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Michigan Tech Publications

Betula platyphylla

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A Systems Biology Approach Identifies A Regulator, Bplerf1, Of Cold Tolerance In Betula Platyphylla, Kaiwen Lv, Wenqi Wu, Hairong Wei, Guifeng Liu Jun 2021

A Systems Biology Approach Identifies A Regulator, Bplerf1, Of Cold Tolerance In Betula Platyphylla, Kaiwen Lv, Wenqi Wu, Hairong Wei, Guifeng Liu

Michigan Tech Publications

Cold is an abiotic stress that can greatly affect the growth and survival of plants. Here, we reported that an AP2/ERF family gene, BplERF1, isolated from Betula platyphylla played a contributing role in cold stress tolerance. Overexpression of BplERF1 in B. platyphylla transgenic lines enhanced cold stress tolerance by increasing the scavenging capability and reducing H2O2 and malondialdehyde (MDA) content in transgenic plants. Construction of BplERF-mediated multilayered hierarchical gene regulatory network (ML-hGRN), using Top-down GGM algorithm and the transcriptomic data of BplERF1 overexpression lines, led to the identification of five candidate target genes of BplERF1 which include MPK20, …


Overexpression Of Bplerd15 Enhances Drought Tolerance In Betula Platyphylla Suk, Kaiwen Lv, Hairong Wei, Jing Jiang Sep 2020

Overexpression Of Bplerd15 Enhances Drought Tolerance In Betula Platyphylla Suk, Kaiwen Lv, Hairong Wei, Jing Jiang

Michigan Tech Publications

In this study, we report the cloning and functional characterization of an early responsive gene, BplERD15, from Betula platyphylla Suk to dehydration. BplERD15 is located in the same branch as Morus indica Linnaeus ERD15 and Arabidopsis Heynh ERD15 in the phylogenetic tree built with ERD family protein sequences. The tissue-specific expression patterns of BplERD15 were characterized using qRT-PCR and the results showed that the transcript levels of BplERD15 in six tissues were ranked from the highest to the lowest levels as the following: mature leaves (ML) > young leaves (YL) > roots (R) >buds (B) >young stems (YS) >mature stems (MS). Multiple …