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Food Science

Department of Food Science and Technology: Faculty Publications

STEC

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Full-Text Articles in Life Sciences

Use Of Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba) To Mitigate Salmonella Enterica, Shiga Toxin-Producing Escherichia Coli (Stec) And Aspergillus Flavus In Pet Food Kibbles, Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich Feb 2023

Use Of Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba) To Mitigate Salmonella Enterica, Shiga Toxin-Producing Escherichia Coli (Stec) And Aspergillus Flavus In Pet Food Kibbles, Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich

Department of Food Science and Technology: Faculty Publications

Post-processing operations of extruded pet food kibbles involve coating the product with fats and flavorings. These processes increase the risk for cross-contamination with food-borne pathogens such as Salmonella and Shiga toxin-producing Escherichia coli (STEC), and mycotoxin-producing molds such as Aspergillus spp. after the thermal kill step. In this study, the antimicrobial effects of two types of organic acid mixtures containing 2-hydroxy-4-(methylthio) butanoic acid (HMTBa), Activate DA™ and Activate US WD-MAX™, against Salmonella enterica, STEC and Aspergillus flavus when used as a coating on pet food kibbles were evaluated. Using canola oil and dry dog digest as fat and flavor …


A Review Of Shiga-Toxin Producing Escherichia Coli (Stec) Contamination In The Raw Pork Production Chain, Manirul Haque, Joseph M. Bosilevac, Byron D. Chaves Jul 2022

A Review Of Shiga-Toxin Producing Escherichia Coli (Stec) Contamination In The Raw Pork Production Chain, Manirul Haque, Joseph M. Bosilevac, Byron D. Chaves

Department of Food Science and Technology: Faculty Publications

Epidemiological evidence of Shiga toxin-producing Escherichia coli (STEC) infections associated with the consumption of contaminated pork highlight the need for increased awareness of STEC as an emerging pathogen in the pork supply chain. The objective of this review is to contribute to our understanding of raw pork products as potential carriers of STEC into the food supply. We summarize and critically analyze primary literature reporting the prevalence of STEC in the raw pork production chain. The reported prevalence rate of stx-positive E. coli isolates in live swine, slaughtered swine, and retail pork samples around the world ranged from 4.4 …


Revisiting The Stec Testing Approach: Using Espk And Espv To Make Enterohemorrhagic Escherichia Coli (Ehec) Detection More Reliable In Beef, Sabine Delannoy, Byron D. Chaves, Sarah A. Ison, Hattie E. Webb, Lothar Beutin, Jose Delaval, Isabelle Billet, Patrick Fach Jan 2016

Revisiting The Stec Testing Approach: Using Espk And Espv To Make Enterohemorrhagic Escherichia Coli (Ehec) Detection More Reliable In Beef, Sabine Delannoy, Byron D. Chaves, Sarah A. Ison, Hattie E. Webb, Lothar Beutin, Jose Delaval, Isabelle Billet, Patrick Fach

Department of Food Science and Technology: Faculty Publications

Current methods for screening Enterohemorrhagic Escherichia coli (EHEC) O157 and non-O157 in beef enrichments typically rely on the molecular detection of stx, eae, and serogroup-specific wzx or wzy gene fragments. As these genetic markers can also be found in some non-EHEC strains, a number of “false positive” results are obtained. Here, we explore the suitability of five novel molecular markers, espK, espV, ureD, Z2098, and CRISPRO26:H11 as candidates for a more accurate screening of EHEC strains of greater clinical significance in industrialized countries. Of the 1739 beef enrichments tested, 180 were positive for both stx and eae genes. Ninety (50%) …