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Full-Text Articles in Life Sciences
Detection Of Mycotoxins Using Surface-Enhanced Raman Spectroscopy, Lourdes B. Martinez Rojas
Detection Of Mycotoxins Using Surface-Enhanced Raman Spectroscopy, Lourdes B. Martinez Rojas
Doctoral Dissertations
Mycotoxins are toxic metabolites produced by fungus that can be parasites or saprophytes of crops or livestock forage. Consumer demand for plant-based foods and interest in animal-based foods originating from animals fed plant-based feed has been on the rise. Therefore, monitoring mycotoxins occurring in the food supply is more critical than ever. The goal of this project is to improve surface-enhanced Raman spectroscopy’s (SERS) ability to identify and detect mycotoxins using label-free SERS substrates. Two simple approaches were designed to enhance the detection of mycotoxins produced by the Aspergillus and Penicillium genera, ochratoxin A and aflatoxin B1. Ochratoxin A was …
Enzymatic Digestion Improved Bacteria Separation From Leafy Green Vegetables, Danhui Wang
Enzymatic Digestion Improved Bacteria Separation From Leafy Green Vegetables, Danhui Wang
Masters Theses
An effective and rapid method for the separation of bacteria from food matrix remains a bottleneck for rapid bacteria detection for food safety. Bacteria can strongly attach to the food surface or internalize within the matrix which makes their isolation extremely difficult. Traditional methods of separating bacteria from foods routinely involve stomaching, blending and shaking, however these methods may not be efficient at removing all the bacteria from complex matrices. Here, we investigate the benefits of using enzyme digestion followed by immunomagnetic separation to isolate Salmonella from spinach and lettuce. Enzymatic digestion using pectinase and cellulase was able to break …
Layer-By-Layer Antimicrobial N-Halamine Polymer Coatings For Food Contact Materials, Luis J. Bastarrachea Gutierrez
Layer-By-Layer Antimicrobial N-Halamine Polymer Coatings For Food Contact Materials, Luis J. Bastarrachea Gutierrez
Doctoral Dissertations
Cross contamination during food processing represents a risk for public health and financial burden. Surface modification of food contact materials to render them antimicrobial can be effective against such risk. The objective of the present work was to develop antimicrobial coatings with the potential to be applied in a variety of food contact materials. The polymer coatings developed became antimicrobial by incorporation of a type of chlorinated compounds called N-halamines, capable of regenerating their antimicrobial activity. Two layer-by-layer (LbL) assembly surface modification procedures were followed. In the first procedure, bilayers of branched polyethyleneimine (PEI) and poly(acrylic acid) (PAA) were applied …