Life Sciences Commons^™

Therapeutic Effects Of Orally Administration Of Viable And Inactivated Probiotic Strains Against Murine Urinary Tract Infection, Vo Thi Hong Van, Zhen-Shu Liu, Yueh Jen Hsieh, Wei-Chen Shiu, Bo-Yuan Chen, Yu-We Ku, Po-Wen Chen

Journal of Food and Drug Analysis

Urinary tract infections (UTIs) are highly prevalent bacterial infections that pose significant health risks. Specific probiotic strains have been recommended for UTI control and management of antibiotic resistance. Otherwise, paraprobiotics, defined as inactivated probiotic cells, offer potential advantages by minimizing risks associated with live microorganisms. However, the effectiveness of heat-killed probiotic strains against UTIs remains uncertain. Additionally, lactoferrin (LF), an iron-binding glycoprotein, exhibits immunomodulatory, antimicrobial, and anti-inflammatory properties. Recently, we had developed recombinant LF-expression probiotics, which can display considerate antibacterial activities against select food-borne pathogens in vitro. Thus, the present study aimed to evaluate the antibacterial activities of heat-killed natural …

Study On The Preparation Of Ace Inhibitory Peptides By Probiotic Fermentation Of Quinoa, Li Shuai, Yuan Ya-Hong, Yue Tian-Li

Food and Machinery

Objective: The potential ability of probiotics to prepare angiotensin-converting enzyme (ACE) inhibitory fermentation broth from quinoa and the effects of fermentation process on it were investigated. Methods: In this study, 18 strains of lactic acid bacteria and 12 strains of yeast were screened for their ability to prepare ACE inhibitory fermentation broth using quinoa as the raw material and the ACE inhibition rate as an indicator. The inoculum amount, fermentation temperature and fermentation time were optimized by orthogonal tests. Finally, the peptides in the fermentation broth were identified, and the selected peptides were tested in vivo to evaluate …

The Future Of Synbiotics: Rational Formulation And Design, David Fabian Gomez Gomez, Car Reen Kok, Robert Hutkins

Department of Food Science and Technology: Faculty Publications

Synbiotics, mixtures of live microbes and substrates selectively utilized by host organisms, are of considerable interest due to their ability to improve gastrointestinal health. However, formulating synbiotics remains challenging, due in part, to the absence of rational strategies to assess these products for synbiotic activities prior to clinical trials. Currently, synbiotics are formulated as either complementary or synergistic. Complementary synbiotics are made by combining probiotics and prebiotics, with each component acting independently and with the combination shown to provide a clinical health benefit. Most commercial synbiotics as well as those used in clinical trials have been of the complementary type. …

Characterization Of Clostridium Cochlearium As A Potential Probiotic For Obesity Management, Paba Edirisuriya

Wayne State University Dissertations

ABSTRACTCHARACTERIZATION OF CLOSTRIDIUM COCHLEARIUM AS A POTENTIAL PROBIOTIC FOR OBESITY MANAGEMENT

ByPABA EDIRISURIYA MAY 2021 Advisor: Dr. Kequan Zhou Major: Nutrition and food science Degree: Doctor of Philosophy Emerging evidence indicates that manipulation of gut microflora is a potential therapeutic approach for managing obesity. Probiotic effects on host weight reduction have repeatedly been revealed through previous studies. Clostridium cochlearium is a butyrate-producing, spore-forming bacteria that have been reported to present in the mammalian gut. Our simulated Invitro digestion model revealed that C. cochlearium could survive in the unfavorable conditions of the human gastrointestinal tract, including low pH (pH2), high bile …

Influence Of Health Beneficial Monk Fruit Sweetener On Microbial And Physicochemical Characteristics Of Camel Milk Yogurt, Alina Buchilina

LSU Master's Theses

Camel milk contains all the essential nutrients found in cow’s milk and has potentially health beneficial compounds such as anti-carcinogenic, anti-hypertensive, anti-inflammatory, and insulin and insulin-like substances. Camel milk has been reported as a treatment for diseases such as diabetes and autism. Camel milk favorably alters gut microbiota to a higher abundance of bifidobacteria.

Sweetness is one of the most desirable tastes in people’s diet; however, the surplus consumption of sugar has a negative effect on human health. Monk fruit sweetener is a natural zero caloric sweetener with many health beneficial properties, namely, prevention of asthma, prevention of oxidation, liver …

Effect Of Supplemental Prebiotics, Probiotics And Bioactive Proteins On The Microbiome Composition And Gut Physiology In C57bl6/J Mice, Ye Li

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The composition and metabolic activity of the microbiome affect many aspects of health, and there is current interest in dietary constituents that may affect this system. The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice individually and in combination. Mice were fed the total western diet (TWD) supplemented with prebiotics, probiotics, and Tri-Factor (bioactive proteins) individually and in combination for four weeks. Subsequently, effects on the composition of gut microbiome, gut short chain fatty acids (SCFAs) concentration, gut …

Probiotic Characterization Of Lactic Acid Bacteria (Lab) Isolated From Dried Emirati Fish And The Health Promoting Benefits Of Fermented Fish Sausages By Selected Isolates, Nadya Salem Sultan Saeed Al Kalbani

Food Science Theses

Probiotics have great positive impacts on human health. Therefore, finding new probiotics possess novel probiotic characteristics would be greatly appreciated by medical, scientific and food industrial societies. This study aimed to isolate lactic acid bacteria (LAB) from traditionally dried fishes, assess their safety aspects, characterize their probiotic features, and investigate their potential health-promoting benefits of fish sausage fermented by selected LABs, in-vitro.

Out of one hundred and fifty (150) isolates, 85 isolates were considered as a potential probiotic. The 85 isolates achieved a reduction under gastric juicy ranged from 0.5-5.2 log10 CFU/mL during 2h of incubation and they were …

Probiotic Bifidobacterium Strains And Galactooligosaccharides Improve Intestinal Barrier Function In Obese Adults But Show No Synergism When Used Together As Synbiotics, Janina A. Krumbeck, Heather E. Rasmussen, Robert W. Hutkins, Jennifer L. Clarke, Krista Shawron, Ali Keshavarzian, Jens Walter

Department of Food Science and Technology: Faculty Publications

Background: One way to improve both the ecological performance and functionality of probiotic bacteria is by combining them with a prebiotic in the form of a synbiotic. However, the degree to which such synbiotic formulations improve probiotic strain functionality in humans has not been tested systematically. Our goal was to use a randomized, double-blind, placebo-controlled, parallel-arm clinical trial in obese humans to compare the ecological and physiological impact of the prebiotic galactooligosaccharides (GOS) and the probiotic strains Bifidobacterium adolescentis IVS-1 (autochthonous and selected via in vivo selection) and Bifidobacterium lactis BB-12 (commercial probiotic allochthonous to the human gut) when used …

Synbiotic Ice Cream As A Probiotic Carrier Tested In A Human Blind Crossover Trial, Erin M. Mcnamara

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Diet is an important factor in gastrointestinal health. A synbiotic food option utilizing prebiotic and probiotic ingredients may be beneficial for improving gastrointestinal health. To conduct a human subject study of synbiotic ice cream containing prebiotic (inulin) and two strains of probiotics (Lactobacillus casei KE99 and Bifidobacterium bifidum) to determine its effectiveness as a carrier for a these ingredients and to identify any negative gastrointestinal side effects.

The study started with baseline data collection including a food frequency, a three day food recall, and one stool sample. The study was a 12 week crossover design with three weeks consuming …

Efficacy Of Galactooliosaccharide (Gos) And/Or Rhamnose-Based Synbiotics In Enhancing Ecological Performance Of Lactobacillus Reuteri In The Human Gut And Characterization Of Its Gos Metabolic System, Monchaya Rattanaprasert

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Probiotic L. reuteri has potential to produce antimicrobial compounds and secrete immunosuppressive factors. These metabolic attributes could benefit the human host by providing colonization resistance (competitive and metabolic exclusion) against enteropathogens and mitigating inflammation. As metabolically active cells are fundamental to such probiotic properties, synbiotic approaches that supply L. reuteri with a source(s) of carbon, energy, and/or external electron acceptor for cell growth in the gut environment could therefore prompt the probiotic to engage in beneficial activities. In this study, the efficacy of GOS and/or rhamnose-based synbiotic approaches in promoting colonization persistence and metabolic activity of L. reuteri was evaluated. …

Non-Dairy Probiotic Products, Nissreen Abu-Ghannam, Gaurav Rajauria

Books/Book Chapters/ Proceedings

The term probiotic was technically defined as “live microorganisms which upon ingestion in certain numbers exert health benefits beyond inherent nutrition” (FAO/ WHO 2001). This definition requires that the microorganisms must be alive and present in high numbers, generally more than 109 cells per daily ingested dose. Probiotic food products are considered as functional foods which are defined to contain health-promoting components beyond traditional nutrients and the addition of probiotic cultures is one approach in which foods could be modified to become functional.

Physicochemical Properties, Microstructure And Probiotic Survivability Of Non-Fat Goat's Milk Yogurt Using Heat Treated Whey Protein Concentrate As A Fat Replacer, James Thomas Mccarthy

Graduate College Dissertations and Theses

Probiotic dairy foods, especially non- and low-fat dairy products, are becoming popular in the US. A non-fat goat's milk yogurt containing probiotics (Lactobacillus acidophilus and Bifidobacterium spp.) was developed using heat-treated whey protein concentrate (HWPC) as a fat replacer and pectin as a thickening agent. Yogurts containing non-heat treated whey protein concentrate (WPC) and pectin as well as one with only pectin were also produced. A fat-free cow's milk yogurt with pectin was also used as a control yogurt. The yogurts were analyzed for chemical composition, water holding capacity (syneresis), microstructure, changes in pH and viscosity, mold, yeast and coliform …

Survival Of Lactobacillus Acidophilus And Bifidobacteria Bifidum In Ice Cream For Use As A Probiotic Food, Sharareh Hekmat

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ice cream mix (12% fat, 11% milk solids nonfat, 12.5% sugar, and 4.5% corn syrup solids) was fermented with Lactobacillus acidophilus and Bifidobacterium bifidum. Half of the mix was heat treated at 82°C for 30 minutes and cooled to 40-41°C. The other half was warmed to 40-41°C and inoculated with the starter cultures. Both were made into ice cream and stored at -29°C. Survival of L. acidophilus and B. bifidum and of ẞ-galactosidase activity were monitored during 17 weeks of frozen storage. Reinforced clostridial medium was used to enumerate culture bacteria. Colony counts, after fermentation, for both L. acidophilus …