (2)
- 5‐HTTLPR (1)
- AFLP (1)
- Amplified fragment length polymorphism (1)
- Anaerobic digester (1)
- Animal unit (1)
- Animal waste (1)
- Arabidopsis MTM1 (1)
- Archaeological maize (1)
- Beef cattle; heterosis; growth traits (1)
- Beef production system (1)
- Beef; calving difficulty; breed effects (1)
- Biodiversity conservation (1)
- Bioinformatics (1)
- Body composition (1)
- Calving date (1)
- Chaco Canyon (1)
- Comparative genomics (1)
- Copper (1)
- Publication
-
- Department of Animal Science: Faculty Publications (20)
- Nebraska Center for Virology: Faculty Publications (12)
- Department of Entomology: Faculty Publications (5)
- Department of Agronomy and Horticulture: Faculty Publications (4)
- Jay F. Storz Publications (2)
-
- University of Nebraska State Museum: Mammalogy Papers (2)
- Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research (1)
- Department of Electrical and Computer Engineering: Dissertations, Theses, and Student Research (1)
- Department of Psychology: Faculty Publications (1)
- Eileen Hebets Publications (1)
- INTSORMIL Scientific Publications (1)
- Lawrence G. Harshman Publications (1)
- School of Biological Sciences: Faculty Publications (1)
- United States Geological Survey: Staff Publications (1)
Articles 1 - 30 of 53
Full-Text Articles in Life Sciences
Intsormil- Centa’S New Bmr Sorghum Varieties Create A Surge In Dairy Production, René Clará Valencia
Intsormil- Centa’S New Bmr Sorghum Varieties Create A Surge In Dairy Production, René Clará Valencia
INTSORMIL Scientific Publications
Scientists from the INTSORMIL-CENTA project based at the National Center for Agricultural Technology and Forestry (CENTA) of El Salvador have worked for five years to develop varieties of sorghum for grain and forage that are highly nutritious to cattle. Using the gene bmr-12, received from Dr. Gebisa Ejeta of Purdue University, they combined commercial varieties CENTA S-2, S-3 CENTA, CENTA RCV and VG 146 and were able to form new varieties containing this same gene. The first generation created 76 new varieties.
Genetic Diversity Of Neotropical Myotis (Chiroptera: Vespertilionidae) With An Emphasis On South American Species, Roxanne J. Larsen, Michelle C. Knapp, Hugh H. Genoways, Faisal Ali Anwarali Khan, Peter A. Larsen, Don E. Wilson, Robert J. Baker
Genetic Diversity Of Neotropical Myotis (Chiroptera: Vespertilionidae) With An Emphasis On South American Species, Roxanne J. Larsen, Michelle C. Knapp, Hugh H. Genoways, Faisal Ali Anwarali Khan, Peter A. Larsen, Don E. Wilson, Robert J. Baker
University of Nebraska State Museum: Mammalogy Papers
Background: Cryptic morphological variation in the Chiropteran genus Myotis limits the understanding of species boundaries and species richness within the genus. Several authors have suggested that it is likely there are unrecognized species-level lineages of Myotis in the Neotropics. This study provides an assessment of the diversity in New World Myotis by analyzing cytochrome-b gene variation from an expansive sample ranging throughout North, Central, and South America. We provide baseline genetic data for researchers investigating phylogeographic and phylogenetic patterns of Myotis in these regions, with an emphasis on South America.
Methodology and Principal Findings: Cytochrome-b sequences were generated and …
Serotonin System Gene Polymorphisms Are Associated With Impulsivity In A Context Dependent Manner, Scott F. Stoltenberg, Christa C. Christ, Krista B. Highland
Serotonin System Gene Polymorphisms Are Associated With Impulsivity In A Context Dependent Manner, Scott F. Stoltenberg, Christa C. Christ, Krista B. Highland
Department of Psychology: Faculty Publications
Impulsivity is a risk factor for adverse outcomes and characterizes several psychiatric disorders and risk for suicide. There is strong evidence that genetic variation influences individual differences in impulsivity, but the details are not yet understood. There is growing interest in better understanding the context dependency of genetic effects that is reflected in studies examining gender specificity, gene × environment interaction and epistasis (gene-gene interaction). In a cross-sectional study we examined whether polymorphisms in six serotonin system candidate genes and the experience of early life trauma (age 0–12) were associated with individual differences in impulsivity in a nonclinical sample of …
Organellar Signaling Expands Plant Phenotypic Variation And Increases The Potential For Breeding The Epigenome, Roberto De La Rosa Santamaria
Organellar Signaling Expands Plant Phenotypic Variation And Increases The Potential For Breeding The Epigenome, Roberto De La Rosa Santamaria
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
MUTS HOMOLOGUE 1 (MSH1) is a nuclear gene unique to plants that functions in mitochondria and plastids, where it confers genome stability. Phenotypic effects of MSH1 down- regulation were studied in sorghum inbreed line Tx430 and Arabidopsis ecotype Columbia-0, with the hypothesis that RNAi suppression of MSH1 triggers retrograde signaling from organelles to the nucleus, alters the epigenome, and derives heritable phenotypic variation suitable for artificial selection. An array of morphological traits and metabolic pathways was detected, including leaf variegation, male sterility and dwarfism, associated with altered gibberellic acid metabolism, higher levels of reactive oxygen species (ROS), and decreased synthesis …
Rosette Iron Deficiency Transcript And Microrna Profiling Reveals Links Between Copper And Iron Homeostasis In Arabidopsis Thaliana, Brian M. Waters, Samuel A. Mcinturf, Ricardo J. Stein
Rosette Iron Deficiency Transcript And Microrna Profiling Reveals Links Between Copper And Iron Homeostasis In Arabidopsis Thaliana, Brian M. Waters, Samuel A. Mcinturf, Ricardo J. Stein
Department of Agronomy and Horticulture: Faculty Publications
Iron (Fe) is an essential plant micronutrient, and its deficiency limits plant growth and development on alkaline soils. Under Fe deficiency, plant responses include up-regulation of genes involved in Fe uptake from the soil. However, little is known about shoot responses to Fe deficiency. Using microarrays to probe gene expression in Kas-1 and Tsu-1 ecotypes of Arabidopsis thaliana, and comparison with existing Col-0 data, revealed conserved rosette gene expression responses to Fe deficiency. Fe-regulated genes included known metal homeostasis-related genes, and a number of genes of unknown function. Several genes responded to Fe deficiency in both roots and rosettes. …
Mutations In Dmrt3 Affect Locomotion In Horses And Spinal Circuit Function In Mice, Lisa S. Andersson, Martin Larhammar, Fatima Memic, Hanna Wootz, Doreen Schwochow, Carl-Johan Rubin, Kalicharan Patra, Thorvaldur Arnason, Lisbeth Wellbring, Göran Hjälm, Freyja Imsland, Jessica Lynn Petersen, Molly E. Mccue, James R. Mickelson, Gus Cothran, Nadav Ahituv, Lars Roepstorff, Sofia Mikko, Anna Vallstedt, Gabriella Lindgren, Leif Andersson, Klas Kullander
Mutations In Dmrt3 Affect Locomotion In Horses And Spinal Circuit Function In Mice, Lisa S. Andersson, Martin Larhammar, Fatima Memic, Hanna Wootz, Doreen Schwochow, Carl-Johan Rubin, Kalicharan Patra, Thorvaldur Arnason, Lisbeth Wellbring, Göran Hjälm, Freyja Imsland, Jessica Lynn Petersen, Molly E. Mccue, James R. Mickelson, Gus Cothran, Nadav Ahituv, Lars Roepstorff, Sofia Mikko, Anna Vallstedt, Gabriella Lindgren, Leif Andersson, Klas Kullander
Department of Animal Science: Faculty Publications
Locomotion in mammals relies on a central pattern-generating circuitry of spinal interneurons established during development that coordinates limb movement. These networks produce left–right alternation of limbs as well as coordinated activation of flexor and extensor muscles. Here we show that a premature stop codon in the DMRT3 gene has a major effect on the pattern of locomotion in horses. The mutation is permissive for the ability to perform alternate gaits and has a favorable effect on harness racing performance. Examination of wild-type and Dmrt3-null mice demonstrates that Dmrt3 is expressed in the dI6 subdivision of spinal cord neurons, takes …
Classification Of Genomic Sequences By Latent Semantic Analysis, Samuel F. Way
Classification Of Genomic Sequences By Latent Semantic Analysis, Samuel F. Way
Department of Electrical and Computer Engineering: Dissertations, Theses, and Student Research
Evolutionary distance measures provide a means of identifying and organizing related organisms by comparing their genomic sequences. As such, techniques that quantify the level of similarity between DNA sequences are essential in our efforts to decipher the genetic code in which they are written.
Traditional methods for estimating the evolutionary distance separating two genomic sequences often require that the sequences first be aligned before they are compared. Unfortunately, this preliminary step imposes great computational burden, making this class of techniques impractical for applications involving a large number of sequences. Instead, we desire new methods for differentiating genomic sequences that eliminate …
Developmental Programming In Response To Intrauterine Growth Restriction Impairs Myoblast Function And Skeletal Muscle Metabolism, Dustin T. Yates, A.R. Macko, M. Nearing, X. Chen, R.P. Rhoads, Sean W. Limesand
Developmental Programming In Response To Intrauterine Growth Restriction Impairs Myoblast Function And Skeletal Muscle Metabolism, Dustin T. Yates, A.R. Macko, M. Nearing, X. Chen, R.P. Rhoads, Sean W. Limesand
Department of Animal Science: Faculty Publications
Fetal adaptations to placental insufficiency alter postnatal metabolic homeostasis in skeletal muscle by reducing glucose oxidation rates, impairing insulin action, and lowering the proportion of oxidative fibers. In animal models of intrauterine growth restriction (IUGR), skeletalmuscle fibers have lessmyonuclei at birth. This means thatmyoblasts, the sole source formyonuclei accumulation in fibers, are compromised. Fetal hypoglycemia and hypoxemia are complications that result from placental insufficiency. Hypoxemia elevates circulating catecholamines, and chronic hypercatecholaminemia has been shown to reduce fetal muscle development and growth. We have found evidence for adaptations in adrenergic receptor expression profiles in myoblasts and skeletal muscle of IUGR sheep …
Escape From Preferential Retention Following Repeated Whole Genome Duplications In Plants, James C. Schnable, Xiaowu Wang, J. Chris Pires, Michael Freeling
Escape From Preferential Retention Following Repeated Whole Genome Duplications In Plants, James C. Schnable, Xiaowu Wang, J. Chris Pires, Michael Freeling
Department of Agronomy and Horticulture: Faculty Publications
The well supported gene dosage hypothesis predicts that genes encoding proteins engaged in dose–sensitive interactions cannot be reduced back to single copies once all interacting partners are simultaneously duplicated in a whole genome duplication. The genomes of extant flowering plants are the result of many sequential rounds of whole genome duplication, yet the fraction of genomes devoted to encoding complex molecular machines does not increase as fast as expected through multiple rounds of whole genome duplications. Using parallel interspecies genomic comparisons in the grasses and crucifers, we demonstrate that genes retained as duplicates following a whole genome duplication have only …
Genetic Variation In Field And Laboratory Populations Of The Spined Soldier Bug, Podisus Maculiventris, Kathleen Kneeland, Thomas A. Coudron, Erica Lindroth, David Stanley, John E. Foster
Genetic Variation In Field And Laboratory Populations Of The Spined Soldier Bug, Podisus Maculiventris, Kathleen Kneeland, Thomas A. Coudron, Erica Lindroth, David Stanley, John E. Foster
Department of Entomology: Faculty Publications
The predatory spined soldier bug, Podisus maculiventris (Say) (Heteroptera: Pentatomidae), is an economically important and highly valued biological control agent. There is substantial information on the biology, ecology, behavior, and rearing of this stink bug. However, virtually nothing is known of its genetic variation, in natural or domesticated populations. To address this lacuna, we used amplified fragment length polymorphism (AFLP) to assess the genetic variability of field and laboratory populations. Four AFLP universal primer combinations yielded a total of 209 usable loci. The AFLP results showed greater genetic variability between populations from Missouri and Mississippi (both USA), and relatively low …
Characterization Of Species C Human Adenovirus Serotype 6 (Ad6), Eric A. Weaver, Reeti Khare, Mathew L. Hillestad, Donna Palmer, Philip Ng, Michael A. Barry
Characterization Of Species C Human Adenovirus Serotype 6 (Ad6), Eric A. Weaver, Reeti Khare, Mathew L. Hillestad, Donna Palmer, Philip Ng, Michael A. Barry
Nebraska Center for Virology: Faculty Publications
Adenovirus serotype (Ad5) is the most studied Ad. Ad1, 2, and 6 are also members of species C Ad and are presumed to have biologies similar to Ad5. In this work, we have compared the ability of Ad1, 2, 5, and 6 to infect liver and muscle after intravenous and intramuscular injection. We found that Ad6 was surprisingly the most potent at liver gene delivery and that Ad1 and Ad2 were markedly weaker than Ad5 and 6. To understand these differences, we sequenced the Ad6 genome. This revealed that the Ad6 fiber protein is surprisingly three shaft repeats shorter than …
Heme Oxygenase-1 Regulates The Immune Response To Influenza Virus Infection And Vaccination In Aged Mice, Nathan W. Cummins, Eric A. Weaver, Shannon M. May, Anthony J. Croatt, Oded Foreman, Richard B. Kennedy, Gregory A. Poland, Michael A. Barry, Karl A. Nath, Andrew D. Badley
Heme Oxygenase-1 Regulates The Immune Response To Influenza Virus Infection And Vaccination In Aged Mice, Nathan W. Cummins, Eric A. Weaver, Shannon M. May, Anthony J. Croatt, Oded Foreman, Richard B. Kennedy, Gregory A. Poland, Michael A. Barry, Karl A. Nath, Andrew D. Badley
Nebraska Center for Virology: Faculty Publications
Underlying mechanisms of individual variation in severity of influenza infection and response to vaccination are poorly understood. We investigated the effect of reduced heme oxygenase-1 (HO-1) expression on vaccine response and outcome of influenza infection. HO-1-deficient and wild-type (WT) mice (kingdom, Animalia; phylum, Chordata; genus/species, Mus musculus) were infected with influenza virus A/PR/8/34 with or without prior vaccination with an adenoviral-based influenza vaccine. A genome-wide association study evaluated the expression of single-nucleotide polymorphisms (SNPs) in the HO-1 gene and the response to influenza vaccination in healthy humans. HO-1-deficient mice had decreased survival after influenza infection compared to WT mice (median …
Use Of A Complete-Feed Diet (Ramp) To Rapidly Transition Cattle To A Finishing Diet, C. J. Schneider, Brandon L. Nuttelman, Dirk Burken, Terry J. Klopfenstein, Galen E. Erickson
Use Of A Complete-Feed Diet (Ramp) To Rapidly Transition Cattle To A Finishing Diet, C. J. Schneider, Brandon L. Nuttelman, Dirk Burken, Terry J. Klopfenstein, Galen E. Erickson
Department of Animal Science: Faculty Publications
A study evaluated transitioning cattle from RAMP to a finishing diet with fewer intermediate step diets and days fed during transition. Yearling crossbred steers (n = 390; BW = 341 ± 14 kg) were blocked and stratified by BW, and assigned randomly within strata to 40 feedlot pens, with 9 or 10 steers per pen. Treatments were different grain adaptation programs (27 or 28 d) involving blends of RAMP and finishing diet. A control adaptation program involved decreasing RAMP and increasing a 62.5% corn finishing diet (62.5% high moisture corn (HMC), 25% sweet bran, 7.5% alfalfa hay (AH), and 5% …
Use Of A Complete-Feed Diet (Ramp) In Grain Adaptation Programs Compared To Traditional Grain Adaptation Programs, C. J. Schneider, Brandon L. Nuttelman, Kelsey M. Rolfe Rolfe, William A. Griffin, Terry J. Klopfenstein, Galen E. Erickson
Use Of A Complete-Feed Diet (Ramp) In Grain Adaptation Programs Compared To Traditional Grain Adaptation Programs, C. J. Schneider, Brandon L. Nuttelman, Kelsey M. Rolfe Rolfe, William A. Griffin, Terry J. Klopfenstein, Galen E. Erickson
Department of Animal Science: Faculty Publications
A study evaluated using RAMP to adapt cattle to a high grain finishing diet. Yearling crossbred steers (n = 229; BW = 397 ± 28.4 kg) were consecutively weighed over 2 d, stratified by BW, and assigned randomly within strata to 18 feedlot pens, with 12 or 13 steers per pen. Treatments were imposed during grain adaptation (22 d) by decreasing RAMP (100 to 0%) and increasing finishing diet (0 to 100%). RAMP was fed either as a blend in a 1-diet system which was delivered twice daily (RAMP-1RS) or as 2 daily diets where RAMP was delivered in one …
Occurrence Of Hormonal Residues In Feedlot Cattle Waste, Terry L. Mader, Leslie J. Johnson, Shannon L. Bartelt-Hunt, Daniel D. Snow, William Kranz, Charles Shapiro, Simon Van Donk, David P. Shelton, David Tarkalson, T. C. Zhang, Steve Ensley
Occurrence Of Hormonal Residues In Feedlot Cattle Waste, Terry L. Mader, Leslie J. Johnson, Shannon L. Bartelt-Hunt, Daniel D. Snow, William Kranz, Charles Shapiro, Simon Van Donk, David P. Shelton, David Tarkalson, T. C. Zhang, Steve Ensley
Department of Animal Science: Faculty Publications
Two identically designed studies were conducted in separate years at the UNL Haskell Agricultural Laboratory using 192 crossbred heifers (96/study). Within a study heifers (initial weight = 386kg) were randomly assigned to 2 groups (3 42.4 m × 14.6 m pens/group/year): 1) treatment (TRT) animals were administered synthetic hormones via subcutaneous implants [d 1, 36 mg zearalonal; d 35, 140 mg trenbolone acetate (TBA) and 14 mg of 17β-estradiol benzoate (Revalor-H)] and fed Melengesterol Acetate (MGA), and 2) control (CON) animals with no synthetic hormone provided. Throughout each study, feedlot pen surface samples were obtained from 4 equally-sized zones (5 …
Spatial Genetic Variation Among Spodoptera Frugiperda (Lepidoptera: Noctuidae) Sampled From The United States, Puerto Rico, Panama, And Argentina, Difabachew K. Belay, Pete L. Clark, Steven R. Skoda, David J. Isenhour, Jaime Molina-Ochoa, Claudia Gianni, John E. Foster
Spatial Genetic Variation Among Spodoptera Frugiperda (Lepidoptera: Noctuidae) Sampled From The United States, Puerto Rico, Panama, And Argentina, Difabachew K. Belay, Pete L. Clark, Steven R. Skoda, David J. Isenhour, Jaime Molina-Ochoa, Claudia Gianni, John E. Foster
Department of Entomology: Faculty Publications
Spatial genetic variability of the fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), was studied by collecting samples from 31 locations in the United States, Argentina, Panama, and Puerto Rico, and then using amplified fragment length polymorphism to detect genetic variation. Analysis of molecular variance showed significant genetic variation in fall armyworm among all (28%) sample locations and individuals within (71%) sample locations; genetic variation of fall armyworm was minimal between sample locations grouped into regions. The pairwise fixation index (FST) comparisons showed significant genetic differentiation (0.288) among the 31 locations. However, dendrograms of results from cluster …
Anaerobic Digestion Of Finishing Cattle Manure With And Without Distillers Grains In The Diet., Andrea K. Watson, Terry J. Klopfenstein, Galen E. Erickson, Samodha C. Fernando, Jana L. Harding, Adam L. Shreck
Anaerobic Digestion Of Finishing Cattle Manure With And Without Distillers Grains In The Diet., Andrea K. Watson, Terry J. Klopfenstein, Galen E. Erickson, Samodha C. Fernando, Jana L. Harding, Adam L. Shreck
Department of Animal Science: Faculty Publications
Two treatments (cattle diet) were used in anaerobic digestion of finishing cattle manure. Diet 1 contained 82.5% dry rolled corn (DRC) (CONT) and diet 2 contained 40% wet distillers grains plus solubles (DM basis) replacing DRC (WDGS). Cattle were housed in stanchions for 3 d with complete manure collection in a cement pit. Manure (feces and urine) was sampled and analyzed for minerals, DM and OM. Manure collected was weighed into individual allotments based on DM and frozen. Continuously stirred anaerobic digesters (n = 7, 1 L capacity) were inoculated and maintained for 5 mo before the trial to ensure …
Vegfa Family Isoforms Regulate Spermatogonial Stem Cell Homeostasis In Vivo, Kyle C. Caires, Jeanene M. Clopton, Andrea S. Cupp, Derek J. Mclean
Vegfa Family Isoforms Regulate Spermatogonial Stem Cell Homeostasis In Vivo, Kyle C. Caires, Jeanene M. Clopton, Andrea S. Cupp, Derek J. Mclean
Department of Animal Science: Faculty Publications
The objective of the present study was to investigate vascular endothelial growth factor A (VEGFA) isoform regulation of cell fate decisions of spermatogonial stem cells (SSC) in vivo. The expression pattern and cell-specific distribution of VEGF isoforms, receptors, and coreceptors during testis development postnatal d 1–180 suggest a nonvascular function for VEGF regulation of early germ cell homeostasis. Populations of undifferentiated spermatogonia present shortly after birth were positive for VEGF receptor activation as demonstrated by immunohistochemical analysis. Thus, we hypothesized that proangiogenic isoforms of VEGF (VEGFA164) stimulate SSC self-renewal, whereas antiangiogenic isoforms of VEGF (VEGFA165b) …
Genome-Wide Analysis Of Syntenic Gene Deletion In The Grasses, James C. Schnable, Michael Freeling, Eric Lyons
Genome-Wide Analysis Of Syntenic Gene Deletion In The Grasses, James C. Schnable, Michael Freeling, Eric Lyons
Department of Agronomy and Horticulture: Faculty Publications
The grasses, Poaceae, are one of the largest and most successful angiosperm families. Like many radiations of flowering plants, the divergence of the major grass lineages was preceded by a whole-genome duplication (WGD), although these events are not rare for flowering plants. By combining identification of syntenic gene blocks with measures of gene pair divergence and different frequencies of ancient gene loss, we have separated the two subgenomes present in modern grasses. Reciprocal loss of duplicated genes or genomic regions has been hypothesized to reproductively isolate populations and, thus, speciation. However, in contrast to previous studies in yeast and teleost …
Divergent Functions Of The Myotubularin (Mtm) Homologs Atmtm1 And Atmtm2 In Arabidopsis Thaliana: Evolution Of The Plant Mtm Family, Yong Ding, Ivan Ndamukong, Yang Zhao, Yuannan Xia, Jean-Jack Riethoven, David R. Jones, Nullin Divecha, Zoya Avramova
Divergent Functions Of The Myotubularin (Mtm) Homologs Atmtm1 And Atmtm2 In Arabidopsis Thaliana: Evolution Of The Plant Mtm Family, Yong Ding, Ivan Ndamukong, Yang Zhao, Yuannan Xia, Jean-Jack Riethoven, David R. Jones, Nullin Divecha, Zoya Avramova
School of Biological Sciences: Faculty Publications
Myotubularin and myotubularin-related proteins are evolutionarily conserved in eukaryotes. Defects in their function result in muscular dystrophy, neuronal diseases, and leukemia in humans. In contrast to the animal lineage, where genes encoding both active and inactive myotubularins (phosphoinositide 3-phosphatases) have appeared and proliferated in the basal metazoan group, myotubularin genes are not found in the unicellular relatives of green plants. However, they are present in land plants encoding proteins highly similar to the active metazoan enzymes. Despite their remarkable structural conservation, plant and animal myotubularins have significantly diverged in their functions. While loss of myotubularin function causes severe disease phenotypes …
Distribution Of Genes And Repetitive Elements In The Diabrotica Virgifera Virgifera Genome Estimated Using Bac Sequencing, Brad S. Coates, Analiza P. Alves, Haichuan Wang, Kimberly K. O. Walden, B. Wade French, Nicholas J. Miller, Craig A. Abel, Hugh M. Robertson, Thomas W. Sappington, Blair D. Siegfried
Distribution Of Genes And Repetitive Elements In The Diabrotica Virgifera Virgifera Genome Estimated Using Bac Sequencing, Brad S. Coates, Analiza P. Alves, Haichuan Wang, Kimberly K. O. Walden, B. Wade French, Nicholas J. Miller, Craig A. Abel, Hugh M. Robertson, Thomas W. Sappington, Blair D. Siegfried
Department of Entomology: Faculty Publications
Feeding damage caused by the western corn rootworm, Diabrotica virgifera virgifera, is destructive to corn plants in North America and Europe where control remains challenging due to evolution of resistance to chemical and transgenic toxins. A BAC library, DvvBAC1, containing 109,486 clones with 104 ± 34.5 kb inserts was created, which has an ~4.56X genome coverage based upon a 2.58 Gb (2.80 pg) flow cytometry-estimated haploid genome size. Paired end sequencing of 1037 BAC inserts produced 1.17Mb of data (~0.05% genome coverage) and indicated ~9.4 and 16.0% of reads encode, respectively, endogenous genes and transposable elements (TEs). …
Inbreeding-Stress Interactions: Evolutionary And Conservation Consequences, David H. Reed, Charles W. Fox, Laramy S. Enders, Torsten N. Kristensen
Inbreeding-Stress Interactions: Evolutionary And Conservation Consequences, David H. Reed, Charles W. Fox, Laramy S. Enders, Torsten N. Kristensen
Department of Entomology: Faculty Publications
The effect of environmental stress on the magnitude of inbreeding depression has a long history of intensive study. Inbreeding-stress interactions are of great importance to the viability of populations of conservation concern and have numerous evolutionary ramifications. However, such interactions are controversial. Several meta-analyses over the last decade, combined with omic studies, have provided considerable insight into the generality of inbreeding-stress interactions, its physiological basis, and have provided the foundation for future studies. In this review, we examine the genetic and physiological mechanisms proposed to explain why inbreeding-stress interactions occur. We specifically examine whether the increase in inbreeding depression with …
Effects Of Feeding Field Peas In Combination With Distillers Grains Plus Solubles In Finishing And Growing Diets On Cattle Performance And Carcass Characteristics, A. C. Pesta, A. H. Titlow, J. A. Hansen, A. L. Berger, S. A. Furman, Matt K. Luebbe, G. E. Erickson, K. H. Jenkins
Effects Of Feeding Field Peas In Combination With Distillers Grains Plus Solubles In Finishing And Growing Diets On Cattle Performance And Carcass Characteristics, A. C. Pesta, A. H. Titlow, J. A. Hansen, A. L. Berger, S. A. Furman, Matt K. Luebbe, G. E. Erickson, K. H. Jenkins
Department of Animal Science: Faculty Publications
Two studies were conducted to evaluate field peas and wet or dry corn distillers grains with solubles (WDGS and DDGS, respectively) in finishing and growing diets. In Exp. 1, British crossbred steers (n = 352, initial BW 356 ± 27 kg) were used in a randomized block design with factors being 0 or 20% field peas and 0 or 30% WDGS in dry-rolled corn (DRC) based finishing diets (DM basis). There was an interaction (P < 0.01) for DMI and G:F. Feeding WDGS increased ADG (P < 0.01), whereas peas had no effect on ADG (P = 0.33). Including WDGS increased G:F in diets without peas (P < 0.01), but had no impact (P = 0.12) in diets containing peas. Peas increased G:F (P = 0.04) in diets without WDGS, but decreased G:F (P = 0.03) with WDGS. Feeding WDGS increased HCW (P < 0.01). In Exp. 2, Continental crossbred heifers (yr. 1; n = 108, initial BW 338 ± 14 kg) and British crossbred steers (yr. 2; n = 90, initial BW 321 ± 10 kg) were assigned randomly to 1 of 9 pastures. Treatments were supplementation with loose DDGS meal on the ground (GROUND), in a bunk (BUNK) or a 25% field peas, 75% DDGS cube on the ground (CUBE) at equal CP. Final BW and ADG were less (P < 0.01) for GROUND than for CUBE and BUNK, which were similar. These data indicate up to 50% DRC could be replaced by peas and WDGS, and peas are an acceptable binder for DDGS range cubes.
Smooth Bromegrass Pasture Beef Growing Systems: Fertilization Strategies And Economic Analysis, A. K. Watson, T. J. Klopfenstein, Walter H. Schacht, G. E. Erickson, D. R. Mark, Matt K. Luebbe, K. R. Brink, Matthew A. Greenquist
Smooth Bromegrass Pasture Beef Growing Systems: Fertilization Strategies And Economic Analysis, A. K. Watson, T. J. Klopfenstein, Walter H. Schacht, G. E. Erickson, D. R. Mark, Matt K. Luebbe, K. R. Brink, Matthew A. Greenquist
Department of Animal Science: Faculty Publications
In recent years, prices for N fertilizer have increased dramatically, reducing net returns of fertilized pasture systems. A 5-yr study from 2005 to 2009 was conducted to evaluate management strategies and relative differences in profitability for 3 methods of backgrounding calves on smooth bromegrass pastures. Forty-five steers were used each year for a total of 225 animals in a randomized complete block design. Treatments included pastures fertilized in the spring with 90 kg N/ha (FERT), nonfertilized pastures with calves supplemented daily with dried distillers grains plus solubles (DDGS) at 0.6% of BW (SUPP), and control (CONT) pastures that had no …
Murine Gut Microbiota Is Defined By Host Genetics And Modulates Variation Of Metabolic Traits, Autumn M. Mcknite, Maria Elisa Perez-Munoz, Lu Lu, Evan G. Williams, Simon Brewer, Penelope A. Andreux, John W. M. Bastiaansen, Xusheng Wang, Stephen D. Kachman, Johan Auwerx, Robert W. Williams, Andrew K. Benson, Daniel A. Peterson, Daniel C. Ciobanu
Murine Gut Microbiota Is Defined By Host Genetics And Modulates Variation Of Metabolic Traits, Autumn M. Mcknite, Maria Elisa Perez-Munoz, Lu Lu, Evan G. Williams, Simon Brewer, Penelope A. Andreux, John W. M. Bastiaansen, Xusheng Wang, Stephen D. Kachman, Johan Auwerx, Robert W. Williams, Andrew K. Benson, Daniel A. Peterson, Daniel C. Ciobanu
Department of Animal Science: Faculty Publications
The gastrointestinal tract harbors a complex and diverse microbiota that has an important role in host metabolism. Microbial diversity is influenced by a combination of environmental and host genetic factors and is associated with several polygenic diseases. In this study we combined next-generation sequencing, genetic mapping, and a set of physiological traits of the BXD mouse population to explore genetic factors that explain differences in gut microbiota and its impact on metabolic traits. Molecular profiling of the gut microbiota revealed important quantitative differences in microbial composition among BXD strains. These differences in gut microbial composition are influenced by host-genetics, which …
Case Study: In Situ Determination Of Protein Digestibility Of Dried Distillers Grains Containing 3 Lipid Concentrations Using A Mobile Bag Method, B. E. Meyer, C. L. Bouma, James C. Macdonald
Case Study: In Situ Determination Of Protein Digestibility Of Dried Distillers Grains Containing 3 Lipid Concentrations Using A Mobile Bag Method, B. E. Meyer, C. L. Bouma, James C. Macdonald
Department of Animal Science: Faculty Publications
Ethanol producers remove lipid from distillers grains (DG) for applications such as biodiesel production. The effects of the lipid removal on ruminal protein degradability and total-tract CP digestibility of DG are not known. Five ruminally and duodenally cannulated Angus-cross steers (BW = 434 ± 15 kg) were used to incubate in situ bags for determination of protein digestibility of low-lipid (5.54%) DG, medium-lipid (8.40%) DG, high-lipid (12.46%) DG, and cottonseed meal. Ingredients were weighed into individual in situ bags and incubated in the ventral sac of the rumen for 16 h. After ruminal incubation and simulated abomasal digestion, bags were …
Dried Distillers Grains As A Substitute For Grazed Forage, L. A. Stalker, T. J. Klopfenstein, Walter H. Schacht, J. D. Volesky
Dried Distillers Grains As A Substitute For Grazed Forage, L. A. Stalker, T. J. Klopfenstein, Walter H. Schacht, J. D. Volesky
Department of Animal Science: Faculty Publications
A 2-yr study evaluated effects of feeding dried distillers grains (DDG) to yearlings grazing native range at greaterthan- recommended stocking rates on BW gain, grazed forage quality, and forage disappearance. Thirty-six paddocks were assigned randomly to 1 of 3 treatments: 1) control, stocked at a moderate stocking rate (1.48 animal unit months/ha in yr 1, 1.06 animal unit months/ha in yr 2) with no DDG; 2) double stocked, in which stocking rate was exactly twice the control with no DDG; and 3) double stocked with 2.27 kg/d (DM) of DDG per animal. Six paddocks per treatment replication were grazed in …
Technical Note: Estimating Beef-Cattle Forage Demand: Evaluating The Animal Unit Concept, T. L. Meyer, L. A. Stalker, J. D. Volesky, D. C. Adams, R. N. Funston, T. J. Klopfenstein, Walter H. Schacht
Technical Note: Estimating Beef-Cattle Forage Demand: Evaluating The Animal Unit Concept, T. L. Meyer, L. A. Stalker, J. D. Volesky, D. C. Adams, R. N. Funston, T. J. Klopfenstein, Walter H. Schacht
Department of Animal Science: Faculty Publications
The objective of this experiment was to evaluate the effect of BW and physiological status of a beef animal on forage intake. The experiment was repeated over 2 yr with 6 replications of 3 treatments per year: cow-calf pair (CCP, BW = 629 kg), nonlactating cow (NLC, BW = 503 kg), and yearling steer (YS, BW = 305 kg). The CCP was treated as one unit, with the sum of cow BW and calf BW comprising CCP BW. Calves averaged 42 d of age and 73 kg at the start of the experiment each year. Animals were housed in individual …
The Balance Of Proangiogenic And Antiangiogenic Vegfa Isoforms Regulate Follicle Development, Renee M. Mcfee, Timonthy G. Clopton, Andrea S. Cupp
The Balance Of Proangiogenic And Antiangiogenic Vegfa Isoforms Regulate Follicle Development, Renee M. Mcfee, Timonthy G. Clopton, Andrea S. Cupp
Department of Animal Science: Faculty Publications
Vascular endothelial growth factor A (VEGFA) has been extensively studied because of its role in follicular development and is a principal angiogenic factor essential for angiogenesis. Since vascularization of the theca layer increases as follicles progress in size through preantral and antral stages, VEGFA might influence follicle growth via the regulation of angiogenesis. However, VEGFA might also influence follicular development through nonangiogenicmechanisms, since its expression has been localized in nonvascular follicles and cells. Alternative mRNA splicing of eight exons from the VEGFA gene results in the formation of various VEGFA isoforms. Each isoform has unique properties and is identified by …
A High Density Snp Array For The Domestic Horse And Extant Perissodactyla: Utility For Association Mapping, Genetic Diversity, And Phylogeny Studies, Molly E. Mccue, Danika L. Bannasch, Jessica Lynn Petersen, Jessica Gurr, Ernie Bailey, Matthew M. Binns, Ottmar Distl, Gérard Guérin, Telhisa Hasegawa, Emmeline W. Hill, Tosso Leeb, Gabriella Lindgren, M. Cecilia T. Penedo, Knut H. Røed, Oliver A. Ryder, June E. Swinburne, Teruaki Tozaki, Stephanie J. Valberg, Mark Vaudin, Kerstin Lindblad-Toh, Claire M. Wade, James R. Mickelson
A High Density Snp Array For The Domestic Horse And Extant Perissodactyla: Utility For Association Mapping, Genetic Diversity, And Phylogeny Studies, Molly E. Mccue, Danika L. Bannasch, Jessica Lynn Petersen, Jessica Gurr, Ernie Bailey, Matthew M. Binns, Ottmar Distl, Gérard Guérin, Telhisa Hasegawa, Emmeline W. Hill, Tosso Leeb, Gabriella Lindgren, M. Cecilia T. Penedo, Knut H. Røed, Oliver A. Ryder, June E. Swinburne, Teruaki Tozaki, Stephanie J. Valberg, Mark Vaudin, Kerstin Lindblad-Toh, Claire M. Wade, James R. Mickelson
Department of Animal Science: Faculty Publications
An equine SNP genotyping array was developed and evaluated on a panel of samples representing 14 domestic horse breeds and 18 evolutionarily related species. More than 54,000 polymorphic SNPs provided an average inter-SNP spacing of ~43 kb. The mean minor allele frequency across domestic horse breeds was 0.23, and the number of polymorphic SNPs within breeds ranged from 43,287 to 52,085. Genome-wide linkage disequilibrium (LD) in most breeds declined rapidly over the first 50–100 kb and reached background levels within 1–2 Mb. The extent of LD and the level of inbreeding were highest in the Thoroughbred and lowest in the …