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Full-Text Articles in Life Sciences

Application Of Short Tandem Target Mimic (Sttm) Technique For Functional Analysis Of Micro-Rna396 In Transgenic Poplar Trees, Surattana Boonsai Jan 2019

Application Of Short Tandem Target Mimic (Sttm) Technique For Functional Analysis Of Micro-Rna396 In Transgenic Poplar Trees, Surattana Boonsai

Dissertations, Master's Theses and Master's Reports

Short Tandem Target Mimic (STTM) is a commonly technique used for functional studies of a number of genes in several plant model systems. However, very little is known about application of STTM technique in tree species. In this study, STTM was applied to knock down microRNA396 (miR396) in transgenic poplar trees for the first time. STTM396 expression resulted in dramatic decrease in miR396 expression levels leading to taller plants with larger leaves and larger leaf cell size. Additionally, an expression analysis of growth regulating factor genes (GRFs) that are members of miR396 target gene family showed up-regulation of GRF07 gene …


Plant Community Response To Novel Silvicultural Treatments In Great Lakes Northern Hardwoods, Stefan F. Hupperts Jan 2019

Plant Community Response To Novel Silvicultural Treatments In Great Lakes Northern Hardwoods, Stefan F. Hupperts

Dissertations, Master's Theses and Master's Reports

The objective of this dissertation is to assess plant community response across a range of silvicultural disturbances and test ecological hypotheses to better inform ecologists and forest managers. To provide context for the utility of revising silvicultural systems, I review natural disturbance regimes and historical practices that have shaped contemporary Great Lakes northern hardwood forests (Chapter 2). Further, I identify important ways to expand the silvicultural toolbox and better emulate natural disturbance regimes. Building on this theoretical underpinning, I investigate the initial regeneration and plant community response to two novel silvicultural experiments: the Northern Hardwood Experiment for Enhancing Diversity (NHSEED) …


Spatiotemporal Variability In Winter Severity: Consequences For White-Tailed Deer Populations And Habitat Sustainability, Grace Parikh Jan 2019

Spatiotemporal Variability In Winter Severity: Consequences For White-Tailed Deer Populations And Habitat Sustainability, Grace Parikh

Dissertations, Master's Theses and Master's Reports

Winter in the northern Great Lakes presents a suite of challenging conditions for animals, in terms of limited food availability and increased energetic cost of locomotion and thermoregulation. Variable winter severity is liable to cause interannual fluctuations in habitat viability and use by animals, in addition to modulating physiological responses in animals to conserve energy. For example, white-tailed deer (Odocoileus virginianus) congregate at high densities under eastern hemlock (Tsuga canadensis) or northern white-cedar (Thuja occidentalis) stands, which provide forage, thermal cover, reduced snow depth, and enhanced vigilance. However, a suite of climatic, edaphic, and …