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Research On Degenerate Primers For Detection Of Staphylococcus Aureus Enterotoxin A, B Gene, Liu Jichao, Chen Bairu, Jiang Tiemin, Chen Lijun
Research On Degenerate Primers For Detection Of Staphylococcus Aureus Enterotoxin A, B Gene, Liu Jichao, Chen Bairu, Jiang Tiemin, Chen Lijun
Food and Machinery
The degenerate primers were designed to establish a PCR assay for synchronous and fast detection of staphylococcal enterotoxin A and B. According to the sequences of SEA and SEB gene, primers were designed and used for PCR. The lengths of the amplified primers were 105 bp and 135 bp. To evaluate the specificity of PCR assay, 2 strains of SEA, SEB and 4 strains of non-SEA-B were tested by PCR. SEB was 10-fold serially diluted and amplified by PCR to verify its sensitivity. The results showed that the detection of Staphylococcus aureus enterotoxin A, B strain DNA results were positive …