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Identification Of Three Randomly Amplified Polymorphic Dna-Polymerase Chain Reaction Markers For Distinguishing Asian And North American Gypsy Moths (Lepidoptera: Lymantriidae), David E. Schreiber, Karen J. Garner, James M. Slavicek
Identification Of Three Randomly Amplified Polymorphic Dna-Polymerase Chain Reaction Markers For Distinguishing Asian And North American Gypsy Moths (Lepidoptera: Lymantriidae), David E. Schreiber, Karen J. Garner, James M. Slavicek
USDA Forest Service / UNL Faculty Publications
Gypsy moths originating in Asia have recently been introduced into North America, making it necessary to develop markers for distinguishing the Asian strain from the established North American population. We have identified 3 randomly amplified polymorphic DNA-polymerase chain reaction generated (RAPD-PCR) markers which are specific for either Asian or North American moths. The FS-2 and FS-3 markers are specific for the North American strain, and the FS-4 marker is specific for moths from far east Asia. The 3 RAPD-amplified fragments have been cloned and sequenced, and locus-specific primers have been developed for the FS-3 marker. These markers, in combination with …