Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 5 of 5
Full-Text Articles in Life Sciences
Development Of Bacteriophage Based Detection Technique For Food Safety And Environment Monitoring, Ziyuan Wang
Development Of Bacteriophage Based Detection Technique For Food Safety And Environment Monitoring, Ziyuan Wang
Doctoral Dissertations
Foodborne or waterborne pathogens which are responsible for numerous worldwide outbreaks of disease have caused serious health problems and enormous economic cost. Development of new bacterial detection technologies with high sensitivity and specificity is of great importance for food safety and public health. This has highlighted the significance of developing rapid and effective techniques for pathogen screening. Thus we aim to explore a bacteriophage mediated detection system for foodborne or waterborne bacteria monitoring. Bacteriophage as a novel recognition probe has been applied in various biosensor by immobilizing phage particles on solid sensor surface. They possess high specificity for targeted microorganism …
Bacteriophage-Based Colorimetric Detection Of Escherichia Coli In Drinking Water, Juhong Chen
Bacteriophage-Based Colorimetric Detection Of Escherichia Coli In Drinking Water, Juhong Chen
Doctoral Dissertations
One of the major safety causes in drinking water is from the bacteria contamination, especially in developing countries and resource-limited settings. Although many of these Escherichia coli (E. coli) strains in drinking water are nonpathogenic, they sever as the indicator for bacterial contamination. And, the more widely used method to detect E. coli in drinking water is to determine the activity of β-galactosidase (β-gal), which is released by E. coli. Rapid, sensitive and inexpensive detection of E. coli in drinking water can reduce the risk of food-borne bacteria infection and stop the disease widely spreading. In this …
Advanced Materials For Rapid Diagnostics In Food, Agriculture And Healthcare, Charmaine K.W. Koo
Advanced Materials For Rapid Diagnostics In Food, Agriculture And Healthcare, Charmaine K.W. Koo
Doctoral Dissertations
Paper based devices are an emerging trend for micro total analysis systems due to their rapid, sensitive and specific attributes. Lateral flow assays (LFAs), the predecessor of paper-fluidic devices, are developed for many applications which range from nucleic acid and antibody detection to commercial home pregnancy tests. With growing interest in replacing conventional detection methods, multistep assays are needed. These assays require multiple test reagents, therefore, there is a need to control fluid flow for the development of complex paper-based devices. We were able fabricate paper-fluidic platforms where we used electrowetting-on-dielectrics to create valves on paper. With this method, we …
Bacteriophage: Bioengineered Bacterial Detection And Applications, Samuel D. Alcaine
Bacteriophage: Bioengineered Bacterial Detection And Applications, Samuel D. Alcaine
Doctoral Dissertations
Bacteria are ubiquitous and vital constituents of our environment, our foods, and our bodies. A small percentage of this vast, microbial population is pathogenic to humans, but represents a significant burden on public health. There is a current public health focus on two subgroups: foodborne pathogenic bacteria and antibiotic resistance bacteria. A key challenge for public health is the rapid identification of these bacteria to prevent their consumption and to ensure proper treatment for infections. This challenge calls for the development of novel, low-cost diagnostics that combine sensitivity and accuracy with speed and ease-of-use. Bacteriophages represent rapid, readily targeted, and …
Acoustic Emission Of Lactococcus Lactis Ssp. Lactis C2 Infected With Three Bacteriophages C2, Sk1 And Ml3, Luxi Meng
Theses and Dissertations--Animal and Food Sciences
The objective of this research was to monitor the Acoustic Emission (AE) produced by Lactococcus lactis ssp. lactis C2 infected with three bacteriophages (c2, sk1 and ml3 at 90 min) using an acoustic emission-monitoring device which was designed at University of Kentucky in Lexington KY. The acoustic emission data was collected and then analyzed. These Acoustic Emission (AE) data suggested that bacteriophage ml3, sk1 and c2 can easily be distinguished by the differences in Absolute Energy (ABE), Centroid Frequency (CF) and Peak Frequency (PF) signals. The AE data suggested that bacteriophage sk1 and c2 caused greater stress on the lactis …