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Full-Text Articles in Life Sciences
Two High Hierarchical Regulators, Pumyb40 And Puwrky75, Control The Low Phosphorus Driven Adventitious Root Formation In Populus Ussuriensis, Hanzeng Wang, Solme Pak, Jia Yang, Ye Wu, Wenlong Li, He Feng, Jingli Yang, Hairong Wei, Chenghao Li
Two High Hierarchical Regulators, Pumyb40 And Puwrky75, Control The Low Phosphorus Driven Adventitious Root Formation In Populus Ussuriensis, Hanzeng Wang, Solme Pak, Jia Yang, Ye Wu, Wenlong Li, He Feng, Jingli Yang, Hairong Wei, Chenghao Li
Michigan Tech Publications
Adventitious rooting is an essential biological process in the vegetative propagation of economically important horticultural and forest tree species. It enables utilization of the elite genotypes in breeding programmes and production. Promotion of adventitious root (AR) formation has been associated with starvation of inorganic phosphate and some factors involved in low phosphorus (LP) signalling. However, the regulatory mechanism underlying LP-mediated AR formation remains largely elusive. We established an efficient experimental system that guaranteed AR formation through short-term LP treatment in Populus ussuriensis. We then generated a time-course RNA-seq data set to recognize key regulatory genes and regulatory cascades positively regulating …
A Systems Biology Approach Identifies A Regulator, Bplerf1, Of Cold Tolerance In Betula Platyphylla, Kaiwen Lv, Wenqi Wu, Hairong Wei, Guifeng Liu
A Systems Biology Approach Identifies A Regulator, Bplerf1, Of Cold Tolerance In Betula Platyphylla, Kaiwen Lv, Wenqi Wu, Hairong Wei, Guifeng Liu
Michigan Tech Publications
Cold is an abiotic stress that can greatly affect the growth and survival of plants. Here, we reported that an AP2/ERF family gene, BplERF1, isolated from Betula platyphylla played a contributing role in cold stress tolerance. Overexpression of BplERF1 in B. platyphylla transgenic lines enhanced cold stress tolerance by increasing the scavenging capability and reducing H2O2 and malondialdehyde (MDA) content in transgenic plants. Construction of BplERF-mediated multilayered hierarchical gene regulatory network (ML-hGRN), using Top-down GGM algorithm and the transcriptomic data of BplERF1 overexpression lines, led to the identification of five candidate target genes of BplERF1 which include MPK20, …
Growth-Regulating Factor 5 (Grf5)-Mediated Gene Regulatory Network Promotes Leaf Growth And Expansion In Poplar, Wenqi Wu, Jiang Li, Qiao Wang, Kaiwen Lv, Kang Du, Hairong Wei
Growth-Regulating Factor 5 (Grf5)-Mediated Gene Regulatory Network Promotes Leaf Growth And Expansion In Poplar, Wenqi Wu, Jiang Li, Qiao Wang, Kaiwen Lv, Kang Du, Hairong Wei
Michigan Tech Publications
Although polyploid plants have larger leaves than their diploid counterparts, the molecular mechanisms underlying this difference (or trait) remain elusive. Differentially expressed genes (DEGs) between triploid and full-sib diploid poplar trees were identified from two transcriptomic data sets followed by a gene association study among DEGs to identify key leaf growth regulators. Yeast one-hybrid system, electrophoretic mobility shift assay, and dual-luciferase assay were employed to substantiate that PpnGRF5-1 directly regulated PpnCKX1. The interactions between PpnGRF5-1 and growth-regulating factor (GRF)-interacting factors (GIFs) were experimentally validated and a multilayered hierarchical regulatory network (ML-hGRN)-mediated by PpnGRF5-1 was constructed with top-down graphic Gaussian model …