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Breaking Virulent: The Coincidental Evolution Of Virulence Factors In Bacteria., Rhiannon Emmanuelle Cecil Dec 2023

Breaking Virulent: The Coincidental Evolution Of Virulence Factors In Bacteria., Rhiannon Emmanuelle Cecil

Electronic Theses and Dissertations

Understanding how innocuous organisms can evolve to be pathogenic to humans is of increasing global concern. Further, understanding how existing pathogens may evolved to be more virulent is also vital to our ability to provide healthcare to people afflicted with diseases that promote chronic bacterial infections, such as cystic fibrosis. With the rise of antibiotic resistance in both bacteria and fungi it is paramount that new therapeutics are identified. Understanding what mutations occur that result in increased virulence in microbes can potentially provide new targets for antimicrobial drugs to combat antibiotic resistance. The Coincidental Evolution Hypothesis is a fundamental hypothesis …


Genetic Effects Of Anthropogenic Disturbance On Native Charrs, Brad Erdman Aug 2023

Genetic Effects Of Anthropogenic Disturbance On Native Charrs, Brad Erdman

Electronic Theses and Dissertations

Artificial propagation has been utilized for over a century to offset anthropogenic declines of abundance for many fishes. Complex and poorly documented histories of habitat degradation and stockings have resulted in considerable uncertainty regarding whether contemporary populations are of native, hatchery, or mixed origins. This uncertainty is problematic as it precludes prioritizing the conservation of native populations that are postulated to possess local adaptations and greater evolutionary potential. Population genetics can assess the relative reproductive contributions of previous stocking events and in this dissertation I apply these methods to four empirical studies of native charr (genus Salvelinus) that have …


New Species Of Dryolestoid From The Late Cretaceous Allen Formation And Implications For South American Faunal Diversity., Brigid Erin Connelly Aug 2023

New Species Of Dryolestoid From The Late Cretaceous Allen Formation And Implications For South American Faunal Diversity., Brigid Erin Connelly

Electronic Theses and Dissertations

Dryolestoids are extinct cladotherians mammals from the Jurassic and Cretaceous periods. I describe a collection of dryolestoid specimens from the Late Cretaceous localities of Cerro Tortuga (Allen Formation), Anfiteatro 1, and Shining (both La Colonia Formation) from Patagonia, Argentina. Using comparative morphology, I identify a new species of meridiolestidan dryolestoid based on eleven specimens across both formations. The new species’ recovery from La Colonia Formation represents the first dryolestoid connection between the two approximately contemporaneous formations. The species’ morphology may represent an ecological shift within Meridiolestida from insectivory to herbivory, showing a transition in characters between the plesiomorphic sharp-toothed meridiolestidans …


Geometric Morphometric Analysis Of Modern Viperid Vertebrae Facilitates Identification Of Fossil Specimens, Lance D. Jessee Aug 2023

Geometric Morphometric Analysis Of Modern Viperid Vertebrae Facilitates Identification Of Fossil Specimens, Lance D. Jessee

Electronic Theses and Dissertations

Snake vertebrae are common in the fossil record, whereas cranial remains are generally fragile and rare. Consequently, vertebrae are the most commonly studied fossil element of snakes. However, identification of snake vertebrae can be problematic due to extensive variation. This study utilizes 2-D geometric morphometrics and canonical variates analysis to 1) reveal variation between genera and species and 2) classify vertebrae of modern and fossil eastern North American Agkistrodon and Crotalus. The results show that vertebrae of Agkistrodon and Crotalus can reliably be classified to genus and species using these methods. Based on the statistical analyses, four of the …


Comparative Energetics Of Mammalian Thermoregulatory Physiology, Ana M. Breit May 2023

Comparative Energetics Of Mammalian Thermoregulatory Physiology, Ana M. Breit

Electronic Theses and Dissertations

Endothermy allows species to decouple body temperature from environmental
temperatures but does not equate to endothermic species maintaining those constant temperatures. Instead, heterothermy fluctuating body temperatures, both in and outside of torpor is common and allows endotherms to expand the limits of thermoneutrality. Thermolability is likely to be more common in the tropics and subtropics, where species live within or above their thermoneutral zone. My dissertation research focused on the heterothermic-homeothermic continuum, specifically quantifying where on the continuum different species fall at certain times and why those species have evolved to be at those points. I quantified the thermal profile …


Vibroacoustic Response Of The Tympanic Membrane To Hyoid-Borne Sound Generated During Echolocation In Bats, Chelsie Snipes May 2023

Vibroacoustic Response Of The Tympanic Membrane To Hyoid-Borne Sound Generated During Echolocation In Bats, Chelsie Snipes

Electronic Theses and Dissertations

The hyoid apparatus in laryngeally echolocating bats forms a mechanical connection between the larynx and auditory bullae and has been hypothesized to transfer the outgoing echolocation call to the middle ear during echolocation call emission. We used µCT data to build models of the hyoid apparatus and middle ear from six species of bats and used finite element modeling (FEM) to measure the vibroacoustic response of the tympanic membrane due to hyoid-borne sound generated during echolocation. We found that hyoid-borne sound in all six species stimulated the eardrum within a range likely heard by bats. Although there were minor differences …


A Comparison Of Microsatellites And Single Nucleotide Polymorphisms For The Assessment Of Population Structure In The Amblyomma Maculatum Koch, 1844 Species Complex, Henrey A. Deese Jan 2023

A Comparison Of Microsatellites And Single Nucleotide Polymorphisms For The Assessment Of Population Structure In The Amblyomma Maculatum Koch, 1844 Species Complex, Henrey A. Deese

Electronic Theses and Dissertations

The Gulf Coast tick, Amblyomma maculatum Koch, 1844 is widespread throughout North and Central America and is the primary vector for the emerging pathogen Rickettsia parkeri. In the U.S., the population in the Southwest (A. maculatum morphotype III) is morphologically different from the population east of the Rocky Mountains (A. maculatum morphotype II). This research tests the hypothesis that A. maculatum morphotype II and morphotype III represent distinct species through the analysis and comparison of 6 microsatellite loci and suite of 135,221 Single nucleotide polymorphisms (SNPs) generated from ddRADseq. Population genetic analysis of both microsatellites and SNPs …


The Organizational Evolution Of Small Food Buying Clubs, Taylor Lange May 2022

The Organizational Evolution Of Small Food Buying Clubs, Taylor Lange

Electronic Theses and Dissertations

Organizations are pervasive in modern society and the factors of their evolution are the subject of considerable scholarship. Most literature on organizational evolution focuses on the role of leaders and entrepreneurs, specifically their decision making interacts with market forces. However, the behavior and interactions of regular organization members, such as nonmanagerial employees or club members, is surprisingly overlooked. Specifically, examinations of social dilemmas between co-workers and the role of learning are often discounted in the current literature. This dissertation explores how the dynamics of cooperation and the learning of preferences as cultural traits become consequential in the evolution and longevity …


Risk-Prone And Risk-Averse Foraging Strategies Enable Niche Partitioning By Two Diurnal Orb-Weaving Spider Species, Mitchell D. Long May 2022

Risk-Prone And Risk-Averse Foraging Strategies Enable Niche Partitioning By Two Diurnal Orb-Weaving Spider Species, Mitchell D. Long

Electronic Theses and Dissertations

Niche partitioning is a major component in understanding community ecology and how ecologically similar species coexist. Temporal and spatial partitioning and differences in foraging strategy, including sensitivity to risk (variance), likely contribute to partitioning as well. Here, we approach this partitioning with fine resolution to investigate differences in overall strategy between two species of diurnal, orb-weaving spiders, Verrucosa arenata and Micrathena gracilis (Araneae: Araneidae), that share similar spatial positioning, temporal foraging window, and prey. Through field observation, we found that V. arenata individuals appear to increase spatial and temporal sampling to compensate for an overall risk-prone strategy that depends on …


Morphological Variation And Community Science In Orthoptera, Amy Byerly Jan 2022

Morphological Variation And Community Science In Orthoptera, Amy Byerly

Electronic Theses and Dissertations

Patterns of morphological divergence across species’ ranges provide insight into local adaptation and speciation. Here, we compare phenotypic divergence among 4,221 crickets from 337 populations of two related species of field cricket, Gryllus firmus and G. pennsylvanicus and their hybrids. We find that these species differ across their geographic range in key morphological traits, such as body size and ovipositor length, and we directly compare phenotype with genotype for a subset of crickets demonstrating nuclear genetic introgression, phenotypic intermediacy of hybrids, and essentially unidirectional mitochondrial introgression. We discuss how these morphological traits relate to life history differences between the species. …


Population Genomics Of Redbreast Sunfish (Lepomis Auritus): Exploring Gene Flow And Local Adaptation In A Widely Distributed Freshwater Fish, Garret J. Strickland Jan 2022

Population Genomics Of Redbreast Sunfish (Lepomis Auritus): Exploring Gene Flow And Local Adaptation In A Widely Distributed Freshwater Fish, Garret J. Strickland

Electronic Theses and Dissertations

Little information is available concerning the distribution of genetic diversity in non-salmonid, non-imperiled, freshwater fish. In order to fill in this knowledge gap, I conducted a population genomics survey in Redbreast Sunfish (Lepomis auritus; RBS), a widespread, generalist species distributed along the Atlantic slope rivers of eastern North America. I sampled four basins (ACF, Savannah, Roanoke, and James) at eight sites each with a factorial experimental design. Sites were distributed among coastal plain, Piedmont, or mountain ecoregions in order to capture the greatest range of environmental states experienced by RBS, with the intention of finding evidence for local adaptation to …


Mortality In Interspecific Hybrids Of Nasonia Vitripennis And Nasonia Giraulti, Bonnie Cobb Jan 2022

Mortality In Interspecific Hybrids Of Nasonia Vitripennis And Nasonia Giraulti, Bonnie Cobb

Electronic Theses and Dissertations

Nasonia is a parasitoid wasp genus that serves as an emerging model for studying speciation due to an incompatibility between nuclear and mitochondrial genomes between sister taxa. Short generation times, easy rearing in a lab setting, producing large amounts of progeny, and whole genome sequencing make Nasonia is an excellent candidate for studying incomplete reproductive isolation. Nasonia have five chromosomes and exhibit haplo-diploid sex determination in which fertilized eggs develop into diploid females and unfertilized eggs develop into haploid males. Recessive phenotypes are hidden in diploid females but are laid bare in haploid males as there is no interaction between …


Lipidomic Analysis Of Various Developmental Stages Of Physcomitrium Patens, Deepshila Gautam Dec 2021

Lipidomic Analysis Of Various Developmental Stages Of Physcomitrium Patens, Deepshila Gautam

Electronic Theses and Dissertations

Lipids maintain fluidity of the cell membrane during the lifetime of all organisms. The moss Physcomitrium patens, an early land plant, enters reproductive phase under cold (15°C) conditions relative to its gametophytes (22°C). Thus, we hypothesized that their lipid content and composition would be distinct. Using ESI-MS/MS, we showed that the content and acyl composition of 11 lipid classes varied during development. Galactolipids were abundant in gametophytes but insignificant in sporophytes; among phospholipids, phosphatidylcholine was predominant in both phases. Although, sporophytes contained around five-fold less lipids than the gametophyte, their phosphatidic acid content, which accumulates during stress, was 18-fold …


The Function And Evolution Of Wood-Warbler Flight Calls (Parulidae), Zach G. Gayk Oct 2021

The Function And Evolution Of Wood-Warbler Flight Calls (Parulidae), Zach G. Gayk

Electronic Theses and Dissertations

Animals produce vocalizations to communicate with territorial rivals, to communicate with potential mates, to alert others of predators, or to stay in contact with other animals. To date, little research has focused on the vocalizations that animals use to communicate during migration. Many birds produce flight calls, which are short calls given by migratory birds on the wing. The function of flight calls is poorly understood but they are thought to help migrants maintain contact with other individuals during movements between breeding grounds and wintering grounds. Across four data chapters in my dissertation, I investigated the function and evolution of …


Comparative Conservation Genomics Of A Suite Of Imperiled Freshwater Mussels, Scott C. Meyer Jan 2021

Comparative Conservation Genomics Of A Suite Of Imperiled Freshwater Mussels, Scott C. Meyer

Electronic Theses and Dissertations

Maintenance of genetic diversity is a critical component to the management and recovery of imperiled species. By ensuring that a species’ genetic diversity is maintained, issues like inbreeding depression and loss of local adaptation can be prevented. However, the genetic diversity of many species are not well-characterized, and the factors that influence a species’ genetic diversity are often not well understood. In the case of imperiled unionid freshwater mussels, it is important to conduct conservation genetic assessments to aid in their management and preserve genetic diversity. This is the first study to conduct a comparative conservation genomic assessment for eight …


The Physiology And Evolution Of Selenite Respiration In Bacteria, Michael Wells May 2020

The Physiology And Evolution Of Selenite Respiration In Bacteria, Michael Wells

Electronic Theses and Dissertations

The selenium oxyanions selenate (Se(VI)) and selenite (Se(IV)) can be utilized by some bacteria and archaea as terminal electron acceptors in anaerobic respiration. Se(VI) and Se(IV) respiration is mediated by a phylogenetically and ecologically diverse array of organisms, suggesting that selenium respiration is ubiquitous in natural environments. Several respiratory Se(VI) reductases have been characterized in bacteria, revealing that Se(VI) respiration has evolved independently several times in this domain. Se(IV) respiration, in contrast, has yet to be characterized. I have purified and characterized the first respiratory Se(IV) reductase from Bacillus selenitireducens MLS10. The Se(IV) reductase appeared to purify as a single …


A Genomic Analysis Of Bobcat Populations In North America With A Comparison To The Canada Lynx: An Assessment Of Local Adaptation To Unique Ecoregions And Phylogeography, Jennifer C. Broderick May 2020

A Genomic Analysis Of Bobcat Populations In North America With A Comparison To The Canada Lynx: An Assessment Of Local Adaptation To Unique Ecoregions And Phylogeography, Jennifer C. Broderick

Electronic Theses and Dissertations

Bobcats (Lynx rufus) are an ecologically and genetically diverse species with a large contiguous range throughout North America. The species not only has a wide array of phenotypic variation compared to other mammals, but shows marked adaptability across ecozones with differing ecological influences. It is these various selective pressures in distinctive parts of the continent that have likely led to localized adaptations within the bobcat metapopulations. The species is also marked by its ability to maintain connectivity and populations in anthropogenically developed areas, an advantage it has over other felids, including its close relative the Canada lynx ( …


Encephalization In Commensal Raccoons: A Unique Test Of The Cognitive Buffer Hypothesis, Peter M. Anderson May 2020

Encephalization In Commensal Raccoons: A Unique Test Of The Cognitive Buffer Hypothesis, Peter M. Anderson

Electronic Theses and Dissertations

This study investigated selective pressures associated with encephalization in mammals and discussed broader implications. Relative brain size as measured by EQ (Encephalization Quotient) was compared between ecological categories. Omnivores had higher average EQ than ecological specialists. Since specialists are disproportionately affected by extinction events, selection for ecological generalism is proposed as encephalization mechanism. This mechanism may reinforce the more widely known Cognitive Buffer Hypothesis (CBH)—the idea that possessing relatively large brains has buffered lineages against environmental change. CBH is tested here by comparing EQs in Procyon lotor (raccoon) in urban and rural environments. CBH predicts that raccoons in the most …


Predator-Based Selection And The Impact Of Edge Sympatry On Components Of Coralsnake Mimicry, Lauren E. Wilson Jan 2020

Predator-Based Selection And The Impact Of Edge Sympatry On Components Of Coralsnake Mimicry, Lauren E. Wilson

Electronic Theses and Dissertations

Studying warning coloration and mimicry is an effective way to understand predator-driven selection and phenotypic diversity. The presence (sympatry) or absence (allopatry) of a toxic model plays a role in shaping mimetic phenotypes. However, the impact of edge sympatry and allopatry on predation of mimetic phenotypes is not well understood. We studied coralsnake mimicry to test how edge sympatry and allopatry affect predation on mimetic phenotypes. Specifically, we tested 1) if overall attack rates varied with edge sympatry of coralsnakes 2) which color patterns conferred a fitness advantage 3) which specific mimetic signal components are important in driving predatory attacks …


Plasticity And The Impact Of Increasing Temperature On A Tropical Ectotherm, Adam A. Rosso Jan 2020

Plasticity And The Impact Of Increasing Temperature On A Tropical Ectotherm, Adam A. Rosso

Electronic Theses and Dissertations

Organisms may respond to climate change through behavior, genetic adaptation, and/or phenotypic plasticity. Tropical ectotherms are thought to be especially vulnerable to climate change because most have a narrow range of thermal tolerance while living close to their upper thermal tolerance limits. Additionally, many tropical species live in closed-canopy forests, which provide homogenous thermal landscapes that prevent behavioral compensation for stressfully warm temperatures. Finally, tropical ectotherms are thought to have decreased capacity for phenotypic plasticity because they have evolved in thermally stable environments. We tested gene expression patterns and phenotypic plasticity in the Panamanian slender anole by a) measuring changes …


Assessing Migrations And Habitat Connectivity For Two Anadromous Species Following A Major Restoration Effort In The Penobscot River, Maine, George A. Maynard May 2019

Assessing Migrations And Habitat Connectivity For Two Anadromous Species Following A Major Restoration Effort In The Penobscot River, Maine, George A. Maynard

Electronic Theses and Dissertations

The construction of industrial dams across major rivers in New England began in the early 1800s with textile mills in Massachusetts. Because of its legacy of mill dams and log driving dams, New England has the highest density of dams anywhere in North America, averaging one dam for every 44 km2 of drainage area. By the early 1900s, these dams drastically limited migrations by diadromous fishes, resulting in declines in populations of migratory fishes, including Atlantic salmon Salmo salar and American shad Alosa sapidissima. Since that time, different fishway designs and river management plans have been tried around …


Differentiating Black Bears (Ursus Americanus) And Brown Bears (U. Arctos) Using Linear Tooth Measurements And Identification Of Ursids From Oregon Caves National Monument, Emily Bogner May 2019

Differentiating Black Bears (Ursus Americanus) And Brown Bears (U. Arctos) Using Linear Tooth Measurements And Identification Of Ursids From Oregon Caves National Monument, Emily Bogner

Electronic Theses and Dissertations

North American black bears and brown bears can be difficult to distinguish in the fossil record due to similar dental and skeletal morphologies. Challenges identifying ursid material from Oregon Caves National Monument (ORCA) called for an accurate tool to distinguish the species. This study utilized a large database of lower tooth lengths and ratios in an attempt to differentiate black and brown bears in North America. Further, this project examined how these linear measurements differ geographically. Analysis of variance (ANOVA) found significant differences between black and brown bears from across North America for every variable studied. Stepwise discriminant analyses (DA) …


Ecometric Estimation Of Present And Past Climate Of North America Using Crown Heights Of Rodents And Lagomorphs: With Application To The Middle Miocene Climatic Optimum, Julia Schap May 2019

Ecometric Estimation Of Present And Past Climate Of North America Using Crown Heights Of Rodents And Lagomorphs: With Application To The Middle Miocene Climatic Optimum, Julia Schap

Electronic Theses and Dissertations

Continental scale studies on ungulate crown heights in relation to climate and habitat changes have revealed a correlation between increasing hypsodonty and a shift to more arid environments. Small mammals have been shown to adapt to changing habitats millions of years earlier than larger mammals. In this study I examined fossil localities throughout the last 37 Ma across North America. Diversity of rodents and lagomorphs were analyzed through this time period, with examination of community structure characterized by relative percentages of taxa with different crown heights. Overall, a decrease in precipitation and temperature was found across North America from 37 …


Niche Evolution Along A Gradient Of Ecological Specialization, Meaghan Conway Apr 2019

Niche Evolution Along A Gradient Of Ecological Specialization, Meaghan Conway

Electronic Theses and Dissertations

The concept of the ecological niche is fundamental to understanding constraints on species distributions in space and time, and in explaining the origin and maintenance of biodiversity. A niche can be broadly defined to include all of the biotic and abiotic conditions that a species requires to persist. Niche breadth, or the degree of specialization, may influence how labile a species niche is, which can have broad implications for species ability to adapt to environmental change, and for explaining patterns of diversification. I investigated mechanism that facilitate or constrain niche evolution at multiple scales. First, I developed an index of …


The Role Of Local Adaptation On Biting Performance In Trinidadian Guppies, Hannah E. Cohen Jan 2019

The Role Of Local Adaptation On Biting Performance In Trinidadian Guppies, Hannah E. Cohen

Electronic Theses and Dissertations

Divergent selection pressures in populations that occupy different environments can result in phenotypic differentiation in traits that provide a local fitness advantage. Trinidadian guppy (Poecilia reticulata) populations are separated by physical barriers such as waterfalls which result in repeated shifts in selective pressures from predator avoidance in high predation environments towards resource competition in low predation environments. Such shifts in selection pressures have previously shown that a range of locally adapted morphological and behavioral traits are changed. However, the role of local adaptation on biting behaviors remains unclear. I analyzed morphological differences such as body length, jaw position, …


Ecological Consequences Of Personality In A Guild Of Terrestrial Small Mammals: From Trappability To Seed Dispersal, Allison M. Brehm Dec 2018

Ecological Consequences Of Personality In A Guild Of Terrestrial Small Mammals: From Trappability To Seed Dispersal, Allison M. Brehm

Electronic Theses and Dissertations

Individual animals exhibit consistent behavioral tendencies over time and across contexts that have been termed personalities. Personality encapsulates an individual’s unique way of behaving and responding to life’s challenges, and since individuals vary in both personality type and their ability to exhibit behavioural plasticity, there are important links between an individual’s personality and its response to a changing environment; resulting in the study of animal personalities becoming increasingly popular in recent years. Previous research suggests that personality traits measured through standardized behavioural tests predict trappability (i.e. ‘trap happiness’ versus ‘trap shyness’). This relationship has been explored only within single …


Eco-Evolutionary Implications Of Environmental Change Across Heterogeneous Landscapes, Jared J. Homola Aug 2018

Eco-Evolutionary Implications Of Environmental Change Across Heterogeneous Landscapes, Jared J. Homola

Electronic Theses and Dissertations

Species use a variety of mechanisms to adapt to environmental change. These range from spatially tracking optimal environments, to phenotypically plastic responses and evolutionary adaptation. Due to increases in anthropogenic influence on environments, characteristics of change such as their duration and magnitude are undergoing fundamental shifts away from the natural disturbance regimes that shaped species’ evolution. This dissertation uses empirical data and simulation models to examine the ecological and evolutionary consequences of environmental change across real, heterogeneous landscapes for multiple species, with an emphasis on anthropogenic changes. I used landscape genetics to evaluate the effects of urbanization on two native …


Diel Rhythmicity Found In Behavior But Not Biogenic Amine Levels In The Funnel-Web Spider Agelenopsis Pennsylvanica (Araneae, Agelenidae), Alexander E. Demarco May 2018

Diel Rhythmicity Found In Behavior But Not Biogenic Amine Levels In The Funnel-Web Spider Agelenopsis Pennsylvanica (Araneae, Agelenidae), Alexander E. Demarco

Electronic Theses and Dissertations

Quantifying individual differences in behavior and the extent that behavior is influenced by circadian control is of paramount importance in behavioral ecology. In addition, the proximate mechanisms underlying behavior are also critical in order to obtain a more complete picture of how behavior evolves. Biogenic amines (BAs) are simple nitrogenous compounds derived from amino acids and have been consistently and extensively linked to behavior. For this study, we analyzed temporal patterns of BAs in relation to the antipredator (boldness) and aggressive behavior in female Agelenopsis pennsylvanica, a funnel-web spider. Using HPLC-ED, we compared behavioral responses to temporal patterns of …


The Biogeographic Origins And Trophic Ecology Of Maine’S Island Red-Backed Salamanders (Plethodon Cinereus), Nikko-Ideen Shaidani May 2017

The Biogeographic Origins And Trophic Ecology Of Maine’S Island Red-Backed Salamanders (Plethodon Cinereus), Nikko-Ideen Shaidani

Electronic Theses and Dissertations

Island populations of terrestrial species have an increased potential, compared to mainland populations, to adapt and diverge, as these populations often are isolated with respect to gene flow from other populations and may be subjected to novel pressures. Indeed, extended isolation of individuals can elicit dramatic changes within populations and is recognized as a common driver of speciation. It is for these reasons that island populations are often a priority for conservation. Plethodontid salamanders are among the most terrestrial of Maine’s amphibians and are not tolerant of prolonged exposure to seawater, and yet, they are found on a number of …


Cranial Morphological Distinctiveness Between Ursus Arctos And U. Americanus, Benjamin James Hillesheim May 2017

Cranial Morphological Distinctiveness Between Ursus Arctos And U. Americanus, Benjamin James Hillesheim

Electronic Theses and Dissertations

Despite being separated by millions of years of evolution, black bears (Ursus americanus) and brown bears (Ursus arctos) can be difficult to distinguish based on skeletal and dental material alone. Complicating matters, some Late Pleistocene U. americanus are significantly larger in size than their modern relatives, obscuring the identification of the two bears. In the past, fossil bears have been identified based on differences in dental morphology or size. This study used geometric morphometrics to look at overall differences in cranial shape and used step-wise discriminant analysis to identify specific characters that distinguish cranial morphology between …