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Full-Text Articles in Life Sciences
Inactivation Of Escherichia Coli O157:H7 And Shiga Toxin Producing E. Coli (Stec) Throughout Beef Summer Sausage Production And The Use Of High Pressure Processing As An Alternative Intervention To Thermal Processing, Eric L. Oliver
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Escherichia coli O157:H7 and six additional serogroups of Shiga Toxin-producing E. coli (STEC-7) have been implicated in foodborne illness outbreaks related to fermented sausage products and are considered adulterants by the USDA-FSIS. The objective of this study was to evaluate high pressure processing (HPP) as an alternative to thermal treatment to reduce STEC-7 meeting the USDA-FSIS performance standards (5.0-log reduction). A rifampicin-resistant, non-pathogenic E. coli (surrogate) cocktail, was used in manufacturing testing. Sausages were fermented at ~42.2°C, slowly increased to 54.4°C internal temperature, cold showered, and chilled for 6 hours. Sampling occurred post-fermentation (PF; pH 5.0), internal temperature 48.9°C (I-48.9°C), …
The Influence Of The Bovine Fecal Microbiota On The Shedding Of Shiga Toxin-Producing Escherichia Coli (Stec) By Beef Cattle, Nirosh D. Aluthge
The Influence Of The Bovine Fecal Microbiota On The Shedding Of Shiga Toxin-Producing Escherichia Coli (Stec) By Beef Cattle, Nirosh D. Aluthge
Department of Food Science and Technology: Dissertations, Theses, and Student Research
During the past three decades, Shiga toxin-producing E.coli (STEC) have emerged as an important food safety concern. Although initially E. coli O157 was the main focus, recent outbreaks and resulting investigations have shown that certain non-O157 STEC are as much a threat to food safety as their O157 counterparts. To the beef industry, STEC have been of particular concern due to the frequent association of beef and beef products as vehicles of STEC infection. As a result, along with E. coli O157, six non-O157 STEC serogroups (known as the ‘big six’) are now regulated as adulterants in certain raw beef …
Shiga Toxin-Producing Escherichia Coli In Meat: A Preliminary Simulation Study On Detection Capabilities For Three Sampling Methods, Julie Couton, David Marx, John Luchaansky, Randall Phebus, Anna Porto-Fett, Nicholas Sevart, Manpreet Singh, Harshavardhan Thippareddi
Shiga Toxin-Producing Escherichia Coli In Meat: A Preliminary Simulation Study On Detection Capabilities For Three Sampling Methods, Julie Couton, David Marx, John Luchaansky, Randall Phebus, Anna Porto-Fett, Nicholas Sevart, Manpreet Singh, Harshavardhan Thippareddi
Conference on Applied Statistics in Agriculture
Contamination by Shiga Toxin-producing Escherichia coli (STEC) is a continuing concern for meat production facility management throughout the United States. Several methods have been used to detect STEC during meat processing, however the excessive experimental cost of determining the optimal method is rarely feasible. The objective of this preliminary simulation study is to determine which sampling method (Cozzini core sampler, core drill shaving, and N-60 surface excision) will better detect STEC at varying levels of contamination present in the meat. 1000 simulated experiments were studied using a binary model for rare occurrences to find the optimal method. We found that …