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Transcriptome Analysis Of Bifidobacterium Longum Strains That Show A Differential Response To Hydrogen Peroxide Stress, Taylor S. Oberg, Robert E. Ward, James L. Steele, Jeff Broadbent

Nutrition, Dietetics, and Food Sciences Faculty Publications

Consumer and commercial interest in foods containing probiotic bifidobacteria is increasing. However, because bifidobacteria are anaerobic, oxidative stress can diminish cell viability during production and storage of bioactive foods. We previously found Bifidobacterium longum strain NCC2705 had signifi- cantly greater intrinsic and inducible resistance to hydrogen peroxide (H₂O₂) than strain D2957. Here, we explored the basis for these differences by examining the transcriptional responses of both strains to sub-lethal H₂O₂ exposure for 5- or 60-min. Strain NCC2705 had 288 genes that were differentially expressed after the 5-min treatment and 114 differentially expressed genes …