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Full-Text Articles in Life Sciences

Development Of Novel Pectinase And Xylanase Juice Clarification Enzymes Via A Combined Biorefinery And Immobilization Approach, Shady Hassan Dec 2020

Development Of Novel Pectinase And Xylanase Juice Clarification Enzymes Via A Combined Biorefinery And Immobilization Approach, Shady Hassan

Doctoral

Hydrolytic enzymes, such as pectinase and xylanase, maybe harnessed for numerous industrial applications in food industry. Therefore, economic factors such as achievement of optimum yieldsandoverall production costs, in addition to biocatalyst instability,are the main obstacles tothe industrial production and exploitation of a enzymes. For example, microbially-derived enzymes are typically produced in fermenters using expensive growth media, which may account for 30 to 40% of the production cost, and such expense may be compounded further by downstream processing operations.To counter such disadvantages, the major trend in industrial utilization of enzymes in cost-sensitive processes has been to immobilize such biocatalysts on a …


Chemical Effects Of Cold Atmospheric Plasma On Food Nutrients, Juan Manuel Pérez Andrés Nov 2020

Chemical Effects Of Cold Atmospheric Plasma On Food Nutrients, Juan Manuel Pérez Andrés

Doctoral

A range of nonthermal techniques have demonstrated process efficacy in ensuring food product safety, extension of shelf-life and in general a retention of key quality attributes. However, various physical, chemical and biochemical effects of nonthermal techniques on both macro and micronutrients are evident, leading to both desirable and undesirable changes in food products. It is important to outline the effects of non-thermal techniques on food chemistry and the associated degradation mechanisms with the treatment of foods. Oxidation is one of the key mechanisms responsible for undesirable effects induced by non-thermal techniques. Degradation of key macromolecules largely depends on the processing …


Development And Diagnostics Of Novel Non-Thermal Plasmatreatment Systems, Laurence Scally Jan 2019

Development And Diagnostics Of Novel Non-Thermal Plasmatreatment Systems, Laurence Scally

Doctoral

Non-thermal plasma (NTP) has been a point of interest in many areas over the last few decades. Much research has been,and continues to beundertaken,to understand the fundamentals of plasma discharges. This is such a broad topic due to the very nature and variable dependencies that set the conditions for plasma discharge to occur. This can come in the form of electrode geometry and spacing, dielectric barrier thickness, humidity of environment, material selection for electrodes and dielectric barriers, the power supply used, and the operating gas(es) used. A lot of these influencing factors can be set and kept constant, but still …


Microbiological Control And Mechanisms Of Action Of High Voltage Atmospheric Cold Plasma, Lu Han Jan 2016

Microbiological Control And Mechanisms Of Action Of High Voltage Atmospheric Cold Plasma, Lu Han

Doctoral

Dielectric barrier discharge-atmospheric cold plasma (DBD-ACP) is a promising non-thermal technology effective against a wide range of microorganisms. These studies were performed using a custom built DBD-ACP system. The inactivation efficacy was found to be governed by a series of critical control parameters, including treatment time, mode of exposure (Direct/Indirect exposure), applied voltage, applied gas content but was also very dependent on the characteristics of treatment targets. In this study, these parameters were investigated using in-package design along with a post-treatment storage procedure to align with industrial processing times as well as mitigation of post-process contamination. A range of food …


Innovative Food Product Development Using Molecular Gastronomy: A Focus On Flavour And Sensory Evaluation, Mark Traynor Jan 2013

Innovative Food Product Development Using Molecular Gastronomy: A Focus On Flavour And Sensory Evaluation, Mark Traynor

Doctoral

The primary goal of this research was to develop novel ice cream products using the principles of molecular gastronomy. An ice cream model system (emulsion) was developed, in which the effects of ingredient levels on stability and formation were investigated and optimised using Response Surface Methodology (RSM). Two characteristic volatiles of banana (isoamyl acetate and furfuryl acetate) were added to the optimised emulsion, and their headspace emission was quantified using Solid Phase Microextraction with Gas Chromatography Mass Spectrometry. RSM was used to investigate the main and interactive effects of ingredient, salt and pH levels on the headspace emission of these …


Metabolic Profiling And Fingerprinting For The Detection And Discrimination Of Mechanical Damage In Mushrooms (Agaricus Bisporus) During Storage, Aoife O'Gorman Oct 2010

Metabolic Profiling And Fingerprinting For The Detection And Discrimination Of Mechanical Damage In Mushrooms (Agaricus Bisporus) During Storage, Aoife O'Gorman

Doctoral

Horticultural products such as mushrooms are exposed to external agents during their postharvest life, which are going to affect product quality. Loss of whiteness during storage is particularly important in the mushroom industry. Rough handling and distribution of crops, fruiting body senescence, bacterial and viral infection are among the causes of mushroom discolouration. The aim of this work was to study the use of metabolic fingerprinting and metabolic profiling tools for the detection and discrimination of mechanical damage on mushrooms. This research involved: 1. Investigating whether the chemical changes induced by mechanical damage and ageing of mushrooms could be (a) …


The Effect Of An Altered Cytokinin Metabolism On Plant Development And C Ell Aging In Crop Plants, Ian Mcevoy Jan 2004

The Effect Of An Altered Cytokinin Metabolism On Plant Development And C Ell Aging In Crop Plants, Ian Mcevoy

Doctoral

Isopentenyl transferase (IPT) is an enzyme encoded by a bacterial gene that catalyzes the rate-limiting step in cytokinin biosynthesis. Under the control of senescence-associated promoter (pSAG12), isolated from Arabidopsis thaliana, it has been shown to delay senescence in several herbaceous species. Cytokinin synthesis begins at the onset of senescence and is then stopped when senescence ceases. This auto regulatory control of the transgene ensures normal development and morphology of the transformed plant, which may be affected in plants constitutively expressing ipt. In this study, it was investigated if an altered cytokinin metabolism, as a result of transformation by pSAG12: ipt, …