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Full-Text Articles in Life Sciences
Validation Of A New Rapid Hybridization Buffer For Fluorescence In Situ Hybridization With A Review On The Kinetics Of Dna Hybridization, Christine O'Connor
Validation Of A New Rapid Hybridization Buffer For Fluorescence In Situ Hybridization With A Review On The Kinetics Of Dna Hybridization, Christine O'Connor
Honors Scholar Theses
Abbott Molecular recently developed the rapid Vysis IntelliFISH Hybridization Buffer for use in fluorescence in situ hybridization (FISH) assays. The hybridization step in a standard FISH assay requires an overnight incubation, thus the turnaround time for a patient result is at least 24 hours. The IntelliFISH buffer was designed to reduce hybridization time of probes to target DNA to about two hours, allowing the entire assay to be performed in a single day. A review of methodologies and commercial products has highlighted the importance of hybridization kinetics to a successful FISH assay. The purpose of the research was to investigate …
Characterization Of Myod And Myf5 Double-Knockout Muscle Stem Cells During Muscle Development, Andreea Dinicu
Characterization Of Myod And Myf5 Double-Knockout Muscle Stem Cells During Muscle Development, Andreea Dinicu
University Scholar Projects
MyoD and Myf5 are transcription factors that regulate myogenesis by promoting satellite cell transcription. The two genes are known to display functional redundancy. Both genes are considered myogenic determination genes and are expressed in satellite cells. The fate of myogenic precursors in the absence of both MyoD and Myf5 remains largely unknown. We aimed to begin attaining this knowledge as part of this project. We utilized a CreLoxP system to control the expression of MyoD in mice lacking Myf5. MyoD was knocked out at embryonic day (E) 11.5 during myogenesis. Limbs were collected from experimental mice following tamoxifen injection. …