Life Sciences Commons^™

Effects Of Sensory Cues On Product Acceptability And Consumer Perceptions, Emotions, And Behavior, Cristhiam Gurdian Curran

LSU Doctoral Dissertations

Visual and cognitive cues can affect overall liking (OL) and consumer perceptions, emotions, and behavior. The first study explored the effect of product color difference on the liking, perception, and purchase intent (PI) of cheese-flavored-tortilla chips (CFTC) formulations (A and B) on serving plates (plastic, foam, and paper). Color differences between formulations influenced crunchiness and saltiness liking and perception, which together with overall flavor liking and formulation, mainly determined CFTC OL. Although having similar fracturability (N) and sodium content, formulation A had higher crunchiness and saltiness likings. PI was influenced by crunchiness, saltiness, and OF liking with 37, 49, and …

Sodium Propionate And Sodium Butyrate Promote Fatty Acid Oxidation In Hepg2 Cells Under Oxidative Stress, Kristina Jazmin Cook

LSU Doctoral Dissertations

Non-alcoholic fatty liver disease (NAFLD) is a spectrum of disturbances that includes simple steatosis, non-alcoholic steatohepatitis (NASH), fibrosis, cirrhosis, and liver cancer. NAFLD affects individuals that consume the typical Western diet consisting of high levels of fats and carbohydrates. The increase in circulating free fatty acids, palmitate and oleate, or lipopolysaccharides (LPS), induce oxidative stress and pro-inflammatory cytokine production in the liver, which all contribute to NAFLD progression. In this study, we are evaluating the mRNA expression of genes associated with fatty acid oxidation (FAO) and the protein expression of pro-inflammatory cytokines related to NAFLD using the HepG2 human liver …

Prevalence And Control Of Salmonella Spp. And Sanitary Indicator Microorganisms In Wild Caught And Farm Raised Catfish (Ictalurus Punctatus), Bilan Costley Jessie

LSU Doctoral Dissertations

Catfish inspections have transitioned from the U.S. Food and Drug Administration (FDA) to the United States Department of Agriculture (USDA). The USDA Food Safety and Inspection Service (FSIS) and Food Emergency Response Network (FERN) consider it important to assess the food safety risk associated with consuming catfish in the United States. Surveillance of farm-raised and wild-caught catfish for pathogens is relevant as a public safety protocol. The purpose of this study was to detect the prevalence of Salmonella spp., identify and examine isolates for antibiotic resistance to clinical treatments, evaluate the effectiveness of antimicrobial chemical treatments against Salmonella enterica, and …

Occurrence Of Aflatoxin And Zearalenone In Concentrate Feeds And Milk Contamination With Aflatoxin M1 And Bacterial Pathogens In Malawi, Chunala Alexico Njombwa

LSU Doctoral Dissertations

Multiple studies were conducted in Malawi to: 1) evaluate knowledge and perception of dairy farmers towards molds, mycotoxins and associated adverse effects; 2) evaluate dairy farmers’ knowledge and perception on milk contamination, proper milk handling practices and adverse health effects associated with raw milk consumption; 3) determine levels of aflatoxins and zearalenone (ZEN) in concentrate feedstuffs; 4) assess prevalence and levels of aflatoxin M₁(AFM₁) in raw milk, dietary exposure and estimate HCC risks to children and adults; 5) assess Salmonella and E. colicontamination in raw and processed milk from selected small scale farms and major …

Sodium Reduction Through Salt Substitute Mixtures, Visual Cues, And Salt Level Statements, And Its Effects On Consumer Perception: A Case Of Barbecue Sauce, Jose Ramon Alonso Marenco

LSU Doctoral Dissertations

Cardiovascular diseases are the leading causes of death in the USA and associated with excessive sodium (Na) intake. Thus, reducing the Na intake is vital to alleviate health concerns. One serving of the average Barbecue sauce (BBQs) may contribute around 15%-20% of the daily Na allowance (2,300 mg). No research has evaluated salt substitutes or visual cues on salty taste perception of BBQs, thus it was the aim of this research. Consumer studies followed a Balanced Incomplete Block (BIB) designs. For Study I, BBQs prototypes followed a 3-components (NaCl : KCl : Glycine) mixture design, with 38% - 87% less …

Antimicrobial Properties Of Different Chitosans Against Gram-Negative And Gram-Positive Foodborne Pathogens In Food Products, Nancy Katherine Rubio Zapata

LSU Doctoral Dissertations

Chitosan is a polymer derived of the deacetylation of chitin that is one of the most abundant material in nature. Chitosan solutions applied as edible coatings, and dipping solutions have shown positive results in the extension of shelf life on seafood and meat products. The purpose of this study was to evaluate the antimicrobial activities of a newly invented high molecular weight water-soluble (HMWWS) chitosan against selected Gram-negative (Salmonella Typhimurium, Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae) and Gram-positive (Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus) foodborne pathogens (initial inoculation of 6.5 Log). Chitosans with …

Improving The Food Safety Of Louisiana Strawberry Industry, Jose L. Brandao Delgado

LSU Doctoral Dissertations

Escherichia coli O157:H7, Salmonella sp., and Listeria monocytogenes have been linked to foodborne outbreaks in produce. The most recent outbreaks in produce have been associated with irrigation water due to infiltration of well water or water run-off from contaminated sources. The Food Safety Modernization Act (FSMA) requires all irrigation water to be safe for use on produce, as a strategy to reduce foodborne illnesses. A surfactant modified zeolite (SMZ) filtration system could provide produce farmers with a sustainable low-cost system for high-quality and safe irrigation water. The objective of this study was to evaluate the effectiveness of hexadecyltrimethylammonium …

Sensory Analysis For Determining The Influence Of Visual Stimuli Upon Eating Experiences, Pitchayapat Chonpracha

LSU Doctoral Dissertations

Consumers are currently more conscious about salt and sugar intake than they were in the past. Visual cues have been shown to impact consumers’ cognitive and their taste perceptions. Current research information about visual cues enhancing human’s tastes expectation of saltiness and sweetness is limited. The sophisticated sensory analysis for products containing a reduced amount of salt and sugar is undoubtedly time–consuming and costly if the sensory experiment is designed with many samples. Therefore, the following three studies were achieved: I) an examination of how intrinsic and extrinsic visual cues affect the overall consumer eating experiences; II) a comparison of …

Effect Of Environmental Factors And Residual Sanitizers On Survival And Attachment Of Indicator Organism And Human Pathogens On Fresh Produce, Vijay Singh Chhetri

LSU Doctoral Dissertations

A comprehensive understanding of the behavior of microbial contaminants on produce surfaces in agricultural farms and during post-harvest activities is essential for the development of produce safety risk management strategies. Our studies investigated the survival and the attachment of E. coli on watermelon surfaces in agricultural settings with different levels of vegetation, and the effect of residual sanitizers on the survival and the attachment of E. coli O157: H7 and L. monocytogenes on spinach leaves. The attachment strength (S_R) of the E. coli cells on watermelon surfaces significantly increased (P<0.05) from 0.04 to 0.99 in the first 24 h, which was primarily due to the decrease in loosely attached population, given that the population of strongly attached cells was constant. The daily die-off rate of E. coli ranged from -0.12 …

Blueberry Pectin-Anthocyanin Binding Kinetics And Stability Under In Vitro Digestion, Jee Won Koh

LSU Doctoral Dissertations

Blueberry pectins were sequentially extracted from highbush blueberry powder and precipitated in alcohol to obtain water soluble fraction (WSF) and chelator soluble fraction (CSF). WSF was high methoxyl pectin (degree of esterification: 53), rich in in neutral sugars (40 mol%), and had less negative charge (-21 mV) whereas CSF was low methoxyl pectin (degree of esterification: 21), rich in uronic acids (92 mol%), and had lower negative charge ( -26 mV).

The stability of blueberry pectin-anthocyanin complex was investigated under simulated in vitro digestion. Binding of blueberry pectins with three anthocyanin standards (malvidin-3-glucoside; M3G, cyanidin-3-glucoside; C3G, and delphinidin-3-glucoside; D3G) and …

Effects Of Red Raspberry Polyphenols And Metabolites On Biomarkers Of Inflammation And Insulin Resistance In Prediabetes And Type 2 Diabetes, Reynaldo Moreno Ucles

LSU Doctoral Dissertations

Berry fruits are rich sources of polyphenolic compounds (PCs), which can promote health benefits. Anthocyanin concentrations of red raspberry (RR)(Rubus idaeus) extracts were determined to be a total of 887.6 ± 262.8 μg/g cyanidin-3-sophoroside (C3S) equivalents with C3S being the most prevalent. Ellagitannin analysis by MALDI-TOF indicated sanguiin H-6 and lambertianin C were the major ellagitannins present in RR. To explore the efficacy of RR in modulating diabetes, seven type 2 diabetes mellitus (T2DM) and two pre-diabetic patients were given drinks delivering one RR serving (123 g) per day in a smoothie for two weeks. Blood samples were …

Development, Acceptability, And Emotional Responses Of Low-Sodium Roasted Peanuts And Flavored Spreads, Kairy Dharali Pujols Martinez

LSU Doctoral Dissertations

The objective of this research was to evaluate the feasibility of developing acceptable low-sodium products by using a salt-replacement technique, an oil-in-water emulsion system, and health benefit information to improve consumers’ awareness of low-sodium diets. Three experiments were conducted: (I) Sensory characteristics and optimization of low-sodium roasted peanuts by substitution of NaCl with KCl, and addition of glycine (Gly) as a bitterness blocker; (II) Rejection threshold (RjT) of KCl added to low-sodium roasted peanuts using a 2AC-test; (III) Development of acceptable low-sodium and sodium-free spreads by flavor modification and their use on turkey salad sandwiches. In study I, results showed …

Interaction Of Cocoa Powder With Intestinal Microbiota, Zuyin Li

LSU Doctoral Dissertations

Cocoa powder contains polyphenolic compounds, which can provide potential health benefits to humans. The human gastroinestinal tract hosts diverse microorganisms which are called the human gut microbiota. These microbes can utilize carbohydrates through fermentation resulting in the production of short chain fatty acids (SCFAs), which cause a reduction of pH in gastroinestinal tract. The cocoa processing can change the composition of the carbohydrates, which can affect the fermentation patterns.

In this study we established an in vitro model system which simulated gastric digestion and colon fermentation. Five different processed cocoa samples (alkalized cocoa samples: D-11-B and D-11-S; non-alkalized cocoa samples: …

Statistical And Technical Methodologies For Duplicated Multiple-Samples Preference And Attribute Intensity Sensory Ranking Test, Kennet Mariano Carabante Ordonez

LSU Doctoral Dissertations

Ranking tests are important preference and attribute difference tools for sensory evaluation. Replicated testing is used widely to reduce the number of panelists required in other sensory methods such as discrimination. The information regarding replications sensory ranking is limited. This research evaluated important statistical and technical aspects for the development of the foundation for duplicated sensory ranking tests. Three studies were accomplished: 1) A study of nonparametric analyses on real preference ranked data; 2) a sensitivity study of two samples serving protocols for duplicated visual ranking, and 3) protocols comparison in taste. In study 1, 125 panelists ranked in duplicates …

Discovering Potential Protein, Carbohydrate, And Lipid Based Food Ingredients In A Co-Culture Of Microalgae, Chelsea Tyus

LSU Doctoral Dissertations

Louisiana Native Co-Culture of Microalgae (Chlorella Vulgaris L. and Cyanobacteria Leptolyngbya sp.) (CCA) was studied. CCA is a viable coculture for further investigation as a food component. This research characterized the proteins, carbohydrates, and lipids present in CCA. Algae cultivation parameters were controlled and analyzed. Treatment (Trt) 1 was CCA grown in cultures exposed to average scalar irradiance (ASI) of 1041 ± 269.18 μmol m-2 s-1 PAR and trt 2 was CCA grown in cultures exposed to ASI of 430 ± 96.03 μmol m-2 s-1 PAR. The trt (irradiance exposure) had the desired response on CCA species ratio Trt …