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Changsha University of Science and Technology
Aeromonas hydrophila; lipopolysaccharide; extract and purify; cell model
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Study On Extraction And Toxicity Of Lipopolysaccharide From Aeromonas Hydrophila, Jiang Donglei, Liu Yan, Ge Panwei, Wu Mangang, Ge Qingfeng, Fang Weiming
Study On Extraction And Toxicity Of Lipopolysaccharide From Aeromonas Hydrophila, Jiang Donglei, Liu Yan, Ge Panwei, Wu Mangang, Ge Qingfeng, Fang Weiming
Food and Machinery
To study the lipopolysaccharide (LPS) of Aeromonas hydrophila, LPS was extracted by hot phenol-water method, purified by enzymatic hydrolysis and chloroform/methanol precipitation. The yield of LPS and the contents of protein, DNA were determined. The purity of LPS was analyzed by SDS-PAGE and silver staining．Ana-1 cell model was established to evaluate the toxicity and biological activity of LPS samples. The results showed that the yield of LPS was 1.22%, and the contents of protein and nucleic acid were 1.53% and 0.79% respectively. The main bands of SDS-PAGE were clear and located at same position as the LPS standard. After LPS …