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Fluorescence Characterization Of The Mature Caryopsis Of Sorghum Bicolor (L.) Moench, C. F. Earp, L. W. Rooney
Fluorescence Characterization Of The Mature Caryopsis Of Sorghum Bicolor (L.) Moench, C. F. Earp, L. W. Rooney
Food Structure
Fluorescence microscopy was used to characterize the mature car yaps is of Sorghum bicolor (L.) Moench . Acid Fuchsin, a protein specific dye used in bright field microscopy, caused protein bodies and matrix in the sorghum endosperm to fluoresce. ANS (8-ani lino-1- naphthal e ne sulfonic acid) also caused the protein bodi es and matrix in the endosperm to fluoresce. Varietal differences in endosperm protein dist r ibution were evident when viewed after sta · ning with Acid Fuchsin . Nile Blue A caused fl uorescence in neutral lipids such as those in :he lipid bodies in the aleurone and …
Microstructure Of Mealy And Vitreous Wheat Endosperms (Triticum Durum L.) With Special Emphasis On Location And Polymorphic Behaviour Of Lipids, A. Al Saleh, D. Marion, D. J. Gallant
Microstructure Of Mealy And Vitreous Wheat Endosperms (Triticum Durum L.) With Special Emphasis On Location And Polymorphic Behaviour Of Lipids, A. Al Saleh, D. Marion, D. J. Gallant
Food Structure
Dark inclusions observed in osmiophilic zones, already described in mature wheat endosperm using transmission electron microscopy, were confirmed by freeze-fracture electron microscopy to be lipids. The polar lipids (glycolipids, phospholipids, free fatty acides, monoglycerides) were organized in a liquid crystalline phase. The reversed hexagonal or HII phase should be the main lattice which might arise from the transition of lipids present in membranous structures as a lamellar phase. This transition was caused by dehydration occurring during maturation. It is suggested that the water-dependent lamellar hexagonal phase transitions are of considerable importance in cereal food technology.