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Articles 1 - 16 of 16
Full-Text Articles in Life Sciences
Antibiotic Sensitivity Testing Of Foodborne Bacteria Using Surface-Enhanced Raman Spectroscopy, Joshua Gukowsky
Antibiotic Sensitivity Testing Of Foodborne Bacteria Using Surface-Enhanced Raman Spectroscopy, Joshua Gukowsky
Doctoral Dissertations
The spread of antibiotic resistant bacteria around the world has become a major public health issue, and it is essential that effective detection methods exist for identifying these organisms and preventing them from spreading throughout our food systems and into the environment. The goal of this research is to develop a novel analytical procedure that is capable of easily identifying antibiotic resistance in bacterial samples, and also provides more information about the biochemical characteristics of the bacteria and their responses to antibiotic exposure. Surface-enhanced Raman Spectroscopy (SERS), an analytical technique that uses light scattering to produce a spectrum based on …
Factors Affecting Sensory Acceptance Of Thickened Liquids Used In Dysphagia Management, Allison N. Cox
Factors Affecting Sensory Acceptance Of Thickened Liquids Used In Dysphagia Management, Allison N. Cox
Masters Theses
Between 4-16% of adults in the United States have experienced difficulty swallowing at some point during their lives. Difficulty swallowing, or clinically referred to as dysphagia, poses increased concern when drinking beverages. While no treatment is currently available, it is often recommended that liquids be thickened to improve the safety of swallowing and prevent liquids from being aspirated in the lungs. However, thickened liquids are poorly accepted by individuals with dysphagia. Taste and flavor suppression has been shown in various thickened liquid matrices, but the mechanisms for understanding these changes in perception are quite complex. Additionally, literature focused on dysphagic …
Antioxidant Synergism Between Α-Tocopherol And A High Phosphatidylserine Modified Lecithin, Harshika Arora
Antioxidant Synergism Between Α-Tocopherol And A High Phosphatidylserine Modified Lecithin, Harshika Arora
Masters Theses
Phospholipids, such as phosphatidylserine (PS) have been shown to work synergistically with tocopherols to extend the shelf life of oil-in-water emulsions. However, the high cost of PS prevents it from being used as a food additive. This work investigated the potential use of a high PS enzyme-modified lecithin to be used along with α-tocopherol to extend the lag phase of oil-in-water emulsions stabilized using Tween 20. Phospholipase D from Streptomyces sp. and L-serine were used to modify lecithin to increase PS concentration. Enzyme activity was optimized as a function of pH and temperature using a high PC soybean lecithin. The …
Antioxidant Combination Of High Phosphatidylserine (Ps) Lecithin With Mixed Tocopherol In Soybean Oil-In-Water Emulsion: Effect Of Ph And Salt, Princy Agnihotri
Antioxidant Combination Of High Phosphatidylserine (Ps) Lecithin With Mixed Tocopherol In Soybean Oil-In-Water Emulsion: Effect Of Ph And Salt, Princy Agnihotri
Masters Theses
Lipid oxidation is one of the major challenges faced by the food industry as it contributes to the loss of nutritional quality and loss of flavor in food products. Studies have shown that naturally occurring phospholipids like phosphatidylserine (PS) and phosphatidylethanolamine (PE) can regenerate oxidized tocopherols and help delay the lipid oxidation in bulk oils and oil-in-water emulsions. Since consumers desire simpler and cleaner labels, without chemically synthesized antioxidants, this research is of great interest. The combination of PS and PE with tocopherols has already been studied. However, PS was a better antioxidant in combination with tocopherols in the oil-in-water …
Fat Lowering Effects Of Fisetin In Caenorhabditis Elegans, Nikolas J. Rodriguez
Fat Lowering Effects Of Fisetin In Caenorhabditis Elegans, Nikolas J. Rodriguez
Masters Theses
Fisetin, a flavanol with anti-inflammatory, anti-cancer, and anti-aging properties, has shown promise for reducing fat accumulation in tissue culture and animal models. This plant sourced compound has limited studies supporting its effects on fat accumulation. Therefore, this study was completed to determine fisetin’s role in fat reduction along with its mechanism of action using Caenorhabditis elegans. C. elegans is a small roundworm with roughly 65% of its genes being conserved in humans related to disease. In this study, 100 and 200 µM fisetin has shown to reduce fat accumulation in wild-type worms. Body size, locomotion, and pumping rate were …
Improvement Of Solubility, Stability, And Bioaccessibility Of Curcumin Using Colloidal Delivery Systems, Bingjing Zheng
Improvement Of Solubility, Stability, And Bioaccessibility Of Curcumin Using Colloidal Delivery Systems, Bingjing Zheng
Doctoral Dissertations
Curcumin is a yellow-orange crystalline substance found in certain foods (turmeric) that is claimed to exhibit a broad range of biological activities. Its application as a nutraceutical in functional foods and beverages is often limited by its relatively low solubility in aqueous media, its chemical instability, and its low bioavailability. Recent research suggests that colloidal delivery systems can overcome these hurdles and improve the efficacy and commercial value of curcumin in the food, supplement, and pharmaceutical fields. The purpose of this research was to develop colloidal delivery systems to improve the application of curcumin as a nutraceutical in foods. First, …
Investigation On The Factors Impacting The Bioaccessibility Of Oil-Soluble Vitamins: Food Matrix Effects, Yunbing Tan
Investigation On The Factors Impacting The Bioaccessibility Of Oil-Soluble Vitamins: Food Matrix Effects, Yunbing Tan
Doctoral Dissertations
Food, nutrition, and pharmaceutical scientists are trying to elucidate the major factors impacting the bioavailability of macronutrients (e.g., lipids), micronutrients (e.g., vitamins), and nutraceuticals (e.g., carotenoids) so as to improve their efficacy. Currently, however, there is a limited understanding of how food matrix effects impact nutrient digestion and bioavailability. In this thesis, the impact of food matrix effects was elucidated by systematically examining the influence of several important factors (including oil type, oil concentration, droplet size, emulsifier type, and nutrient type) on lipid digestion and the bioaccessibility of hydrophobic bioactive agents. Oil-in-water emulsions were used as model …
Formation, Characterization, And Utilization Of Multilayer Nanoemulsions In Foods, Jorge L. Muriel Mundo
Formation, Characterization, And Utilization Of Multilayer Nanoemulsions In Foods, Jorge L. Muriel Mundo
Doctoral Dissertations
Multilayer coatings have been proposed as a promising nanotechnology for improving the performance of emulsion-based products in numerous research fields. In foods, these multilayer coatings can be used to improve the encapsulation and protection of bioactive ingredients in delivery systems during storage and passage through the gastrointestinal tract (1, 2). Therefore, there is strong interest in understanding the formation, properties, and performance of these novel coatings. Multilayer coatings are formed by layer-by-layer electrostatic deposition of oppositely charged biopolymers, such as proteins and polysaccharides. A better understanding of the formation and properties of biopolymer multilayer coatings could lead to …
Interactions Between Titanium Dioxide Nanoparticles (Nps) And Mucin, . Boimin
Interactions Between Titanium Dioxide Nanoparticles (Nps) And Mucin, . Boimin
Doctoral Dissertations
Titanium dioxide nanoparticles (TiO2 NPs) are widely used in many food, consumer, and industrial products. However, little is known about the overall effects of TiO2 NPs on the environment or human health. In order to elucidate the fate, transformation, transport, and toxicological impact of TiO2 NPs, a better understanding is needed of how the physicochemical properties of TiO2 NPs (e.g. size, charge, curvature, hydrophobicity, and surface functionality) interact with their microenvironments (e.g. pH, temperature, bile acids, microbiome, enzymes, surface-active components, and biopolymers). Living organisms including humans have a natural mechanism to protect themselves from physical, biological, …
Improving Health Targeted Food Quality Of Blackberry: Pear Fruit Synergy Using Lactic Acid Bacterial Fermentation, Nicholas Pucel, Dipayan Sarkar, Ronald G. Labbe, Chartchai Khanongnuch, Kalidas Shetty
Improving Health Targeted Food Quality Of Blackberry: Pear Fruit Synergy Using Lactic Acid Bacterial Fermentation, Nicholas Pucel, Dipayan Sarkar, Ronald G. Labbe, Chartchai Khanongnuch, Kalidas Shetty
Food Science Department Faculty Publication Series
Blackberry and pear are rich in human health protective phenolic bioactives with high antioxidant activity. These fruits are relevant dietary targets to counter chronic oxidative stress-linked diseases, such as type 2 diabetes (T2D). Due to high perishability, the human health relevant bioactive qualities of such fruits deteriorate during postharvest storage and processing. By improving stability and bioavailability of nutritionally relevant phenolic bioactives during post-harvest stages, effective integration of blackberry and pear as dietary support strategies can be targeted for T2D benefits. Solutions to bioactive quality loss of fruits can be achieved by advancing bioprocessing strategy integrating compatible fruit synergy and …
Use Of Micellar Delivery Systems To Enhance Curcumin’S Stability And Microbial Photoinactivation Capacity, Victor Ryu, Silvette Ruiz-Ramirez, Piyanan Chuesiang, Lynne A. Mclandsborough, David Julian Mcclements, Maria G. Corradini
Use Of Micellar Delivery Systems To Enhance Curcumin’S Stability And Microbial Photoinactivation Capacity, Victor Ryu, Silvette Ruiz-Ramirez, Piyanan Chuesiang, Lynne A. Mclandsborough, David Julian Mcclements, Maria G. Corradini
Food Science Department Faculty Publication Series
Microbial photoinactivation using ultraviolet (UV) or visible light can be enhanced by photosensitizers. This study assessed the efficacy of encapsulating a food-grade photosensitizer (curcumin) in surfactant micelles on its water dispersibility, chemical stability, and antimicrobial activity. Stock curcumin-surfactant solutions were prepared with Surfynol 465 (S465) or Tween 80 (T80) (5 mM sodium citrate buffer). The antimicrobial activity of curcumin-loaded surfactant solutions was determined by monitoring the inactivation of Escherichia coli O157: H7 and Listeria innocua after 5-min irradiation with UV-A light (λ = 365 nm). The solutions mixed with the bacterial suspensions contained 1 µM curcumin and each surfactant below, …
Effect Of Encapsulated Edible Halophyte With Different Biopolymers On The Inhibition Of Sodium Absorption In Mouse, Eun Young Jung, Seung Yun Lee, Da Young Lee, On You Kim, Yeonhwa Park, Sun Jin Hur
Effect Of Encapsulated Edible Halophyte With Different Biopolymers On The Inhibition Of Sodium Absorption In Mouse, Eun Young Jung, Seung Yun Lee, Da Young Lee, On You Kim, Yeonhwa Park, Sun Jin Hur
Food Science Department Faculty Publication Series
The purpose of this study was to investigate the effects of edible halophyte Salicornia herbacea encapsulated with biopolymers on inhibition of sodium absorption in mouse. Salicornia herbacea encapsulated with four biopolymers (pectin, chitosan, cellulose and dextrin) were fed to mice for 48 hr, and inhibiting sodium absorption was measured. In primary in vitro condition, fresh Salicornia herbacea encapsulated with 1% cellulose had 40% binding rate. Juice residue Salicornia herbacea encapsulated with 1% chitosan had the highest sodium binding rate by 50%. In mouse model, fresh, juice, and juice residue of Salicornia herbacea encapsulated with 4% chitosan had the highest sodium …
Infogest Inter-Laboratory Recommendations For Assaying Gastric And Pancreatic Lipases Activities Prior To In Vitro Digestion Studies, Myriam M.L. Grundy, Evan Abrahamse, Annette Almgren, David J. Mcclements, Marie Alminger, Ana Andres, Renata M.C. Ariëns, Shanna Bastiaan-Net, Claire Bourlieu-Lacanal, André Brodkorb
Infogest Inter-Laboratory Recommendations For Assaying Gastric And Pancreatic Lipases Activities Prior To In Vitro Digestion Studies, Myriam M.L. Grundy, Evan Abrahamse, Annette Almgren, David J. Mcclements, Marie Alminger, Ana Andres, Renata M.C. Ariëns, Shanna Bastiaan-Net, Claire Bourlieu-Lacanal, André Brodkorb
Food Science Department Faculty Publication Series
In vitro digestion studies often use animal digestive enzyme extracts as substitutes of human gastric and pancreatic secretions. Pancreatin from porcine origin is thus commonly used to provide relevant pancreatic enzymes such as proteases, amylase and lipase. Rabbit gastric extracts (RGE) have been recently introduced to provide gastric lipase in addition to pepsin. Before preparing simulated gastric and pancreatic extracts with targeted enzyme activities as described in in vitro digestion protocols, it is important to determine the activities of enzyme preparations using validated methods. The purpose of this inter-laboratory study within the INFOGEST network was to test the repeatability and …
Utilization Of Polysaccharide-Based High Internal Phase Emulsion For Nutraceutical Encapsulation: Enhancement Of Carotenoid Loading Capacity And Stability, Chi Yan, Xiaolin Wu, Yi Wang, Shengfeng Peng, Jun Chen, Liqiang Zou, David Julian Mcclements, Wei Liu
Utilization Of Polysaccharide-Based High Internal Phase Emulsion For Nutraceutical Encapsulation: Enhancement Of Carotenoid Loading Capacity And Stability, Chi Yan, Xiaolin Wu, Yi Wang, Shengfeng Peng, Jun Chen, Liqiang Zou, David Julian Mcclements, Wei Liu
Food Science Department Faculty Publication Series
The main goal of the present work was to access the ability of high internal phase emulsions (HIPEs) to encapsulate fl-carotene. The carotenoid loading capacity of the HIPEs was around 20-fold higher when OSAstarch/chitosan complexes were used than when only OSA-starch was used. This impact could be mainly assigned to the capacity of the former HIPEs to trap carotenoid caystals in a stable form. The OSA-starch/chitosan complexes were shown to absorb on the oil droplets interface and form a 3D network in the aqueous phase, which helped to prevent droplet coalescence induced by fl-carotene crystal. The incorporation of fl-carotene within …
Development Of Salt- And Gastric-Resistant Whey Protein Isolate Stabilized Emulsions In The Presence Of Cinnamaldehyde And Application In Salad Dressing, Huanhuan Cui, Qihang Liu, David Julian Mcclements, Bin Li, Shilin Liu, Yan Li
Development Of Salt- And Gastric-Resistant Whey Protein Isolate Stabilized Emulsions In The Presence Of Cinnamaldehyde And Application In Salad Dressing, Huanhuan Cui, Qihang Liu, David Julian Mcclements, Bin Li, Shilin Liu, Yan Li
Food Science Department Faculty Publication Series
Protein-stabilized emulsions tend to be susceptible to droplet aggregation in the presence of high ionic strengths or when exposed to acidic gastric conditions due to a reduction of the electrostatic repulsion between the protein-coated droplets. Previously, we found that incorporating cinnamaldehyde into the oil phase improved the resistance of whey protein isolate (WPI)-stabilized emulsions against aggregation induced by NaCl, KCl and CaCl2. In the current study, we aimed to establish the impact of cinnamaldehyde on the tolerance of WPI-stabilized emulsions to high salt levels during food processing and to gastric conditions. In the absence of cinnamaldehyde, the addition of high …
Anti-Obesity Effects Of Morus Alba L. And Aronia Melanocarpa In A High-Fat Diet-Induced Obese C57bl/6j Mouse Model, Na-Yeon Kim, Shalom Sara Thomas, Dae-Il Hwang, Ji-Hye Lee, Kyung-Ah Kim, Youn-Soo Cha
Anti-Obesity Effects Of Morus Alba L. And Aronia Melanocarpa In A High-Fat Diet-Induced Obese C57bl/6j Mouse Model, Na-Yeon Kim, Shalom Sara Thomas, Dae-Il Hwang, Ji-Hye Lee, Kyung-Ah Kim, Youn-Soo Cha
Nutrition Department Faculty Publication Series
The present study investigated the synergic effect of extracts of Morus alba (MA) and Aronia melanocarpa (Michx.) (AR) against high-fat diet induced obesity. Four-week-old male C57BL/6J mice were randomly divided into five groups that were fed for 14 weeks with a normal diet (ND), high-fat diet (HD), HD with M. alba 400 mg/kg body weight (MA), HD with A. melanocarpa 400 mg/kg body weight (AR), or HD with a mixture (1:1, v/v) of M. alba and A. melanocarpa (400 mg/kg) (MA + AR). Treatment with MA, AR, and MA + AR for 14 weeks reduced high fat diet-induced weight gain …