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Full-Text Articles in Life Sciences

The Rapid Enumeration Of A Mixed Culture Of S. Cerevisiae & L. Plantarum In Beer Using Image-Based Cytometry, Kevin M. Kennedy Aug 2022

The Rapid Enumeration Of A Mixed Culture Of S. Cerevisiae & L. Plantarum In Beer Using Image-Based Cytometry, Kevin M. Kennedy

Electronic Theses and Dissertations

Mixed cultures are prevalent in the food industry. From sour beer to sourdough bread, mix culture popularity is growing. A number of microbiological mixtures are used in these unique fermenting processes to create distinctive flavor profiles of consumers’ favorite foods. Although mixed cultures seem ubiquitous, they are often not well enumerated. The aim of this thesis was to create a novel rapid image-based cytometry method to enumerate mixed cultures in beer. Imaged-based cytometry can be used to rapidly enumerate mixed microbial cultures, as opposed to traditional plate counting methods that can take days to grow and count. A novel method …


Career Readiness Of Recently Credentialed Registered Dietitian Nutritionists, Maegan Perrault Aug 2022

Career Readiness Of Recently Credentialed Registered Dietitian Nutritionists, Maegan Perrault

Electronic Theses and Dissertations

This study assessed the perceptions of career preparedness of recently credentialed Registered Dietitian Nutritionists (RDNs) (< 5 years/post credential) and their experiences in undergraduate, supervised practice, and/or graduate programs. All participants were recruited from a randomized sample from the databank of the Commission on Dietetic Registration (CDR). Participants were recently credentialed RDNs (≤5 years) from the United States (U.S). A survey was designed to evaluate the RDNs perceived ability to perform the 2017 Accreditation Standards for Nutrition and Dietetics Internship Program competencies as a working RDN. Participants were also invited to share their insights by answering open-ended questions regarding their experiences as a student in undergraduate, supervised practice, and/or graduate programs. Survey responses were analyzed overall and were grouped into six domains of practice, corresponding to the Standards of Professional Practice (SOPP) domains of the Academy of Nutrition and Dietetics. The level of education, type of undergraduate degree and type of dietetic internship were evaluated for their impact on perceived career preparedness overall and within the domains of the SOPPs. Respondents agreed that they felt prepared overall to practice as an RDN in the work setting as indicated by a mean score of 2.05 (SD=0.63). Respondents felt least prepared in the competency of “analyzing quality financial and productivity data for use in planning” M= 3.16 (SD=1.66). Conversely, respondents strongly agreed that they were able to “incorporate critical thinking in overall practice” M=1.48 (SD=.60) and “practice according to the Code of Ethics” M=1.48 (SD=.64). The domain rated strongest among respondents was Domain 2: Competence and Accountability, M=1.72 (SD = 0.56). The domain with the weakest score was Domain 6: Utilization and Management of Resources M=2.93 (SD = 1.31). There were no significant differences in perceived career preparedness overall, or by domains of practice in regard to the level of education, type of undergraduate degree, and type of dietetic internship. Knowledge gaps were identified in clinical experiences and business management. In conclusion, recently credentialed RDNs in the US who have completed ACEND® programs feel ready to practice. The type of dietetic internship, level of education, and type of undergraduate degree were not significant factors in their perceived career preparedness overall or by domain of practice. The domain of management of resources and the theme of business management were consistently the areas identified as knowledge gaps. These areas should be improved upon within dietetic programs at all levels to ensure students continue to feel prepared to work in the dynamic field of healthcare.


Quantifying Spatial Heterogeneity Of Wild Blueberries And Crop Water Stress Monitoring Using Remote Sensing Technologies, Kallol Barai Aug 2022

Quantifying Spatial Heterogeneity Of Wild Blueberries And Crop Water Stress Monitoring Using Remote Sensing Technologies, Kallol Barai

Electronic Theses and Dissertations

The wild blueberry is one of the major crops of Maine, with significant economic value and potential health benefits. Due to global climate change, drought impacts have been increasing significantly in recent years in the northeast region of the USA, causing significant economic losses in the agricultural sectors. It has been predicted to increase further in the future. Changing patterns of the elevated atmospheric temperatures, increased rainfall variabilities, and more frequent drought events have made the wild blueberry industry of Maine vulnerable, suggesting the adoption of novel approaches to mitigate the negative impacts of global climate changes. Also, wild blueberry …


Prevention Of Inflammatory Bowel Disease By Broccoli-Sourced And Microbially-Produced Bioactives, Johanna M. Holman Aug 2022

Prevention Of Inflammatory Bowel Disease By Broccoli-Sourced And Microbially-Produced Bioactives, Johanna M. Holman

Electronic Theses and Dissertations

Inflammatory Bowel Diseases (IBD) are chronic, reoccurring, and debilitating conditions characterized by inflammation in the gastrointestinal tract, some of which can lead to more systemic complications and can include autoimmune dysfunction, a change in the taxonomic and functional structure of microbial communities in the gut, and complicated burdens in a person’s daily life. Like many diseases based in chronic inflammation, research on IBD has pointed towards a multifactorial origin involving factors of the host’s lifestyle, immune system, associated microbial communities, and environmental conditions. Too often, research focuses on just one aspect of IBD or uses one model with a narrow …


Research Skills Combining Microbes And Social Equity (Avs 590-0980), Sue Ishaq, School Of Food And Agriculture May 2022

Research Skills Combining Microbes And Social Equity (Avs 590-0980), Sue Ishaq, School Of Food And Agriculture

General University of Maine Publications

To understand a microbiome, you must learn about the bustling community of microorganisms and the complex ecosystems they live in, because one cannot exist without the other. So, too, does microbiome research rely on understanding the lives and ecology of humans, because there is no aspect of human life which does not involve microbes in some way. To become better microbiome researchers, we must understand social and environmental contexts which affect humans and, in many cases, prevent them from making choices which result in beneficial microbial exposures. This symposium will focus on developing research skills to create transformative research, including …


Sustainable Postharvest Processing And Value-Addition Of Aquacultured Seaweed, Samuel Akomea-Frempong May 2022

Sustainable Postharvest Processing And Value-Addition Of Aquacultured Seaweed, Samuel Akomea-Frempong

Electronic Theses and Dissertations

The annual growth rate of harvested edible seaweed in the United States’ developing seaweed aquaculture sector leaped from 8% in 2014 to a predicted 18 – 25% from 2019 – 2025 due to increased demand. For continuous growth of the edible seaweed market, addressing challenges in food safety, perishability, processing, and product development are vital. The specific objectives of this research were to: 1) evaluate the effect of pre-freezing blanching procedures on the qualities of frozen sugar kelp, 2) evaluate the impact of blanching, freezing and fermentation on kelp quality, 3) determine the effect of rehydration temperatures on kelp quality, …


Modification Of Cellulose Nanofibrils (Cnfs) To Develop A Hydrophobic And Anti-Oxidative Food Packaging Film, Suriyaprakaash Lakshmibalasubramaniam May 2022

Modification Of Cellulose Nanofibrils (Cnfs) To Develop A Hydrophobic And Anti-Oxidative Food Packaging Film, Suriyaprakaash Lakshmibalasubramaniam

Electronic Theses and Dissertations

A majority of the polymers obtained from fossil fuels are synthetic and microorganisms do not possess the enzymatic machinery to degrade them. Therefore, these materials persist and harm our environment and our ecosystems. An alternative approach to the use of synthetic polymers would be to use biodegradable materials, materials that are already found in nature. One such material, cellulose nanofibrils (CNFs), are nano scale fibers obtained from mechanical disintegration of paper pulp. Due to their physico-chemical properties, they can form strong interconnected films with good barrier properties, with the potential to replace fossil fuel based synthetic food packaging. However, being …


How Weather Affects Total Mixed Rations And Why It Is Significant To Dairy Cattle, Juliana Baranowski May 2022

How Weather Affects Total Mixed Rations And Why It Is Significant To Dairy Cattle, Juliana Baranowski

Honors College

The objective of this study was to determine how weather affects the dry matter percentage of a TMR fed to dairy cattle. Daily feed samples were collected from the University of Maine’s J.F. Witter Teaching and Research Center and stored in a freezer until dry matters could be determined. The feed samples were later thawed out, then placed in an oven at 60oC for a minimum of three days. This allowed all the moisture to be evaporated so the DM could be calculated. The maximum, minimum and average temperatures, as well as the precipitation and humidity were obtained daily from …


Envisioning A Bold Food Waste Policy For Maine: A Mixed-Methods Study Into The Context Of Landfill Diversion Of Food Waste, Dominique Danielle Dispirito May 2022

Envisioning A Bold Food Waste Policy For Maine: A Mixed-Methods Study Into The Context Of Landfill Diversion Of Food Waste, Dominique Danielle Dispirito

Honors College

Landfilled organics waste both natural and financial resources by discarding usable materials that could bolster food security programs and composting efforts. According to the Drawdown Project, one-third of the food we produce in the United States goes to the landfill without ever reaching someone’s plate, contributing to leachate at disposal sites and accounting for more greenhouse gasses than the entire airline industry. As communities across the state struggle to support the 1 in 6 Mainers experiencing food insecurity with dwindling financial resources and limited personnel, food waste diversion provides a local solution that bolsters resilience at low cost. The absence …


The Potential Use Of Tree Leaf Silage For Livestock Nutrition, Including Willow, Drumstick, Mulberry, And Acadia Species, Jade Chin Apr 2022

The Potential Use Of Tree Leaf Silage For Livestock Nutrition, Including Willow, Drumstick, Mulberry, And Acadia Species, Jade Chin

Honors College

Leaf silage has been historically used as ruminant feed, with multiple positive attributes such as being a secure, preserved feed source that is relatively accessible. However, the digestibility, nutritive value, and potential anti-nutritive factors are not well researched or known. The goal of this review is to examine recent literature on leaf silage and its potential advantages and practical limits of being utilized as livestock feed. The scope of this study was limited to willow (Salix spp.), acacia (Acacia spp.), mulberry (Morus alba and Broussonetia papyrifera), and drumstick leaf silage (Moringa oleifera), due to …