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An Anaerobic Methane Oxidizing Community Of Anme-1b Archaea In Hypersaline Gulf Of Mexico Sediments, Karen Lloyd, Laura Lapham, Andreas Teske
An Anaerobic Methane Oxidizing Community Of Anme-1b Archaea In Hypersaline Gulf Of Mexico Sediments, Karen Lloyd, Laura Lapham, Andreas Teske
Karen Lloyd
Sediments overlying a brine pool methane seep in the Gulf of Mexico (Green Canyon 205) were analyzed using molecular and geochemical approaches to identify geochemical controls on microbial community composition and stratification. 16S rRNA gene and rRNA clone libraries, as well as mcrA gene clone libraries, showed that the archaeal community consists predominantly of ANME-1b methane oxidizers; no archaea of other ANME subgroups were found with general and group-specific PCR primers. The ANME-1b community was found in the sulfate-methane interface, where undersaturated methane concentrations of ca. 100 to 250 _M coexist with sulfate concentrations around 10 mM. Clone libraries of …
Desiccation Responses And Survival Of Sinorhizobium Meliloti Usda 1021 In Relation To Growth Phase, Temperature, Chloride And Sulfate Availability, Jan A.C. Vriezen Dr., Frans J. Debruijn Dr., Klaus R. Nusslein Dr.
Desiccation Responses And Survival Of Sinorhizobium Meliloti Usda 1021 In Relation To Growth Phase, Temperature, Chloride And Sulfate Availability, Jan A.C. Vriezen Dr., Frans J. Debruijn Dr., Klaus R. Nusslein Dr.
Jan A.C. Vriezen Dr.
Aims: To identify physical and physiological conditions that affect the survival of Sinorhizobium meliloti USDA 1021 during desiccation. Methods and Results: An assay was developed to study desiccation response of S. meliloti USDA 1021 over a range of environmental conditions. We deter- mined the survival during desiccation in relation to (i) matrices and media, (ii) growth phase, (iii) temperature, and (iv) chloride and sulfate availability. Conclusions: This study indicates that survival of S. meliloti USDA 1021 during desiccation is enhanced: (i) when cells were dried in the stationary phase, (ii) with increasing drying temperature at an optimum of 37°C, and …
Survival And Growth Of Two Heterotrophic Hydrothermal Vent Archaea, Pyrococcus Strain Gb-D And Thermococcus Fumicolans, Under Low Ph And High Sulfide Concentrations In Combination With High Temperature And Pressure Regimes, Virginia P. Edgcomb, Stephen J. Molyneaux, Simone Böer, Carl O. Wirsen, Mark Saito, Michael S. Atkins, Karen Lloyd, Andreas Teske
Survival And Growth Of Two Heterotrophic Hydrothermal Vent Archaea, Pyrococcus Strain Gb-D And Thermococcus Fumicolans, Under Low Ph And High Sulfide Concentrations In Combination With High Temperature And Pressure Regimes, Virginia P. Edgcomb, Stephen J. Molyneaux, Simone Böer, Carl O. Wirsen, Mark Saito, Michael S. Atkins, Karen Lloyd, Andreas Teske
Karen Lloyd
Growth and survival of hyperthermophilic archaea in their extreme hydrothermal vent and subsurface environments are controlled by chemical and physical key parameters. This study examined the effects of elevated sulfide concentrations, temperature, and acidic pH on growth and survival of two hydrothermal vent archaea (Pyrococcus strain GB-D and Thermococcus fumicolans) under high temperature and pressure regimes. These two strains are members of the Thermococcales, a family of hyperthermophilic, heterotrophic, sulfur-reducing archaea that occur in high densities at vent sites. As actively growing cells, these two strains tolerated regimes of pH, pressure, and temperature that were in most cases not tolerated …
Heterotrophic Archaea Dominate Sedimentary Subsurface Ecosystems Off Peru, Jennifer F. Biddle, Julius S. Lipp, Karen Lloyd, Ketil B. Sørensen, Rika Anderson, Helen F. Fredericks, Marcus Elvert, Timothy J. Kelly, Daniel P. Schrag, Mitchell L. Sogin, Jean E. Brenchley, Andreas Teske, Christopher H. House, Kaw-Uwe Hinrichs
Heterotrophic Archaea Dominate Sedimentary Subsurface Ecosystems Off Peru, Jennifer F. Biddle, Julius S. Lipp, Karen Lloyd, Ketil B. Sørensen, Rika Anderson, Helen F. Fredericks, Marcus Elvert, Timothy J. Kelly, Daniel P. Schrag, Mitchell L. Sogin, Jean E. Brenchley, Andreas Teske, Christopher H. House, Kaw-Uwe Hinrichs
Karen Lloyd
Studies of deeply buried, sedimentary microbial communities and associated biogeochemical processes during Ocean Drilling Program Leg 201 showed elevated prokaryotic cell numbers in sediment layers where methane is consumed anaerobically at the expense of sulfate. Here, we show that extractable archaeal rRNA, selecting only for active community members in these ecosystems, is dominated by sequences of uncultivated Archaea affiliated with the Marine Benthic Group B and the Miscellaneous Crenarchaeotal Group, whereas known methanotrophic Archaea are not detectable. Carbon flow reconstructions based on stable isotopic compositions of whole archaeal cells, intact archaeal membrane lipids, and other sedimentary carbon pools indicate that …