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Full-Text Articles in Life Sciences

The Development Of A Global Shoreland Information Database May Help Determine The Appropriate Bacteria And Nutrient Technology Needed To Remediate Oil Contaminated Shorelands, Tiffany Ann Veeder May 2022

The Development Of A Global Shoreland Information Database May Help Determine The Appropriate Bacteria And Nutrient Technology Needed To Remediate Oil Contaminated Shorelands, Tiffany Ann Veeder

Honors Projects

The use of oil and its derivative products is well known world-wide. The benefits of oil are many and its products, for example, range from gasoline used in our automobiles to its use in plastics, building products, and medical supplies. Oil spill hazards associated with oil extraction, production, and transportation can have devastating impacts on the environment and local economies. When oil spills happen in the water, for instance, major shorelands are placed at risk of significant harm. As oil contaminates shorelands, the impacts can range from killing sea creatures and seabirds to destroying entire ecosystems. To help mitigate the …


Diversity Of Bacteriophage In Burkholderia Species, Abigail Price Apr 2022

Diversity Of Bacteriophage In Burkholderia Species, Abigail Price

Honors Projects

Bacteriophages are viruses that infect and replicate within bacteria and offer the potential of a therapeutic alternative to chronic infections that do not respond to antibiotic-based therapies. B. vietnamiensis is one of a number of Burkholderia species involved with chronic drug resistant infections in the lungs of individuals with compromised respiratory systems, as found in those with chronic obstructive pulmonary disease and, most especially, are of particular significance in patients with cystic fibrosis. The diversity of the Burkholderia species is explored by using online databases and looking at bacteriophage or phage-encoding viruses found in B. vietnamiensis. The open reading frames …


Assessing Environmental Factors That Influence Cyanobacterial Blooms In Skinn Lake, Kloe Atwood Apr 2022

Assessing Environmental Factors That Influence Cyanobacterial Blooms In Skinn Lake, Kloe Atwood

Honors Projects

Global lakes are experiencing an increase in toxic algal blooms that can be damaging to the environment. These blooms are caused by cyanobacteria, specifically a species called Planktothrix. Skinn Lake, in Ohio, is a location of a cyanobacterial bloom that is mainly populated by Planktothrix rubescens. This lake was further examined to identify the major toxin-producing bacteria and identify environmental microbes surrounding the bloom. When the data was examined it was found that the microbiomes of the lake greatly differed between the winter (bloom) months and the summer (non-bloom) months. This difference can be viewed within the nutrient analysis from …


Developing An Electroporation Method For Transforming Streptomyces Nymphaeiformis, Heather Knott, Stephen Baron Apr 2022

Developing An Electroporation Method For Transforming Streptomyces Nymphaeiformis, Heather Knott, Stephen Baron

Honors Projects

Streptomyces species are notoriously difficult to transform. Streptomyces nymphaeiformis is no different, so a method of electroporation was used to attempt to transform the cells. Multiple growth stages were used in order to alter the degree of development of the cell wall. The procedure did not kill the cells, but the cells were not transformed. Due to the lack of transformation with S. nymphaeiformis, transformation was attempted on two other Streptomyces strains, S. lividans and S. coelicolor. Neither was successfully transformed to thiostrepton (tsr) resistance, nor did they grow on a plate lacking thiostrepton. One possibility for the …