Life Sciences Commons^™

Analysis Of Genetic Diversity Using Simple Sequence Repeat (Ssr) Markers And Growth Regulator Response In Biofield Treated Cotton (Gossypium Hirsutum L.), Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Mahendra Kumar Trivedi

Cotton is the most important crop for the production of fiber that plays a key role in economic and social affairs. The aim of the study was to evaluate the impact of biofield energy treatment on cotton seeds regarding its growth, germination of seedling, glutathione (GSH) concentration, indole acetic acid (IAA) content and DNA fingerprinting using simple sequence repeat (SSR) markers for polymorphism analysis. The seeds of cotton cv. Stoneville-2 (Gossypium hirsutum L.) was obtained from DNA Land Marks Inc., Canada and divided into two groups. One group was remained as untreated, while the other was subjected to Mr. Trivedi …

Spectroscopic Characterization Of Chloramphenicol And Tetracycline: An Impact Of Biofield Treatment, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Objective: Chloramphenicol and tetracycline are broad-spectrum antibiotics and widely used against variety of microbial infections. Nowadays, several microbes have acquired resistance to chloramphenicol and tetracycline. The present study was aimed to evaluate the impact of biofield treatment for spectroscopic characterization of chloramphenicol and tetracycline using FT-IR and UV-Vis spectroscopy. Methods:The study was performed in two groups (control and treatment) of each antibiotic. The control groups remained as untreated, and biofield treatment was given to treatment groups. Results: FT-IR spectrum of treated chloramphenicol exhibited the decrease in wavenumber of NO2 from 1521 cm-1 to 1512 cm-1 and increase in wavenumber of …

An Effect Of Biofield Treatment On Multidrug-Resistant Burkholderia Cepacia: A Multihost Pathogen, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Burkholderia cepacia (B. cepacia) is an opportunistic, Gram negative pathogen which causes infection mainly in immunocompromised population and associated with high rate of morbidity and mortality in cystic fibrosis patients. Aim of the present study was to analyze the impact of biofield treatment on multidrug resistant B. cepacia. Clinical sample of B. cepacia was divided into two groups i.e. control and biofield treated. The analysis was done after 10 days of treatment and compared with control group. Control and treated group were analyzed for susceptibility pattern, MIC value, biochemical studies and biotype number using MicroScan Walk-Away® system.Sensitivity assay results showed …

Bio-Field Treatment: An Effective Strategy To Improve The Quality Of Beef Extract And Meat Infusion Powder, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

The present research work investigated the influence of bio-field treatment on two common flavoring agents used in food industries namely beef extract powder (BEP) and meat infusion powder (MIP). The treated powders were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), particle size analysis, surface area analysis, differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The FT-IR results showed disappearance of triglycerides peaks in both the treated powders as compared to control. XRD results corroborated the amorphous nature of both control and treated samples. The BEP showed enhanced average particle size (d50) and d99 (size exhibited by 99% …

Studies Of The Atomic And Crystalline Characteristics Of Ceramic Oxide Nano Powders After Bio Field Treatment, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Transition metal oxides (TMOs) have been known for their extraordinary electrical and magnetic properties. In the present study, some transition metal oxides (Zinc oxide, iron oxide and copper oxide) which are widely used in the fabrication of electronic devices were selected and subjected to biofield treatment. The atomic and crystal structures of TMOs were carefully studied by Fourier transform infrared (FT-IR) spectroscopy and X-ray diffraction (XRD) studies. XRD analysis reveals that biofield treatment significantly changed the lattice strain in unit cells, crystallite sizes and densities in ceramics oxide powders. The computed molecular weight of the treated samples exhibited significant variation. …

Ordinal Probit Wavelet-Based Functional Models For Eqtl Analysis, Mark J. Meyer, Jeffrey S. Morris, Craig P. Hersh, Jarret D. Morrow, Christoph Lange, Brent A. Coull

Jeffrey S. Morris

Current methods for conducting expression Quantitative Trait Loci (eQTL) analysis are limited in scope to a pairwise association testing between a single nucleotide polymorphism (SNPs) and expression probe set in a region around a gene of interest, thus ignoring the inherent between-SNP correlation. To determine association, p-values are then typically adjusted using Plug-in False Discovery Rate. As many SNPs are interrogated in the region and multiple probe-sets taken, the current approach requires the fitting of a large number of models. We propose to remedy this by introducing a flexible function-on-scalar regression that models the genome as a functional outcome. The …

Importance Of Genetic Diversity Assessment In Crop Plants And Its Recent Advances: An Overview Of Its Analytical Perspectives, Mahalingam Govindaraj

Mahalingam Govindaraj

The importance of plant genetic diversity (PGD) is now being recognized as a specific area since exploding population with urbanization and decreasing cultivable lands are the critical factors contributing to food insecurity in developing world.Agricultural scientists realized that PGD can be captured and stored in the formof plant genetic resources (PGR) such as gene bank,DNA library, and so forth, in the biorepository which preserve genetic material for long period. However, conserved PGR must be utilized for crop improvement in order to meet future global challenges in relation to food and nutritional security.This paper comprehensively reviews four important areas; (i) the …

Iniadi Pearl Millet Germplasm As A Valuable Genetic Resource For High Grain Iron, Mahalingam Govindaraj

Mahalingam Govindaraj

Crop biofortification is increasingly being recognized as a cost-effective and sustainable approach to address the widespread micronutrient malnutrition arising from Fe and Zn deficiencies. Pearl millet as a cereal crop species has higher Fe density than all other major cereals. Earlier studies in pearl millet have shown that breeding lines, hybrid parents, improved populations and composites having high Fe and Zn densities were often based largely or entirely on iniadi pearl millet germplasm. In an attempt to identify additional sources of high Fe density in this group of germplasm, 297 accessions were screened using Perl’s Prussian Blue staining, of which …

Cloning And Expression Analysis Of Drosophila Extracellular Cu Zn Superoxide Dismutase, Michael J. Blackney, Rebecca Cox, David Shepherd, Joel D. Parker

Joel D Parker

In the present study, we cloned and sequenced the mRNAs of the Sod3 [extracellular Cu Zn SOD (superoxide dismutase)] gene in Drosophila and identified two mRNA products formed by alternative splicing. These products code for a long and short protein derived from the four transcripts found in global expression studies (Flybase numbers Dmel\CG9027, FBgn0033631). Both mRNA process variants contain an extracellular signalling sequence, a region of high homology to the Sod1 (cytoplasmic Cu Zn SOD) including a conserved AUG start, with the longer form also containing a hydrophobic tail. The two fully processed transcripts are homologous to Caenorhabditis elegans Sod3 …

Pearl Millet Breeding Lines Developed At Icrisat:A Reservoir Of Variability And Useful Source, Mahalingam Govindaraj

Mahalingam Govindaraj

The International Crops Research Institute for the Semi- Arid Tropics (ICRISAT) conducts inter-disciplinary and partnership-based research for the genetic improvement of its mandate crops. During the 1970s and 1980s, the pearl millet improvement at ICRISAT, Patancheru, emphasized on: (i) developing a diverse range of traitspecific composites, based on the germplasm largely from the Western and Central Africa; (ii) improving them by the process of recurrent selection, principally for grain yield and downy mildew (Sclerospora graminicola (Sacc.) Schroet ) resistance; and (iii) developing open-pollinated varieties (OPVs)

The Empire Of Cancer: Gene Patents And Cancer Voices, Matthew Rimmer

Matthew Rimmer

In his book, The Emperor of All Maladies, Siddhartha Mukherjee writes a history of cancer — ‘It is a chronicle of an ancient disease — once a clandestine, “whispered-about” illness — that has metamorphosed into a lethal shape-shifting entity imbued with such penetrating metaphorical, medical, scientific, and political potency that cancer is often described as the defining plague of our generation’.Increasingly, an important theme in the history of cancer is the role of law, particularly in the field of intellectual property law. It is striking that a number of contemporary policy debates over intellectual property and public health have concerned …

Molecular Surveillance Of Low Pathogenic Avianinfluenza Viruses In Wild Birds Across The United States:Inferences From The Hemagglutinin Gene, Antoinette J. Piaggio, Susan A. Shriner, Kaci Van Dalen, Alan B. Franklin, Theodore D. Anderson, Sergios-Orestis Kolokotronis

Alan B Franklin

A United States interagency avian influenza surveillance plan was initiated in 2006 for early detection of highly pathogenic avian influenza viruses (HPAIV) in wild birds. The plan included a variety of wild bird sampling strategies including the testing of fecal samples from aquatic areas throughout the United States from April 2006 through December 2007. Although HPAIV was not detected through this surveillance effort we were able to obtain 759 fecal samples that were positive for low pathogenic avian influenza virus (LPAIV). We used 136 DNA sequences obtained from these samples along with samples from a public influenza sequence database for …

Democracia E Biotecnologia: Argumentos Para A Construção De Um Discurso Pautado Na Agência Humana Como Vetor Transformador Da Realidade, Carolina Altoé Velasco

Carolina Altoé Velasco

O artigo objetiva demonstrar que os processos biotecnológico e democrático têm em comum a interferência da agência humana como vetor transformador de suas realidades. Utiliza-se como marco teórico-metodológico as obras de Guillermo O’Donnell e Adela Cortina. Guillermo O’Donnell reconhece o impacto motivado pela tecnologia e globalização na sociedade e a agência humana como fomentadora do processo democrático. Já Adela Cortina considera a pessoa (contemplada por sujeito autônomo e solidário) a medida da democracia e esta como forma de vida. A eleição de Cortina para travar um diálogo com o pensamento de O’Donnell se dá em razão da abordagem feita a …

Impact Of Biofield Treatment On Growth And Yieldof Lettuce And Tomato, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Recent studies report the effect of biofield treatment on changes in structural characteristics of organic and inorganic matter, on cancer cells in vitro and on overall plant development. This study tested the impact of the same treatment applied to lettuce and tomato seeds and transplants (Lactuca sativa var. capitata and Lycopersiconesculentum var. Roma) in commercial plantings with and without fertilizers and pesticides, in relation to yield, quality, and pest inhibition. Treated lettuce plants with fertilizer and pesticide applications were more vigorous, exhibited less incidence of soil-borne fungal wilt, and subsequent yield was statistically greater 43% compared to untreated plants. Treated …

Impact Of Biofield Treatment On Growth And Anatomical Characteristics Of Pogostemoncablin (Benth.), Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Pogostemoncablin is a known aromatic plant which is cultivated for its essential oil widely applicated in perfumery and cosmetic industries. In the present study, the effect of biofield treatment was studied on the growth of P. cablin. For this study an in-vitro culture system was set up in two groups, viz., control and treatment, each of which was derived from three different explant sources, namely leaf, node and petiole. Further these in-vitro plantlets were hardened and transferred to external environment. The stomatal cells and epidermal hair growth were also studied at various morphogenetic stages. The study revealed that a single …

Lack Of Association Between Gly82ser, 1704g/T And 2184a/G Of Rage Gene Polymorphisms And Retinopathy Susceptibility In Malaysian Diabetic Patients, Rozaida @ Poh Yuen Ying

Rozaida @ Poh Yuen Ying

Diabetic retinopathy is the most common diabetic eye disease, occurring in about 60% of type 2 diabetic patients. Other than known clinical risk factors, the influence of genes has been suggested as part of the development of diabetic retinopathy. We investigated the association of Gly82Ser, 1704G/T and 2184A/G polymorphisms in the RAGE gene with retinopathy in type 2 diabetic patients in Malaysia. Ninety-eight unrelated retinopathy patients and 185 unrelated healthy controls from all over Malaysia were recruited in this study. The allele and genotype frequencies of the three gene polymorphisms were investigated using PCR-RFLP. The allele frequency of the three …

Igf2bp2 Alternative Variants Associated With Glutamic Acid Decarboxylase Antibodies Negative Diabetes In Malaysian Subjects, Rozaida @ Poh Yuen Ying

Rozaida @ Poh Yuen Ying

Background: The association of Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) common variants (rs4402960 and rs1470579) with type 2 diabetes (T2D) has been performed in different populations. The aim of this study was to evaluate the association of alternative variants of IGF2BP2; rs6777038, rs16860234 and rs7651090 with glutamic acid decarboxylase antibodies (GADA) negative diabetes in Malaysian Subjects. Methods/Principal Findings: IGF2BP2; rs6777038, rs16860234 and rs7651090 single nucleotide polymorphisms (SNPs) were genotyped in 1107 GADA negative diabetic patients and 620 control subjects of Asian from Malaysia. The additive genetic model adjusted for age, race, gender and BMI showed that alternative variants; …

Paraoxonase 1 Status In Keratoconus: A Preliminary Study Of Activity And Polymorphism, Rozaida @ Poh Yuen Ying

Rozaida @ Poh Yuen Ying

OBJECTIVE: To determine the activity of paraoxonase 1 (PON1) in keratoconus in a Malaysian population in comparison with non-keratoconic subjects. METHODS: Clinical eye examinations were performed on patients with keratoconus and non-keratoconic subjects after questionnaires were completed. Blood samples were collected and subjected to spectrophotometry analysis of paraoxonase and diazoxonase activities for the determination of the status of PON1 of every individual. RESULTS: Of the 11 keratoconic patients and 55 non-keratoconic control samples collected, eight patients of Indian ethnicity were keratoconic (73%), whereas 33 non-Indians were non-keratoconic (60%; p = 0.047). Paraoxonase activity was lower in Indians compared to the …

The Use Of Imputed Values In The Meta-Analysis Of Genome-Wide Association Studies., Shuo Jiao, Li Hsu, Carolyn Hutter, Ulrike Peters

Shuo Jiao

In genome-wide association studies (GWAS), it is a common practice to impute the genotypes of untyped single nucleotide polymorphism (SNP) by exploiting the linkage disequilibrium structure among SNPs. The use of imputed genotypes improves genome coverage and makes it possible to perform meta-analysis combining results from studies genotyped on different platforms. A popular way of using imputed data is the "expectation-substitution" method, which treats the imputed dosage as if it were the true genotype. In current practice, the estimates given by the expectation-substitution method are usually combined using inverse variance weighting (IVM) scheme in meta-analysis. However, the IVM is not …

Phenology Of Stable Fly (Diptera: Muscidae) Larvae In Round Bale Hay Feeding Sites In Eastern Nebraska., David B. Taylor, Dennis R. Berkebile

David B Taylor

The temporal and spatial patterns of adult stable fly, Stomoxys calcitrans (L.), emergence from six sites where large round baled hay had been provided to pasture cattle as winter feed were studied using emergence traps. The substrate at these sites, consisting of waste hay mixed with bovine manure and urine, provided an excellent developmental habitat for immature stable flies. Stable flies were the most frequently collected fly emerging from these sites with a yearly average of 1,581 emerging per square meter. Stable fly emergence from these sites began in early May (235 annual accumulated Day-Degree 10° C [DD10]), peaked in …

Paraoxonase 1 Activity As A Predictor Of Cardiovascular Disease In Type 2 Diabetes, Rozaida @ Poh Yuen Ying

Rozaida @ Poh Yuen Ying

The role of paraoxonase 1 in cardiovascular disease complications in type 2 diabetes mellitus is not fully understood. We studied paraoxonase activity towards paraoxon in 188 non-diabetic and 140 diabetic subjects using general linear models and univariate analysis. Adjusting for age revealed a reduction in activity towards paraoxon was associated with a significant increase in risk (p=0.023) for cardiovascular disease complications in diabetic patients. Multivariate analysis of two plasma measures of paraoxonase activity using paraoxon and diazoxon also showed reduced paraoxonase activity towards paraoxon was associated with a significant increase in risk (p=0.045) for cardiovascular disease complications in diabetic patients. …

Dispersal Of Stable Flies (Diptera: Muscidae) From Larval Developmental Sites., David B. Taylor, Roger D. Moon, Jack B. Campbell, Dennis R. Berkebile, Philip J. Scholl, Alberto B. Broce, Jerome A. Hogsette

David B Taylor

Seven mark-recapture studies were conducted over 3 yr to assess dispersal of newly emerging adult stable flies, Stomoxys calcitrans L., from larval development sites in a mixed agricultural environment in northeastern Nebraska. Infested hay debris piles were marked by dusting their surfaces with fluorescent pigments, adults were captured with surrounding grids of Alsynite sticky traps, and specimens were dissected to determine feeding histories and reproductive age. Distances and directions of 3,889 marked specimens indicated males and females dispersed equally and in all directions. Midguts of males and females were equally likely to contain blood-meal remnants. Percentage with blood remnants and …

Effect Of Superconsciousness External Energy On The Atomic, Crystalline And Powder Characterisitics Of “Carbon Allotrope Powders”, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Scientists are searching for eluding link between spirituality and science. Some believe fundamental essences of universe to be energy and information. As per current understanding energy and matter always co- existed and is considered one and the same. Energy is consideredas ‘matter-inperpetual-motion’and matter as ‘stationary-energy’.Inter conversion between matter and energy has been defined by Einstein’s famous energy-mass equation (E=mc2) which has been provedby nuclear physicists using complex nuclear reactions involving high energy particles. However, many spiritual masters have claimed to realize this energy-matter inter conversion using their spiritual powers/energy but scientifically unknown and unverified. It is the first time that …

Genetic Effect Of The Dwarfing Genes On Some Culm Characteristics Associatcd With Lodging Resistance In Bread Wheat, Md. Mahbub Hasan

Md. Mahbub Hasan

Due to the challenge of screening traits related to lodging resistance under natural field conditions, selection for lodging resistant varieties in wheat breeding programs is difficult. The identification of easily measurable culm anatomical traits related to lodging resistance would simplify the selection process. The present study was conducted to determine the effect of dwarfing genes on culm anatomical traits related to lodging resistance in our of basal internode 1. Field and laboratory study was conducted in Shahjalal University of Science and Technology, Sylhet, Bangladesh with eight wheat genotypes having Rhr1, Rht2 dwarfing genes in them and a local land race …

Ppar Agonists Down-Regulate The Expression Of Atp10c Mrna During Adipogenesis, A Peretich, Maria Cekanova Ms, Rndr, Phd, S Hurst, Sj Baek, Madhu Dahr

Maria Cekanova MS, RNDr, PhD

No abstract provided.

Impact Of An External Energy On Enterococcus Faecalis [Atcc – 51299] In Relation To Antibiotic Susceptibility And Biochemical Reactions – An Experimental Study, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Background:

While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Enterococcus faecalis [ATCC –51299], report the effects of such energy transmitted through a person, Mahendra Trivedi, which has produced an impact measurable in scientifically rigorous manner.

Methods:

Enterococcus faecalis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain, the process taking about 3 minutes and were analyzed within 10 …

Evaluation Of Polymorphism At Codon 192 Of Paraoxonase 1 On Its Kinetic Behavior, Rozaida @ Poh Yuen Ying

Rozaida @ Poh Yuen Ying

Human paraoxonase 1 (PON1), a High-Density Lipoprotein (HDL)-associated esterase has been implicated in slowing down the development of atherosclerosis. In the present study, kinetic and inhibition studies on PON1 were conducted to assess three parameters: the Michaelis constant (KM) and maximal rate of metabolisme (Vmax) of paraoxonase and inhibition constant (Ki) of phenylacetate. Human paraoxonase 1 (PON1) activity was measured spectrophotometrically at 405 nm, using plasma samples in basal (without added NaCl) and salt-stimulated assays with 1 M NaCl. Inhibition studies were performed using phenylacetate as an inhibitor of PON1 in basal assays, pH 8.0. Estimates of KM and Vmax …

A New Method For Collecting Clean Stable Fly (Diptera: Muscidae) Pupae Of Known Age., Dennis R. Berkebile, Anthony P. Weinhold, David B. Taylor

David B Taylor

Stable flies, Stomoxys calcitrans L., are important pests of confined and pasture cattle. They have been reared in the laboratory to study their biology and to test new methods of control. Research on rearing modifications has concentrated on developing larval diets from materials locally abundant. Under current protocols, pupae form in the medium. Aggregations of pupae were located and removed, often with a considerable amount of extraneous material. Various methods have been developed to separate the pupae from waste material. We describe a method by which wandering larvae are enticed to leave the medium prior to pupariation. The larvae were …

Impact Of An External Energy On Staphylococcus Epidermis [Atcc –13518] In Relation To Antibiotic Susceptibility And Biochemical Reactions – An Experimental Study, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Purpose:

While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiment on Staphylococcus epidermis [ATCC –13518], validate the effects of such energy transmitted through a person, Mahendra Trivedi, which has produced an impact measurable in scientifically rigorous manner.

Methods:

Staphylococcus epidermis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mahendra Trivedi to the sealed tubes containing strain, the process taking about 3 minutes and were analyzed within 10 …

Using The Estimated Penetrances To Determine The Range Of The Underlying Genetic Model In Case-Control Design, Mark J. Meyer, Neal Jeffries, Gang Zheng

Mark J Meyer

It is well known that the penetrance cannot be estimated using the retrospective case- control samples without making additional assumptions. In the literature the estimation of the penetrance is based on the assumptions that either the disease is rare or the disease prevalence is known. We propose an alternative approach to estimate the penetrance by assuming an underlying genetic model even though it is unknown. With this assumption, we can obtain the point estimates of the penetrances as functions of the genetic model, from which the range of underlying genetic models can be determined. We examine the performance of our …