Life Sciences Commons^™

Trends In Cyanotoxin Production Potential In China Lake: An Edna Analysis Of Microcystis And Dolichospermum And The Environmental Drivers Of Cyanotoxin Production, Gennifer M. Rubin

Honors Theses

Episodes of cyano-harmful algal blooms (cyano-HABs) are hypothesized to be exacerbated by the effects of climate change. However, the dynamics of the interactions between elements of climate change and toxigenic cyanobacteria proliferation are not well defined. China Lake is the drinking water source for 7 municipalities in the Kennebec County and is one of many lakes in central Maine that has been subjected to high levels of cyano-HABs and microcystin toxin contamination in recent years. Monitoring the toxicity of these blooms in relation to various aspects of climate change may lead to identification of the major drivers of microcystin production …

Cytotoxicity And Reproductive Impairment In Rainbow Trout Cell Lines Exposed To Microcystis Aeruginosa Extracellular Metabolites, Keira Harshaw

Electronic Theses and Dissertations

Current trends in cyanobacterial harmful algal blooms (cHABs) demonstrate increasing risks to human health and the health of aquatic ecosystems around the globe. Expansion of algal blooms, both geographically and temporally, serve to place increasing numbers of freshwater species, including fish, in peril. Microcystis aeruginosa, one of the most common species of bloom-causing cyanobacteria, is capable of producing a vast diversity of biologically active compounds, however Microcystis studies are often dominated by microcystins. How non-microcystin metabolites contribute to Microcystis toxicity, particularly in freshwater fish, has been the subject of a limited, but growing, body of research. To contribute to …

Investigation Into Bacterial Impairment Of Greene And Polk County Water Systems, John C. Kincaid

MSU Graduate Theses

Bacterial impairment of water systems is a major issue facing mankind. Bacteria that are introduced into a system have the potential to cause harmful diseases to wildlife and humans. In Greene and Polk counties, many water systems have become bacterially impaired over the years. Despite this, little is known about the contamination of known harmful bacteria in this region. To address this issue, I investigated the presence of known human pathogens across water systems in these two counties, many of which have displayed high levels of E. coli and fecal indicator microorganisms over many years. I used a high-throughput sequencing …

Improvement Of Biochar Through Ozonization And Biosafety Of Genetically Engineered Cyanobacteria, Oumar Sacko

Chemistry & Biochemistry Theses & Dissertations

Through the innovative technique of biochar post-production surface oxygenation by ozonization, we were able to improve certain properties of biochar. In project one, the incubation of an insoluble phosphate rock material (hydroxyapatite) with the wet ozonized pine 400 biochar and its filtrate resulted in a solubilization of 80 times more phosphate from hydroxyapatite (569.0 mg/L ± 6.4) compared to the pure water-hydroxyapatite control (7.2 mg/L ± 0.3). The ozonized biochar may provide a new possible way to unlock the phosphorus from insoluble phosphate mineral phases. The cation exchange capacity (CEC) is a key property of biochar when used as a …

Metabolic Engineering Of Cyanobacteria For Production Of Chemicals, Po-Cheng Lin

McKelvey School of Engineering Theses & Dissertations

Concerns over the impact of climate change caused by CO2 emission have driven the research and development of renewable energies. Microbial production of chemicals is being viewed as a feasible approach to reduce the use of fossil fuels and minimize the impact of climate change. With recent advances in synthetic biology, microorganisms can be engineered to synthesize petroleum-based chemicals and plant-derived compounds. Cyanobacteria are photosynthetic prokaryotes that use only sunlight, CO2, and trace minerals for growth. Compared to other microbial hosts, cyanobacteria are attractive platforms for sustainable bioproduction, because they can directly convert CO2 into products. However, the major challenge …

Metabolic Engineering Of Cyanobacteria For Production Of Chemicals, Po-Cheng Lin

McKelvey School of Engineering Theses & Dissertations

Concerns over the impact of climate change caused by CO2 emission have driven the research and development of renewable energies. Microbial production of chemicals is being viewed as a feasible approach to reduce the use of fossil fuels and minimize the impact of climate change. With recent advances in synthetic biology, microorganisms can be engineered to synthesize petroleum-based chemicals and plant-derived compounds. Cyanobacteria are photosynthetic prokaryotes that use only sunlight, CO2, and trace minerals for growth. Compared to other microbial hosts, cyanobacteria are attractive platforms for sustainable bioproduction, because they can directly convert CO2 into products. However, the major challenge …

Naturalization Of Salt Marsh Restoration Sites In The Elizabeth River, Virginia, Assessed By Feeding Activity And Trophic Level Of Mummichogs (Fundulus Heteroclitus), Moriah Anne Good

Biological Sciences Theses & Dissertations

Recent efforts to mitigate environmental issues within the Southern Branch of the Elizabeth River, Virginia, designated a “Region of Concern” by the Chesapeake Bay Program, include several salt marsh restorations. By examining gut contents and stable isotopes values (δ13C and δ15N) from mummichogs (Fundulus heteroclitus), the functional equivalency of restored salt marshes compared to natural marshes was measured. In July 2013 I collected mummichogs from three restored and three reference salt marshes in the Southern Branch. Fish were collected for gut content analysis and were analyzed for stable isotopes (δ13C and δ15N).

I removed gut contents from 16 fish per …

Assessing The Impacts Of Climate Change On The Surface Temperature Of Inland Lakes In Michigan, Kaitlin Reinl

Dissertations, Master's Theses and Master's Reports

The aim of this study was to validate and apply a lake model for predicting the susceptibility of small inland lakes in Michigan to changes in thermal regime and increased cyanobacteria growth as a result of future climate conditions. The Freshwater Lake Model was selected, tested for sensitivity to various inputs, and validated through comparison to observed conditions. The sensitivity analysis showed that the lake model was most sensitive to solar radiation, air temperature, and air humidity. Comparison of predicted climate data with observed conditions revealed highly variable climate model error. The lake model validation was conducted using 10 lakes …

Identification And Quantification Of Zooplankton In Ne Ohio Drinking Water Reservoirs, Michael Vigorito

Williams Honors College, Honors Research Projects

Cyanobacteria, or blue-green algae, are present in most freshwater ecosystems and are usually harmless. When these algae swell in numbers, they release harmful toxins that can be detrimental to animal and human health, and can destroy ecosystems. For this reason, many scientists and engineers have studied these harmful algal blooms in an attempt to predict, prevent, or control them to keep people and ecosystems safe. One of the variables in this investigation is the presence and quantity of zooplankton. These animals could play an important role in the prevalence of cyanobacteria, but more information is needed to determine what that …