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Clinical And Mechanistic Insights Into Novel Probiotic Functions And Formulations, Jordan Bisanz
Clinical And Mechanistic Insights Into Novel Probiotic Functions And Formulations, Jordan Bisanz
Electronic Thesis and Dissertation Repository
Using a combination of hypothesis and discovery based approaches, the goal of this thesis was to better describe novel probiotic functions and their mechanisms while striving to better understand the effect of formulation on Bifidobacterium animalis subsp. lactis, Lactobacillus paracasei and L. rhamnosus.
Using RNA-Seq, a bacterial metatranscriptome analysis of a commonly consumed probiotic yogurt showed that the organisms adapted to storage time and flavor additions. This led to the discovery that in addition to the probiotic health benefits, members of the L. casei group (L. rhamnosus and L. paracasei) produce volatile sulfur compounds mediated by a novel …
Detection Of Beneficial Microbiota In Mouse Colon, Aranya Linpisanl
Detection Of Beneficial Microbiota In Mouse Colon, Aranya Linpisanl
Wayne State University Theses
Lactic acid bacteria (LAB) and Bifidobacterium are the most common types of microbes used as probiotics. They are present in the human gastrointestinal tract and have a significant influence on our health and well-being. Microbiota plays an important role in host metabolism and provides a natural defense mechanism against invading pathogens. This experiment was focusing on establish a method to detect the gastrointestinal tract microbiota, either by fecal or colonic tissue DNA extraction.
The experiment comparing 2 types of DNA extraction; ZR Fecal kit and DNAzol direct. DNAzol direct was easy to use but was not suitable for long term …
Efficacy Of Ultraviolet Radiation To Control Lactic Acid Bacteria In Wine Must, Brian D. Williams
Efficacy Of Ultraviolet Radiation To Control Lactic Acid Bacteria In Wine Must, Brian D. Williams
All Master's Theses
The use of shortwave ultraviolet (UVc) radiation to control lactic acid bacteria (LAB) in wine production was studied. A simulated wine sterilizer was built using a commercially sourced ultraviolet (UV) sterilizer commonly used in aquariums and ponds. After growing cultures in test tubes, samples of five different species of LAB were introduced into white grape juice adjusted for brix and pH to match that of wine must commonly found in the Yakima Valley American Viticultural Area. The mixture was then agitated and allowed time to evenly distribute the bacteria throughout the juice. The juice was sent through the …