Life Sciences Commons^™

Analyzing Cellular Internalization Of Nanoparticles And Bacteria By Multi-Spectral Imaging Flow Cytometry, Yashdeep Phanse, Amanda E. Ramer-Tait, Sherree L. Friend, Brenda Carrillo-Conde, Paul Lueth, Carrie J. Oster, Gregory J. Phillips, Balaji Narasimhan, Michael J. Wannemuehler, Bryan H. Bellaire

Department of Food Science and Technology: Faculty Publications

Nanoparticulate systems have emerged as valuable tools in vaccine delivery through their ability to efficiently deliver cargo, including proteins, to antigen presenting cells^1-5. Internalization of nanoparticles (NP) by antigen presenting cells is a critical step in generating an effective immune response to the encapsulated antigen. To determine how changes in nanoparticle formulation impact function, we sought to develop a high throughput, quantitative experimental protocol that was compatible with detecting internalized nanoparticles as well as bacteria. To date, two independent techniques, microscopy and flow cytometry, have been the methods used to study the phagocytosis of nanoparticles. The high throughput …

Harvesting Murine Alveolar Macrophages And Evaluating Cellular Activation Induced By Polyanhydride Nanoparticles, Ana V. Chavez-Santoscoy, Lucas M. Huntimer, Amanda Ramer-Tait, Michael Wannemueler, Balaji Narasimhan

Department of Food Science and Technology: Faculty Publications

Biodegradable nanoparticles have emerged as a versatile platform for the design and implementation of new intranasal vaccines against respiratory infectious diseases. Specifically, polyanhydride nanoparticles composed of the aliphatic sebacic acid (SA), the aromatic 1,6-bis(p -carboxyphenoxy)hexane (CPH), or the amphiphilic 1,8-bis(p-carboxyphenoxy)-3,6-dioxaoctane (CPTEG) display unique bulk and surface erosion kinetics^1,2 and can be exploited to slowly release functional biomolecules (e.g., protein antigens, immunoglobulins, etc.) in vivo^3,4,5. These nanoparticles also possess intrinsic adjuvant activity, making them an excellent choice for a vaccine delivery platform^6,7,8.

In order to elucidate the mechanisms governing the activation of innate …