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Quantitative Real-Time Polymerase Chain Reaction For Detecting Mycoplasma Hyosynoviae And Mycoplasma Hyorhinis In Pen-Based Oral, Tonsillar, And Nasal Fluids, João Carlos Gomes Neto, Leslie Bower, Barbara Z. Erickson, Chong Wang, Matthew Raymond, Erin L. Strait
Quantitative Real-Time Polymerase Chain Reaction For Detecting Mycoplasma Hyosynoviae And Mycoplasma Hyorhinis In Pen-Based Oral, Tonsillar, And Nasal Fluids, João Carlos Gomes Neto, Leslie Bower, Barbara Z. Erickson, Chong Wang, Matthew Raymond, Erin L. Strait
Department of Food Science and Technology: Faculty Publications
Mycoplasma (M.) hyorhinis and M. hyosynoviae are pathogens known to cause disease in pigs post-weaning. Due to their fastidious nature, there is increased need for culture-independent diagnostic platforms to detect these microorganisms. Therefore, this study was performed to develop and optimize quantitative real-time PCR (qPCR) assays to rapidly detect M. hyorhinis and M. hyosynoviae in pen-based oral fluids as well as nasal and tonsillar fluids as proxies for samples used in swine herd surveillance. Two methods of genomic DNA extraction, automated versus manual, were used to compare diagnostic test performance. A wean-to-finish longitudinal study was also carried out to demonstrate …